Beet syrup adulterated honey identification method

A technology for sugar beet syrup and honey, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of improving efficiency and accuracy, low sample volume and simple operation

Active Publication Date: 2015-09-30
INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to find a kind of accurate and rapid identification method for the adulteration of beet syrup in honey for the sugar beet syrup currently used for honey adulteration

Method used

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  • Beet syrup adulterated honey identification method
  • Beet syrup adulterated honey identification method
  • Beet syrup adulterated honey identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Determination and Identification of Characteristic Markers of Beet Syrup of Example 1

[0026] First step, collection of typical beet syrup and typical pure honey

[0027] Beet syrups from different manufacturers and sources were collected, and a total of 10 kinds of beet syrups commonly used at present were collected. Collect honey from different origins and nectar sources in China. The origins include Liaoning, Heilongjiang, Hebei, Henan, Inner Mongolia, Shandong, Hubei, Anhui and other 26 provinces and cities. There are 12 kinds of flowers, sunflower, milk vetch, sesame, wild osmanthus, etc.

[0028] The second step, the determination of the characteristic peaks of sugar beet syrup

[0029] The collected and processed beet syrup, as well as the collected pure honey, were tested using the LTQ-Orbitrap. Through the comparison of spectra, it was found that all the detected beet syrups contained a large number of ions with a mass-to-charge ratio of 168.1016, while all...

Embodiment 2

[0032] Determination of embodiment 2 sugar beet syrup detection method

[0033] 1. Sample pretreatment

[0034] (1) Dissolution method. Accurately weigh 2.0 (±0.05) g of honey sample into a 10 mL graduated centrifuge tube with stopper, add 20.0 mL of ultrapure water, shake until dissolved, and filter with a 0.2 μm filter membrane; (2) Acetonitrile extraction method. Accurately weigh 2.0 (±0.05) g of honey sample, add 1.0 mL of ultrapure water, vortex to dissolve, then add 5.0 mL of acetonitrile, shake and extract for 10.0 min, centrifuge at 10,000 rpm for 3.0 min, take 2.0 mL of supernatant, and blow dry with nitrogen Afterwards, dilute to 1.0 mL with ultrapure water and filter with a 0.2 μm membrane filter.

[0035] 2. Instrument Analysis Conditions

[0036] Liquid Chromatography-Mass Spectrometry Conditions

[0037] a) Liquid chromatography: Accela (Thermo scientific);

[0038] b) Chromatographic column: Atlantis T3, 3μm, 150×2.1mm (inner diameter) or equivalent;

[00...

Embodiment 3

[0056] Embodiment 3 honey sample and the mensuration of preparation doping sugar beet syrup sample

[0057] Sample source: 40 real honey samples retained from reliable sources over the years, including rapeseed, linden, acacia and other typical honey species, which have been repeatedly verified as pure honey. Five beet syrup samples from syrup manufacturers in different regions. Randomly select one of the beet syrups, and mix them with 15 randomly selected real honey samples according to 5% by weight to make 15 honeys containing 5% beet syrup.

[0058] Detection method: 3-methoxytyramine with a mass-to-charge ratio of 168.1016 was determined by linear ion trap-orbitrap mass spectrometry (LTQ-Orbitrap) in real honey samples, beet syrup samples and honey containing 5% beet syrup It is a characteristic marker of sugar beet syrup, the sample in which the characteristic marker is detected is positive, and the sample in which the characteristic marker is not detected is negative. ...

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Abstract

The invention relates to a beet syrup adulterated honey identification method, and aims to provide an accurate and fast beet syrup adulterated honey identification method to solve the problem that beet syrup is adulterated in the conventional counterfeited honey, and discloses a beet syrup adulterated honey detection method. The beet syrup adulterated honey identification method comprises three steps of pretreatment of a sample, liquid chromatographic separation and mass spectrum detection, and is a liquid chromatography-mass spectrography detection method taking 3-methoxy-tyramine of which the mass-to-charge ratio is 168.1016 as a characteristic marker of beet syrup.

Description

technical field [0001] The invention relates to a method for identifying and detecting whether honey is mixed with sugar beet syrup. Background technique [0002] According to the national standard GB 14963-2011 "National Food Safety Standard Honey", honey is a natural sweet substance that is fully brewed after bees collect nectar / secretion or honeydew from plants, combine with their own secretions. Therefore honey should not be mixed with any foreign substances. However, adulterated honey has been repeatedly banned. Some companies and individuals add sweet substances such as sucrose and syrup to pure honey, and even use sucrose, syrup, flavors, etc. to make "honey", which violates the rights and interests of consumers. Affected the normal development of the honey industry. [0003] The current honey-related standard GB / T 18932.1-2002 can identify the adulteration of C4 plant syrups such as corn syrup in honey. However, since sugar beet belongs to C3 plants, its carbon is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 李响张进杰贾光群曹彦忠刘晓茂魏晓静李学民
Owner INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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