Preparation and application based on composite magnetic SERS platform

A substrate and reaction technology, applied in the field of analytical chemistry, can solve the problems of application limitation and low scattering cross section, and achieve the effect of lowering detection limit, improving sensitivity and excellent enrichment ability.

Inactive Publication Date: 2021-02-05
UNIV OF JINAN
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Problems solved by technology

[0002] Raman spectroscopy has incomparable advantages in multiplex molecular identification due to its inherently narrow spectrum, but its application is limited by its low scattering cross section

Method used

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Examples

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Embodiment Construction

[0020] (1) Fe 3 o 4 Preparation of @GO@Ag substrate: Add 0.65 g FeCl3·6H2O and 0.2 g trisodium citrate into 20 mL of ethylene glycol, after completely dissolving, add 1.2 g of anhydrous sodium acetate under stirring, continue stirring for 0.5 h The mixed solution was transferred to a 50 mL polytetrafluoroethylene autoclave, and reacted in a thermostat at 200 °C for 10 h; after the reaction, it was naturally cooled to room temperature and separated by a magnet to obtain black Fe 3 o 4 , discard the supernatant, wash the product with ethanol for 3 to 5 times, then wash with water for 3 to 5 times, and dry in a vacuum oven;

[0021] Take 1.5 g of the above Fe 3 o 4 Add 50 mL of ethanol, sonicate for 5 min, then add 100 mL of aminopropyltrimethylsilane (APTMS) to the above solution, stir at room temperature for 2 h, then heat, condense and reflux for 2 h, after natural cooling, wash with ethanol Several times to remove excess APTMS, separate with a magnet, and dry in a vacuum...

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Abstract

The invention discloses preparation and application of a Fe3O4@GO@Ag and gold nanostar SERS substrate. The preparation comprises the steps of synthesizing magnetic ferroferric oxide nanoparticles by an improved solvothermal method, carrying out amination modification on the magnetic ferroferric oxide nanoparticles, and coating a graphene oxide shell on the outer layer by electrostatic adsorption,wherein silver nanoparticles can be nucleated and grown in situ by a large specific surface area and a large number of oxygen-containing functional groups on the surface, so that a core-shell satellite structure is realized; the graphene oxide has obvious Raman peaks (G peak, D peak and 2D peak) and can be used as a stable internal standard to reduce experimental errors; the gold nanostars are synthesized through simple chemical reduction and are connected to Raman beacon molecules with Raman signals in a biological silence area through gold-sulfur bonds; and after the two substrates are modified with a primary antibody and a secondary antibody respectively, rapid and sensitive detection of the antigen can be realized. The method is simple in synthesis, easy to separate and high in sensitivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to the preparation and potential application of a magnetic SERS substrate and a gold nano-star substrate. Background technique [0002] Raman spectroscopy has incomparable advantages in multiple molecular identification due to its inherent narrow spectrum, but its application is limited by its low scattering cross section. The discovery of the phenomenon of surface-enhanced Raman scattering (SERS) overcomes this limitation, opening the door for applications in the detection of trace analytes. The main contribution of this enhancement effect comes from the enhanced local electromagnetic field, due to the occurrence of plasmon resonance on the excited surface of the excited noble metal nanocrystal, and then the radiation dipole field causes the electromagnetic field redistribution near the noble metal, which greatly enhances the electromagnetic field in the nanocrystal. The electr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65C01G49/08C01B32/198B82Y30/00
CPCB82Y30/00C01G49/08C01B32/198G01N21/65G01N21/658G01N2021/655
Inventor 颜梅郝梦娇张晶赵悦英魏全勇于京华苗培
Owner UNIV OF JINAN
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