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Making method and application of mycotoxin photoelectrochemical sensor for in situ generation of CdS

A mycotoxin and photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of complex sample pretreatment, large amount of sample required, single target, etc., to achieve shortened production time and high sensitivity and specific detection, the effect of a wide linear range

Active Publication Date: 2015-01-21
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these detection methods have disadvantages such as single target, complex sample pretreatment, cumbersome operation, large amount of sample required, and long time consumption, which cannot well meet the needs of quantitative analysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1Preparation method and application of a photoelectrochemical sensor for in-situ generation of CdS mycotoxins

[0040] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen gas; 6 μL, 2 mg / mL ceria-doped reduced graphene oxide composite nanomaterial was added dropwise to the conductive glass. surface, dried at room temperature, calcined at 400°C for 30min, and cooled to obtain ceria-doped reduced graphene oxide composite nanomaterial GS-CeO 2 Modified glass electrodes;

[0041] (2) In GS-CeO 2 On the modified glass electrode surface, add 5 μL, 0.1 μg / mL mycotoxin antibody solution dropwise, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0042] (3) Continue to drop 3 μL of BSA solution with a mass fraction of 1% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a r...

Embodiment 2

[0046] Example 2 Preparation method and application of a photoelectrochemical sensor for in-situ generation of CdS mycotoxins

[0047] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen gas; 6 μL, 3 mg / mL ceria-doped reduced graphene oxide composite nanomaterial was added dropwise to the conductive surface of the conductive glass. surface, dried at room temperature, calcined at 450 °C for 45 min, and cooled to obtain ceria-doped reduced graphene oxide composite nanomaterial GS-CeO 2 Modified glass electrodes;

[0048] (2) In GS-CeO 2 On the modified glass electrode surface, add 5 μL, 0.5 μg / mL mycotoxin antibody solution dropwise, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0049] (3) Continue to drop 3 μL of BSA solution with a mass fraction of 2% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultra...

Embodiment 3

[0053] Example 3 Preparation method and application of a photoelectrochemical sensor for in-situ generation of CdS mycotoxins

[0054] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen; 6 μL, 4 mg / mL ceria-doped reduced graphene oxide composite nanomaterial was dropped onto the conductive surface of the conductive glass , dried at room temperature, calcined at 500°C for 60 min, and cooled to obtain ceria-doped reduced graphene oxide composite nanomaterial GS-CeO 2 Modified glass electrodes;

[0055] (2) In GS-CeO 2 On the modified glass electrode surface, add 5 μL, 1 μg / mL mycotoxin antibody solution dropwise, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0056] (3) Continue to drop 3 μL of BSA solution with a mass fraction of 3% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry ...

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Abstract

The invention relates to a making method and an application of a mycotoxin photoelectrochemical sensor for in situ generation of CdS. The method uses cerium dioxide doped reduced graphene oxide as an antibody capture substrate, and the cerium dioxide doped reduced graphene oxide has good conductivity and large specific surface area, and can effectively reduce background signals. Na2S is directly dropped to the surface of an electrode with Cd<2+> functionalized porous TiO2 nanoparticles as a semiantigen marker carrier in order to realize in situ generation of narrow band gap CdS with a high photoelectric conversion rate, and a photoelectric current signal can be generated through irridating the CdS by an LED lamp of visible light wavelength. The carrier TiO2 can be well matched with the energy band of the CdS in order to further improve the photoelectric conversion signal of the CdS, so the competitive photoelectrochemical sensor for super sensitive detection of zearalenone, alpha-zeranol, aflatoxin B1, aflatoxin B2, ochratoxin A, ochratoxin B and other mycotoxins is made.

Description

technical field [0001] The invention relates to a preparation method and application of an in-situ generated CdS mycotoxin photoelectrochemical sensor, in particular to a preparation method and application of a competitive mycotoxin photoelectrochemical sensor that generates CdS in situ, and belongs to new functional materials and food safety detection technology field. Background technique [0002] In recent years, food pollution has become increasingly serious and frequent, which not only causes huge economic losses, but also seriously affects human health. Mycotoxin is one of the main food pollutants, it is a secondary metabolite produced by mold or fungus, due to its wide distribution, it is easy to contaminate crops, contaminated food or feed, and animals fed by the feed, etc. Enter the food chain and indirectly enter the human body, eventually causing serious consequences such as neurological and endocrine disorders, immunosuppression, liver and kidney damage, reprodu...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N27/327
CPCG01N27/26G01N33/53G01N33/54346G01N33/5438
Inventor 魏琴黎荣霞杜斌马洪敏吴丹胡丽华
Owner UNIV OF JINAN
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