Preparation method of label-free photoelectrochemical sensor for zearalenone

A zearalenone and photoelectrochemical technology, applied in the direction of material electrochemical variables, can solve the problems of low photoelectric conversion efficiency and large band gap, and achieve the effect of improving sensitivity and enhancing transmission speed

Inactive Publication Date: 2018-03-02
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TiO 2 The band gap is large and can only be excited by ultraviolet lig

Method used

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  • Preparation method of label-free photoelectrochemical sensor for zearalenone
  • Preparation method of label-free photoelectrochemical sensor for zearalenone
  • Preparation method of label-free photoelectrochemical sensor for zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1

[0028] One based on TiO 2 Preparation method of label-free photoelectrochemical sensor of mesoscopic crystal (such as figure 1 Shown):

[0029] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished on the chamois covered with alumina powder, washed with water for a second time to remove the residual powder on the surface, and then moved into an ultrasonic bath for cleaning until it is cleaned. Wash thoroughly with ethanol, dilute acid and water;

[0030] (2) Drop 4μL of RTM suspension with a concentration of 3 mg / mL on the surface of a clean glassy carbon electrode, dry under infrared light, and cool to room temperature;

[0031] (3) Then drip 4μL of 1 mg / mL Cu-Co-Fe solution onto the surface of the modified electrode, dry it under an infrared lamp, and then cool it at room temperature;

[0032] (4) Then drop 4μL of 3mg / mL polylysine solution onto the surface of the modified electrode, dry and cool to obtain a PL...

Example Embodiment

[0034] Example 2

[0035] One based on TiO 2 Mesoscopic crystal photoelectrochemical sensor is used in the detection method of zearalenone, the steps are as follows:

[0036] (1) A three-electrode system was used for the measurement. The PL / Cu-Co-Fe / RTM / Ab / ZEN modified electrode prepared in Example 1 was used as the working electrode, Ag / AgCl was the reference electrode, and the platinum wire electrode was the counter electrode. , Use the photoelectrochemical workstation to detect, set the voltage to 0.1V, switch the lamp every 10s, and filter the monochromatic light excitation light source emitted by the xenon lamp by the monochromator before use;

[0037] (2) In the pH 6.5 PBS buffer solution, the photoelectrochemical workstation is used to detect 1×10 -6 ng / mL-10ng / mL series of standard solutions of zearalenone with different concentrations, by recording the different current signals generated before and after the lamp is turned on and off, draw the working curve. Figure 2 A is ...

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Abstract

The invention discloses a preparation method of a label-free photoelectrochemical sensor for zearalenone. According to a construction method of a sensing interface, a nano copper-cobalt-iron material,polylysine and nano-rutile type TiO2 mesoscopic crystals are taken as construction elements, and a zearalenone antibody (Ab) is further immobilized; due to the surface plasma effect of the nano copper-cobalt-iron material and the excellent conductivity of the polylysine, the sensing interface can accelerate the photo-induced electron transfer rate of the nano-rutile type TiO2 mesoscopic crystalsand improve photocurrent signals of the nano-rutile type TiO2 mesoscopic crystals; furthermore, the polylysine is rich in amino, thus being conducive to the load of the antibody; when the zearalenoneand the immobilized zearalenone antibody generate an immunoreaction, the photoelectric signals at the sensing interface are significantly weakened due to a steric hindrance effect. A label-free photoelectric sensing method established based on the phenomenon can realize high sensitive detection of the zearalenone having a concentration within a range of 1*10<-6>-10ng/mL.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and in particular relates to a label-free photoelectrochemical sensor for the detection of zearalenone. Background technique [0002] Zearalenone (Zearalenone), also known as F-2 toxin, is a metabolite of Gibberella zeae. It is widely distributed in contaminated grains, agricultural by-products, and dairy products, especially corn and its processed products. Zearalenone has reproductive and developmental toxicity, immunotoxicity and strong teratogenic toxicity, etc. It can also affect endocrine and may induce tumors. At present, the bioassay methods for the detection of zearalenone mainly include enzyme-linked immunosorbent assay, electrochemistry, and fluorescence spectroscopy. However, the further enhancement of the sensitivity of these methods and the exploration of a sensing platform with low background signal, good reproducibility, and wide respo...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 戴宏陈四红高利红林燕语
Owner FUJIAN NORMAL UNIV
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