Method for distinguishing rhizoma atractylodis in traditional Chinese medicine compound

Abstract

The invention discloses a method for distinguishing rhizoma atractylodis in a traditional Chinese medicine compound and particularly relates to a method for thin-layer distinguishing of the rhizoma atractylodis in the traditional Chinese medicine compound. The distinguishing method comprises the following steps that a control medicinal material solution is prepared; a sample solution for test is prepared; a negative sample solution is prepared; sample application is performed on the same silica gel G thin-layer plate by using a capillary tube sample applicator; the thin-layer plate is arranged in a chromatography cylinder, development is performed by using a developing solvent, and the plate is taken out and air-dried; the thin-layer chromatographic plate is inspected, an obtained result is distinguished, main fluorescence spots of the chromatography of the sample solution for test and the control medicinal material are located in the corresponding positions and have same color, and the negative sample is free of main fluorescence spots having the same color. The distinguishing method is simple in process and good in separating effect, has strong specificity, further improves the accuracy of distinguishing the rhizoma atractylodis component in the traditional Chinese medicine compound and has huge popularization and application potential in the drug distinguishing fields relevant to the rhizoma atractylodis.

Description

Technical field [0001] The invention relates to a method for identifying atractylodes in a compound Chinese medicine, in particular to a method for identifying atractylodes in a thin layer of the compound Chinese medicine. Background technique [0002] Atractylodes is the dry rhizome of AtractyLodes Lancea(Thunb.)D C. or AtractyLodes chinensis(DC.)Koidz., which grows in most areas of our country. Atractylodes japonicus is a traditional aromatic medicine for removing dampness, which has the effects of removing dampness and spleen, dispelling wind and cold, and improving eyesight. Indications of dampness, middle coke, abdominal distension, diarrhea, edema, beriberi dysfunction, rheumatism arthralgia, cold and cold, night blindness, faint eyesight. Atractylodes japonicus can also be combined with other medicinal materials to make a variety of traditional Chinese medicine compound varieties, such as Ermiao pills with atractylodes as the king medicine, Huoxiang Zhengqi water / oral liq...

AI Technical Summary

Problems solved by technology

[0003] Modern medical research has found that in the marketed Chinese medicinal compound varieties containing Atractylodes atractylodes (see Table 1), the quality standards of most varieties have not yet involved the identification method of Atractylodes atractylodes, while another part of the varieties involve the identification method of the drug, but their The methods are all for the identification of volatile oil or raw powder of medicinal materials, and there is a report on the identification of ingredients after boiling Atractylodes atractylodes, and the separation effect is not very good.

The applicant once used the identification method in the Atractylodes Pharmacopoeia standard to conduct TLC identification of Canghuoxiaoshisan Atractylodes atractylodes, and the results showed that the subsequent inspection of the method was complicated, and no main spots consistent with the reference medicinal materials were detected in this product

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