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A Raman sensing analysis method for detecting enterotoxin

An analytical method and enterotoxin technology, applied in the field of optical analysis, to achieve the effect of improving sensitivity, reducing influence and sensitive detection

Active Publication Date: 2022-02-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has strong sensitivity and strong specificity

Method used

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  • A Raman sensing analysis method for detecting enterotoxin
  • A Raman sensing analysis method for detecting enterotoxin
  • A Raman sensing analysis method for detecting enterotoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation of plasmonic nanomaterial Au-Ag Janus NPs:

[0032] ① Preparation of monodisperse Au NPs:

[0033] Au NPs were prepared as seeds by citric acid reduction method. 0.8mL HAuCl 4 Mix with ultrapure water and heat to boiling. Quickly add 0.9mL sodium citrate solution under vigorous stirring, and the mixed solution continues to boil for 15min. Then cool down to room temperature naturally, and centrifuge and wash with ultrapure water to obtain Au NPs;

[0034] ② Preparation of Au-Ag Janus NPs:

[0035] Under vigorous stirring, 20 μL of 0.8 mM 2-mercaptobenzimidazole-5-carboxylic acid (MBIA) was added to 1 mL of citric acid-stabilized Au NPs synthesized in step (1). The mixed solution was incubated in a water bath at 60°C for 2h. After the reaction, the solution was cooled to room temperature, and 50 μL of 8 mM hydroquinone (HQ) and AgNO at a final concentration of 50 μM were added at a constant speed under vigorous stirring. 3 solution. The resulting mi...

Embodiment 2

[0046] 1. Preparation of plasmonic nanomaterial Au-Ag Janus NPs:

[0047] ① Preparation of monodisperse Au NPs:

[0048] Au NPs were prepared as seeds by citric acid reduction method. 1.0mL HAuCl 4 Mix with ultrapure water and heat to boiling. Quickly add 1.0mL sodium citrate solution under vigorous stirring, and the mixed solution continues to boil for 17.5min. Then cool down to room temperature naturally, and centrifuge and wash with ultrapure water to obtain Au NPs;

[0049] ② Preparation of Au-Ag Janus NPs:

[0050] Under vigorous stirring, 22 μL of 1 mM 2-mercaptobenzimidazole-5-carboxylic acid (MBIA) was added to 1.1 mL of citric acid-stabilized Au NPs synthesized in step (1). The mixed solution was incubated in a water bath at 65°C for 2.5h. After the reaction, the solution was cooled to room temperature, and 60 μL of 10 mM hydroquinone (HQ) and AgNO with a final concentration of 52.5 μM were added at a constant speed under vigorous stirring. 3 solution. The res...

Embodiment 3

[0061] 1. Preparation of plasmonic nanomaterial Au-Ag Janus NPs:

[0062] ① Preparation of monodisperse Au NPs:

[0063] Au NPs were prepared as seeds by citric acid reduction method. 1.2mL HAuCl 4 Mix with ultrapure water and heat to boiling. Quickly add 1.1mL sodium citrate solution under vigorous stirring, and the mixed solution continues to boil for 20min. Then cool down to room temperature naturally, and centrifuge and wash with ultrapure water to obtain Au NPs;

[0064] ② Preparation of Au-Ag Janus NPs:

[0065] Under vigorous stirring, 24 μL of 1.2 mM 2-mercaptobenzimidazole-5-carboxylic acid (MBIA) was added to 1.2 mL of citric acid-stabilized Au NPs synthesized in step (1). The mixed solution was incubated in a water bath at 70°C for 3h. After the reaction, the solution was cooled to room temperature, and 70 μL of 12 mM hydroquinone (HQ) and AgNO with a final concentration of 55 μM were added at a constant speed under vigorous stirring. 3 solution. The resulti...

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Abstract

The invention provides a Raman sensing analysis method for detecting enterotoxin, which belongs to the technical field of optical analysis. Comprise the following steps: cesium bromide (CsBr) and lead bromide (PbBr 2 ) powder doped in mesoporous silica (MSNs) to prepare sterically hindered CsPbBr via high-temperature desolvation 3 @MSNs. Ag islands were grown on the Au core via the ligand 2‑mercaptobenzimidazole‑5‑carboxylic acid (MBIA) to synthesize Au‑Ag Janus NPs. Furthermore, the ligand MBIA acts as a Raman beacon molecule, exhibiting a very unique and stable Raman signal. Au‑Ag Janus NPs and CsPbBr 3 @MSNs can greatly amplify the SERS signal through the composite material formed by antigen-antibody. The Raman signal of MBIA is used as the detection signal to avoid additional modification of the Raman beacon. The detection method of the invention has high sensitivity and strong specificity.

Description

technical field [0001] The invention belongs to the technical field of optical analysis, in particular to a Raman sensing analysis method for detecting enterotoxin. Background technique [0002] Staphylococcus aureus enterotoxins (SEs, including SEA, SEB, SEC, SED, and SEE) are considered to be common toxins that cause food poisoning, causing bacterial food poisoning, bacteremia, skin tissue infection, and other diseases. It is not allowed to be detected in food. As the most common Staphylococcus aureus enterotoxins in dairy products and the environment, sensitive and accurate detection of SEs is of great significance for food safety. At present, the methods for detecting enterotoxin mainly include: enzyme-linked immunoassay, mass spectrometry, PCR, electrochemical immunoassay, etc., but there are disadvantages such as high detection limit, cumbersome operation, expensive reagent equipment, time-consuming, and low sensitivity. Food safety poses an urgent requirement for ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N33/543G01N33/68
CPCG01N21/658G01N33/68G01N33/54346G01N2333/31
Inventor 赵媛施丽霞
Owner JIANGNAN UNIV
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