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A Method for Detecting Collagen Triple Helix Structure Using Disulfide Nanomaterials

A nanomaterial and disulfide technology, which is applied in material testing products, analytical materials, biological tests, etc., can solve the problems of cumbersome time-consuming, low specificity, weak affinity, etc., and achieves low cost, high sensitivity, and strong specificity. Effect

Active Publication Date: 2020-05-15
胶原蛋白(武汉)生物科技有限公司
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Problems solved by technology

However, these methods have defects such as tedious and time-consuming, low specificity, and weak affinity.

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  • A Method for Detecting Collagen Triple Helix Structure Using Disulfide Nanomaterials
  • A Method for Detecting Collagen Triple Helix Structure Using Disulfide Nanomaterials
  • A Method for Detecting Collagen Triple Helix Structure Using Disulfide Nanomaterials

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[0035] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0036] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0037] One of the embodiments of the present invention provides a method for detecting collagen triple helical structure using disulfide nanomaterials, comprising the following steps:

[0038] 1. Preparation of disulfide nanomaterials:

[0039] Using ammonium paratungstate as raw material, the precursor of ammonium thiotungstate is prepared through acid dissolution, ammonia dissolution, hydrogen sulfide saturation, washing, crystallization and other processes; thermal decomposition method is used to prepare tungsten disulfide nanomaterials with nanoscale layered structure, see fi...

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Abstract

A method of detecting a collagen triple helix structure of by utilizing a bisulfide nano-material is disclosed. The method includes adding the bisulfide nano-material into a probe polypeptide solution to form a first system, adding the bisulfide nano-material into a mixture of a probe polypeptide solution and a polypeptide solution to be detected to form a second system, detecting fluorescence intensities of the first and second systems, and comparing the fluorescence intensities to determine whether a probe polypeptide and the polypeptide to be detected in the second system generate a collagen triple helix structure or not, wherein the concentration of the probe polypeptide in the first system is equal to the concentration of the probe polypeptide in the second system, and the probe polypeptide is a collagen polypeptide modified with a fluorescent molecule and having a specific sequence. The collagen triple helix structure can be accurately detected through the bisulfide nano-material in the method. The method has advantages of simple operation, a low cost, convenient detection, high sensitivity, high specificity, and a low sample using amount.

Description

technical field [0001] The invention relates to the technical field of biological detection, more specifically, the invention relates to a method for detecting collagen triple helix structure by using disulfide nanometer material. Background technique [0002] As the most abundant protein in the human body, collagen consists of three lines (Gly-X-Y) n Peptide chains of repeating sequences form a characteristic triple helical structure. Collagen mutation or degradation has been found to be closely related to osteogenesis imperfecta, arthritis, cancer, thrombosis and many other diseases. Collagen (Gly-X-Y) n The highly repetitive sequence makes it difficult to screen antibodies with high specificity for collagen. Through phage display library technology, tumor-targeting peptides that specifically bind to fragments of type IV collagen products cleaved by matrix metalloproteases have been discovered. However, these methods have defects such as tedious and time-consuming, low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N21/64
CPCG01N21/6486G01N33/68
Inventor 肖建喜李婷婷孙秀霞范俊
Owner 胶原蛋白(武汉)生物科技有限公司
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