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Nucleic acid structure detection method based on surface enhanced Raman spectroscopy

A surface-enhanced Raman and spectral detection technology, used in Raman scattering, measurement devices, material analysis by optical means, etc. Reproducibility and credibility, the effect of improving accuracy

Active Publication Date: 2018-08-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, it is relatively difficult to apply surface-enhanced Raman technology to the field of quantitative analysis, because the conditions of surface-enhanced Raman are not easy to control, and there are many types of substrates, and different factors such as the surface roughness of metal nanoparticles will affect the intensity of surface-enhanced Raman spectra. influences
The existing technology relies on the strong interaction between the sequence containing the marker and the substrate to quantitatively analyze the nucleic acid secondary structure, but the marker itself may have an impact on the formation of the nucleic acid secondary structure
Therefore, the real structure of the nucleic acid cannot be truly detected, and the cost is relatively high
Although surface-enhanced Raman spectroscopy has been successfully applied to label-free analysis of oligonucleotides and single-stranded DNA, detection of complex DNA secondary structures remains a challenge

Method used

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  • Nucleic acid structure detection method based on surface enhanced Raman spectroscopy
  • Nucleic acid structure detection method based on surface enhanced Raman spectroscopy
  • Nucleic acid structure detection method based on surface enhanced Raman spectroscopy

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Embodiment 1

[0027] DNA generally exists in the form of double strands, but also forms triple-stranded structures and four-stranded structures. Such as figure 1 As shown, G-quadruplex is a kind of DNA four-strand structure, which is formed by the sequence rich in G (guanine) in Na+ or K+ solution, and each layer in the structure is between G and G The G-quartet is formed through Hoogsteen hydrogen bonds, and each base is a hydrogen bond donor and acceptor. This structure can consist of 4, 2 or 1 nucleotide chains. G-quadruplex is usually formed by stacking two or more G-quartets through planar bases. G-quadruplex is a ubiquitous DNA secondary structure in the human body. Detecting its structure includes the following steps:

[0028] (1) The preparation method of silver sol is with reference to classical lee method, and in the silver nitrate that 0.036g is dissolved in 200mL water, add the sodium citrate that 4ml mass concentration is 1%, the ultraviolet maximum absorption value of the ...

Embodiment 2

[0034] The difference between this embodiment and Embodiment 1 is that the pH of the buffer solution added in step (2) is 3.0.

Embodiment 3

[0036] The difference between this embodiment and Embodiment 1 is that the pH of the buffer solution added in step (2) is 5.0.

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Abstract

The invention relates to a nucleic acid structure detection method based on surface enhanced Raman spectroscopy. The nucleic acid structure detection method comprises: (1) preparing a silver nanoparticle sol modified and cleaned with iodine ions; and (2) adding a to-be-detected DNA sample to a centrifuge tube filled with the iodine ion modified silver nanoparticle, adding a buffer solution with the PH value of 3.0-5.0, adding an aluminum ion or titanium ion aggregating agent, and carrying out surface enhanced Raman spectroscopy detection. According to the present invention, the method effectively detect the secondary structure of nucleic acid, has the advantages of simpleness, rapidness and the like of SERS, and further has extremely high reproducibility and extremely high reliability; themethod overcomes the difficulty in the detection of the secondary structure of nucleic acid in the traditional SERS detection, has advantages of simple operation steps, rapidness, high sensitivity and good reproducibility, can obtain the signal with the extremely high signal-to-noise ratio, and achieves the high sensitivity detection on various nucleic acid secondary structures.

Description

technical field [0001] The invention belongs to the field of nucleic acid structure detection, and in particular relates to a method for detecting nucleic acid structure based on surface-enhanced Raman spectroscopy. Background technique [0002] Deoxyribonucleic acid, also known as deoxyribonucleic acid, is a biological macromolecule that can form genetic instructions to guide biological development and life function operations. The main function is information storage, which can be compared to "blueprint" or "recipe". It contains instructions needed to build other compounds in the cell, such as proteins and ribonucleic acid. A segment of DNA that codes for a protein is called a gene. In this paper, the SERS method is used to reveal many details of the secondary structure of nucleic acid at the molecular level, which provides a new method for better studying the structure of nucleic acid. [0003] Surface-enhanced Raman spectroscopy (SERS), as a fingerprint spectrum, is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 国新华李洋韩晓霞赵冰
Owner JILIN UNIV
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