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Lateral flow immune test strip based on ordered micro-nano structure

A technology of micro-nano structure and test strips, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of affecting the uniform distribution of nanoparticles, reducing the efficiency of immune reaction, and reducing the reaction time, so as to improve the linear detection range, The effect of improving detection sensitivity and accelerating reaction

Pending Publication Date: 2020-10-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing vertical flow detection test strips only consist of a detection pad and a water-absorbent pad. When the sample is dropped on the detection area, it will flow through the detection area quickly under the action of the water-absorbent pad, causing the sample to be in the detection area. The reaction time is reduced, thereby reducing the efficiency of the immune response
In addition, the chromatographic pads in the vertical flow chromatography test strips at this stage are cellulose acetate membranes or nitrocellulose membranes, and their pores are irregular porous structures at the micron level.
On the one hand, the irregular large pore size causes the sample to flow through quickly, reducing the reaction efficiency; on the other hand, it also affects the uniform distribution of nanoparticles on it and affects the detection signal

Method used

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Embodiment 1

[0039] A lateral flow immune test strip based on ordered micro-nano structure for the multi-target detection of hepatitis B surface antigen (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV), including the following steps:

[0040] 1) Lateral flow immunoassay strip based on ordered micro-nano structure: choose organic plastic substrate 1 (length: 110mm, width: 5mm), glass fiber sample pad 2 (length: 30mm, width: 5mm), glass fiber Conjugate pad 3 (length: 7mm, width: 5mm), nitrocellulose chromatography pad 4 (length: 30mm, width: 5mm), absorbent pad 7 is absorbent paper (length: 30mm, width: 5mm), based on porous Anodized aluminum ordered micro-nano structure detection pad 6 (hole diameter: 350nm, hole period: 150nm, hole depth: 60μm, length: 10mm, width: 5mm).

[0041] 2) Chemical modification of the ordered micro-nano structure detection pad 6: take the porous anodized aluminum and boil it in boiling hydrogen peroxide for 30 minutes, so that the hydroxyl gr...

Embodiment 2

[0050] A lateral flow immunoassay strip based on an ordered micro-nano structure is used for the detection of C-reactive protein, interleukin 6, serum amyloid and procalcitonin multivariate inflammatory biomarkers, comprising the following steps:

[0051] 1) Construction of lateral flow immunoassay strips based on ordered micro-nano structure: use organic plastic PVC as the back substrate 1 (length: 70mm, width: 5mm), polyvinyl alcohol as the sample pad 2 (length: 30mm , width: 5mm), polyvinyl acetate as conjugate pad 3 (length: 7mm, width: 5mm), cellulose acetate as chromatography pad 4 (length: 30mm, width: 5mm), absorbent pad 7 as absorbent sponge ( Length: 30mm, width: 5mm), inverse opal photonic crystal prepared from polymethyl methacrylate as an ordered micro-nano structure detection pad 6 (hole diameter: 300nm, hole period: 100nm, hole depth: 40μm, Length: 10mm, width: 5mm), in which the length of the upper and lower overlapping parts of the sample pad 2 and the conjuga...

Embodiment 3

[0057] A lateral flow immunoassay strip based on an ordered micro-nano structure is used for the detection of prostate-specific antigen (PSA) biomarkers, comprising the following steps:

[0058] 1) Construction of the lateral flow immunoassay strip based on the ordered micro-nano structure: choose soft organic plastic as the back substrate 1 (length: 50mm, width: 3mm), cellulose film as the sample pad 2 (length: 10mm , width: 3mm), non-woven fabric is conjugate pad 3 (length: 5mm, width: 3mm), polyvinylidene fluoride is chromatography pad 4 (length: 20mm, width: 3mm), absorbent pad 7 is cellulose -Glass fiber composite (length: 15mm, width: 3mm), inverse opal structure photonic crystal film prepared from cellulose acetate film is an ordered micro-nano structure detection pad 6 (hole diameter: 400nm, hole period: 150nm, Hole depth: 50μm, length: 10mm, width: 3mm), where the edges of sample pad 2 and conjugate pad 3, conjugate pad 3 and chromatography pad 4, chromatography pad 4...

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Abstract

The invention discloses a lateral flow immune test strip based on an ordered micro-nano structure. The lateral flow immune test strip is used for detecting a target in an analyte. According to the test strip, a base is used as a substrate, a sample pad, a conjugate pad, a chromatography pad, an ordered micro-nano structure detection pad and an absorption pad are sequentially distributed on the substrate from left to right, the sample pad is a target loading area, nanoparticles capable of being coupled with a target are combined on the conjugate pad, the ordered micro-nano structure detection pad is arranged on the chromatography pad, and a detection area and a quality control area are distributed on the ordered micro-nano structure detection pad; the sample pad and the conjugate pad are overlapped together, the conjugate pad and the chromatography pad are overlapped together, the ordered micro-nano structure detection pad is fixed on the chromatography pad, and the chromatography pad and the absorption pad are overlapped together; in practical application, when a sample reaches a detection area, multi-target detection can be realized by collecting signals; the test strip has the advantages of short detection time, simplicity and convenience in operation, low cost, simultaneous detection of multiple targets and the like, and is expected to be widely applied to the field of rapiddetection.

Description

technical field [0001] The invention relates to a lateral flow immunological test strip based on an ordered micro-nano structure, which belongs to the field of instant detection. Background technique [0002] In the early 1970s, two novel rapid immunodiagnostic methods emerged, namely the lateral flow assay and the vertical flow assay based on immunochromatography. These two methods account for a large share of the point-of-care testing (POCT) medical market due to their ease of operation and fast analysis speed, as well as their application in home healthcare testing. The design of the lateral flow immunoassay platform is based on the integration of different membrane modules in a series of lateral ways, and there are small overlapping connections between each membrane, and each membrane module can perform specific analysis tasks, which makes The operation of the lateral flow assay is simple. All analytical tests can quickly obtain analytical results with only one step of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558G01N33/54346G01N33/54326G01N33/543
Inventor 赵祥伟陈睿鹏
Owner SOUTHEAST UNIV
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