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Making method and application of CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor

A technology of environmental estrogen and photoelectrochemistry, which is applied in the field of biosensing and detection of new functional materials and environmental pollutants, can solve problems such as poor application, high detection limit, complicated operation, etc., and improve photoelectric conversion efficiency , low detection limit and cheap raw materials

Active Publication Date: 2015-01-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for environmental estrogen detection at home and abroad mainly include high performance liquid chromatography, immunological methods, electrochemical methods, biological analysis methods, etc., but most of them need expensive instruments, their operations are complicated, and their detection limits are high, so they cannot be obtained well. Applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation method and application of CdS-sensitized TiO2 environmental estrogen photoelectrochemical sensor

[0041] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water, and dried with nitrogen; take 6 μL, 2mg / mL rhodamine B functionalized TiO 2 The solution was added dropwise to the conductive surface of the conductive glass, dried at room temperature, calcined at 400°C for 30 min, and cooled to obtain rhodamine B-TiO 2 Modified glass electrode;

[0042] (2) In Rhodamine B-TiO 2 On the modified glass electrode surface, add 4 μL 0.1 μg / mL environmental estrogen standard solution dropwise, rinse the electrode surface with ultrapure water, and dry in a refrigerator at 4°C;

[0043] (3) Continue to drop 4 μL of 1% BSA solution to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry in a refrigerator at 4°C;

[0044] (4) Continue to drop 4 μL of the mixture of envi...

Embodiment 2

[0046] Example 2 Preparation method and application of CdS-sensitized TiO2 environmental estrogen photoelectrochemical sensor

[0047] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen; take 6 μL, 3 mg / mL rhodamine B functionalized TiO 2 The solution was added dropwise to the conductive surface of the conductive glass, dried at room temperature, calcined at 450°C for 45 min, and cooled to obtain rhodamine B-TiO 2 Modified glass electrode;

[0048] (2) In Rhodamine B-TiO 2 On the modified glass electrode surface, drop 4 μL of 0.5 μg / mL environmental estrogen standard solution, rinse the electrode surface with ultrapure water, and dry in a refrigerator at 4°C;

[0049] (3) Continue to add 4 μL of BSA solution with a mass fraction of 2% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry in a refrigerator at 4°C;

[0050] (4) Continue to d...

Embodiment 3

[0052] Example 3 Preparation method and application of CdS-sensitized TiO2 environmental estrogen photoelectrochemical sensor

[0053] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen; take 6 μL, 4 mg / mL rhodamine B functionalized TiO 2 The solution was added dropwise to the conductive surface of the conductive glass, dried at room temperature, calcined at 500°C for 60 min, and cooled to obtain rhodamine B-TiO 2 Modified glass electrode;

[0054] (2) In Rhodamine B-TiO 2 On the modified glass electrode surface, drop 4 μL of 1 μg / mL environmental estrogen standard solution, rinse the electrode surface with ultrapure water, and dry in a refrigerator at 4℃;

[0055] (3) Continue to drop 4 μL of 3% BSA solution to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry in a refrigerator at 4°C;

[0056] (4) Continue to drop 4 μL of the mixture of...

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PUM

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Abstract

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

Description

technical field [0001] The invention relates to a CdS sensitized TiO 2 Preparation method and application of photoelectrochemical sensor for environmental estrogen, specifically related to an in-situ generation of CdS sensitized TiO 2 The invention discloses a preparation method and application of a competitive environmental estrogen photoelectrochemical biosensor, belonging to the technical field of new functional materials and environmental pollutant biosensing detection. Background technique [0002] With the rapid development of modern industry and agriculture, a large number of environmentally harmful substances have entered the human living environment along with human production activities, causing great harm to human health. Environmental estrogen refers to chemical substances that exist in the environment and have estrogen-like activity in organisms. After entering the human body, they can produce environmental toxins that mimic estrogen. Trace estrogen in the env...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 杜斌黎荣霞闫涛魏琴朱宝存吴丹
Owner UNIV OF JINAN
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