Preparation method for graphene-based surface enhanced Raman scattering probe

A surface-enhanced Raman and graphene surface technology, applied in the fields of nanomaterials and bioanalytical chemistry, can solve the problems such as sensitivity, stability and biocompatibility need to be further improved, and the preparation method is cumbersome, and is environmentally friendly and repeatable. The effect of good sex and simple operation

Inactive Publication Date: 2014-06-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there have been a lot of reports on the structure and preparation methods of SERS probes, there are not many probes suitable for living organisms, and the preparation methods are relatively cumbersome, and the sensitivity, stability and biocompatibility need to be further improved.

Method used

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  • Preparation method for graphene-based surface enhanced Raman scattering probe
  • Preparation method for graphene-based surface enhanced Raman scattering probe
  • Preparation method for graphene-based surface enhanced Raman scattering probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 uses graphene-gold nanoparticles, graphene-silver nanoparticles, and example 2 uses the composite structure of graphene-gold nanorods as the SERS substrate, and 5,5 dithiobis(2-nitrobenzoic acid ) (DTNB) will be described as an example of a SERS marker.

[0025] Example 1

[0026] Prepare the graphene-metal nanoparticle composite structure as the SERS substrate, 5,5 dithiobis(2-nitrobenzoic acid) (DTNB) as the SERS probe, the metal nanoparticles are gold nanoparticles, gold Core silver-shell nanoparticles, the method comprises the steps of:

[0027] 1) Preparation of gold nanoparticles: Add 0.5ml of 1% tetrachloroauric acid to 50 ml of deionized water, heat to boiling under constant stirring, then add 0.5ml of 1% sodium citrate, continue stirring and heating, and keep After boiling for 15 minutes, stop heating, continue to stir, cool to room temperature, and set aside. The diameter of the obtained gold nanoparticles is about 48 nm.

[0028] Preparatio...

Embodiment 2

[0038] The composite structure of graphene-gold nanorods is used as the SERS substrate, and 5,5 dithiobis(2-nitrobenzoic acid) (DTNB) is used as the probe of the SERS marker. The method includes the following steps:

[0039] 1) Prepare polystyrene sulfonate sodium (PSS) modified gold nanorod solution and label Raman molecules. To prepare gold seeds first, mix 2.5 ml of 0.2 M cetyltrimethylammonium bromide (CTAB) solution with 1.5 ml of 1.0 mM tetrachloroauric acid solution at room temperature, stir vigorously and add 0.6 ml of ice-cold 0.01 M hydroboration Sodium solution, stop stirring after 2 minutes to obtain a brown-yellow seed solution. Then prepare the growth solution, add the following reagents in sequence in 50ml 0.2M CTAB solution at room temperature: 2~4ml 4mM silver nitrate solution, 5ml 15mM tetrachloroauric acid solution, 45ml deionized water, and stir slowly evenly. Then add 1.5ml~3ml 0.08M ascorbic acid until the solution becomes colorless. Finally, 1ml of see...

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Abstract

The invention relates to a preparation method for a graphene-based surface enhanced Raman scattering (SERS) probe. Compared with the traditional SERS probe using metal nanoparticle sol as an enhanced substrate, the SERS probe adopts a graphene-metal nanoparticle composite structure as a surface enhanced Raman substrate, wherein the SERS signal is obviously enhanced and the metal nanoparticles on the surface of the graphene are uniform and controllable. The main reason is that as gold nanoparticles exist on the surface of a silicon wafer dispersively, sufficient 'hot points' cannot be generated among the particles, and the gold nanoparticles gather on the surface of graphene oxide, the electromagnetic enhanced effect is achieved and stronger SERS effect is achieved.

Description

technical field [0001] The invention relates to the fields of nanomaterials and bioanalytical chemistry, in particular to a graphene-based surface-enhanced Raman substrate and a preparation method thereof. The substrate has excellent surface-enhanced Raman characteristics. The preparation method is simple in operation, good in repeatability, low in cost and friendly to the environment. Background technique [0002] As an optical detection technology emerging in recent years, surface-enhanced Raman scattering (SERS) technology has gained rapid development in just a few years after its birth due to the combination of the advantages of traditional Raman scattering and plasmon wave enhancement. develop. SERS breaks through the bottleneck caused by the low scattering cross section of traditional Raman scattering, avoids the problems of photobleaching and the toxicity of fluorescent labels in fluorescence spectral imaging, and is currently the research focus of international atte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 王著元陈辉崔一平宗慎飞
Owner SOUTHEAST UNIV
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