59 results about "P-Tyramine" patented technology

The invention belongs to the technical field of biomedical materials and tissue regeneration, and particularly discloses hydrogel with a self-enhanced piezoelectric effect as well as a preparation method and application of the hydrogel. The hydrogel is prepared by taking microphone (MXene), cellulose nanofiber (CNF), tyramine grafted carboxymethyl cellulose (T-CMC) and regenerated silk fibroin (RSF) as main materials through an enzyme crosslinking reaction of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The hydrogel has excellent mechanical properties, biocompatibility and safety, and can realize self-enhancement in vivo and generate stable piezoelectric signals. The piezoelectric stimulation can promote proliferation, migration and differentiation of osteogenesis-related cells and regulate and control a bone tissue microenvironment, so that formation and repair of new bones in a bone defect area are remarkably accelerated. The hydrogel can be used as an injectable or moldable bone tissue repair material, and is suitable for bone fracture, bone defect and other bone tissue regeneration scenes.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention provides a near-infrared light mediated hybrid hydrogel multi-bioactivity synergistic tissue repair promoting material as well as a preparation method and application thereof, and relates to the technical field of biological materials. The method comprises the following steps: 1) dispersing calcium peroxide in a solvent, and adding a 3-hydroxytyramine hydrochloride solution for reaction to obtain CaO2 (at) PDA nanoparticles; enabling the CaO2 (at) PDA nano-particles to react with a catalase solution, so as to obtain ORN oxygen release particles; (2) mixing the ORN oxygen release particles with the surfaces rich in sulfydryl with Se-NPs with the surfaces rich in selenol after reduction treatment under the protection of inert atmosphere to obtain ORN-coated Se composite particles; the preparation method comprises the following steps: dissolving hyaluronic acid methacrylate in deionized water, adding the ORN-coated Se composite particles, uniformly mixing, adding a photoinitiator, and carrying out ultraviolet curing to obtain the ORN-coated Se-HAMA hydrogel which can realize local response oxygen supply on an anoxic wound surface and has antibacterial and antioxidant characteristics.

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

The application relates to the field of biological preparations, and particularly discloses an immunochromatographic reagent for detecting malignant tumors in urine and application thereof, which is used for solving the problems of poor recognition of tumor specific epitopes, heavy background interference, spectrum overlap and high subjectivity of artificial judgment in the single staining and color development of acridine orange in the detection of urinary exfoliative cells of bladder cancer. The color development reagent comprises A reagent, B reagent, C reagent and D reagent. The A reagent is provided with an antibody complex for target antigens of bladder tumor cells in urine exfoliation and acridine orange. The B reagent comprises a fluorescently labeled secondary antibody composition corresponding to the A reagent and an enzyme-labeled secondary antibody-tyramide deposition amplification composition. In the application, AI imaging analysis is adopted to calibrate layered recognition labels and acridine orange staining negative and positive labels. Through multi-target immunochromatography, two sets of color separation amplification schemes and AI imaging analysis, the application improves the recognition ability of bladder tumor specific epitopes, the image quality and the image analysis accuracy in the detection of urinary exfoliative cells.

The invention relates to a kit and a method for detecting phosphorylated Tau protein. In particular to a p-Tau217 protein detection kit and a p-Tau217 protein detection method. The kit and the method disclosed by the invention are based on the p-Tau217 antibody and the total-Tau protein antibody, and are combined with a TSA technology, so that the specificity, the sensitivity and the accuracy of a detection result are improved, and background signals are effectively reduced.

The application discloses a kind of high adhesion enzymatic hydrogel for promoting vascular intimal repair and a preparation method thereof, and belongs to the technical field of hydrogel preparation.The preparation method is as follows: S1, using methacrylic anhydride to modify the double bond of hyaluronic acid, then crosslinking with dopamine to obtain dopamine-modified double-bonded hyaluronic acid;S2, after removing oxygen from the hyaluronic acid solution, add a crosslinking agent and tyramine hydrochloride, and react in an acidic environment to obtain tyramine-modified hyaluronic acid;S3, mix the dopamine-modified double-bonded hyaluronic acid, tyramine-modified hyaluronic acid and deionized water to obtain a hydrogel precursor solution;S4, then use enzymatic reaction to obtain the hydrogel.The method designed by the application is simple, does not require additional light curing equipment, and the constructed hydrogel is similar to the extracellular matrix.The hydrogel can be coated into different shapes to adapt to different environments.The hydrogel is formed by enzymatic reaction, which is rapid, well adhered to the tissue, biocompatible, and suitable for application in vascular repair.

The present application relates to compounds of formula (Ia) or (Ib): or a salt, solvate, or tautomer thereof; wherein R1, and R2 are independently selected from optionally substituted C1 to C 8 alkyl; or R1 and R2 together with the silicon atom to which they are attached form an optionally substituted 5- or 6-membered ring; R3, and R4 are independently selected from H, optionally substituted C1 to C 8 alkyl; optionally substituted C1 to C 8 haloalkyl; or R3 and R4 together with the nitrogen atom to which they are attached form an optionally substituted azetidine or pyrrolidine ring; R3', and R4' are independently selected from H, optionally substituted C1 to C8 alkyl; optionally substituted C1 to C 8 haloalkyl; or R3' and R4' together with the nitrogen atom to which they are attached form an optionally substituted azetidine or pyrrolidine ring;R5 is selected from a negative charge, H, C1 to C 8 alkyl, or optionally substituted aryl; a is 0 or 1; and Z is a divalent linker group; and to fluorophore compositions comprising such compounds, to assay methods and to kits using such fluorophore compositions.

Disclosed herein are recombinant host cells and methods capable of producing tyramine-containing hydroxycinnamic amide compounds, derivatives, and extracts.SOLUTION: Some embodiments provided include, for example, consumable products containing tyramine-containing hydroxycinnamic acid amides produced by recombinant host cells. Some embodiments provided herein are methods for producing tyramine-containing hydroxycinnamic amide or hydroxycinnamic amide derivatives.SELECTED DRAWING: None

The application discloses a coumarin tyramine glycoside compound and application thereof in preparation of PTP1B inhibitors and anti-diabetic drugs, and a structural formula of the compound is shown in the following formula: wherein R represents a fatty (aromatic) acyl or a monosaccharide substituent, and R' represents a hydrogen atom or an acetyl group; the compound is prepared by taking 4-methoxycoumarin tyramine 4'-acetyl-O-L-rhamnose as a starting material, introducing a fatty (aromatic) acyl or a monosaccharide substituent into a 2-position hydroxyl and a 3-position hydroxyl of L-rhamnose at the same time, and then removing a protecting group of a sugar group. Pharmacological activity tests show that the compound has good inhibitory activity on protein tyrosine phosphatase 1B, and it is indicated that the coumarin tyramine glycoside compound can be researched and utilized as a new type of PTP1B inhibitor and anti-diabetic drug.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

A hydrogel-based biological delivery vehicle used to effectively deliver drug and biological material to tissue or organ sites. More specifically, a hydrogel binding matrix having a biopolymer backbone containing carboxyl groups. Tyramine may be substituted for at least a portion of the carboxyl groups, so that, when hydrogen peroxide is added, it causes creation of covalent bonds between tyramine molecules and cross-links the hydrogel binding matrix, thereby enabling the hydrogel binding matrix to transition from liquid to gel state. The hydrogel binding matrix, in its liquid form, is capable of encapsulating drug reservoirs to create a homogenous liquid with evenly distributed particles containing drugs or target molecules. As the hydrogel binding matrix solidifies into a gel state, the newly created cross-links do not disrupt or react with the drugs or target molecules contained within the drug reservoirs. This hydrogel-based biological delivery vehicle can be used in several medical applications.

The invention relates to the technical field of analysis and detection, in particular to a kit and a method for extracting catecholamine and metabolites thereof based on a paramagnetic particle method and a method for detecting catecholamine and metabolites thereof by LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry). The kit comprises magnetic beads, an activating solution, an eluent, a washing solution, a leacheate I, a leacheate II and a derivatization reagent, the catecholamine and the metabolite thereof comprise one or more of dopamine hydrochloride, epinephrine, noradrenaline hydrochloride, methoxynoradrenaline hydrochloride, methoxyepinephrine hydrochloride and 3-methoxytyramine hydrochloride. The in-vivo concentration of catecholamine and metabolites thereof in human plasma and urine is detected in a high-flux manner by adopting a magnetic bead and derivation method, and the kit has the characteristics of high sensitivity, good repeatability, high accuracy, good specificity and the like.

Disclosed herein are compositions, systems, platforms, and kits comprising tyramine-TCO and a tetrazine molecule linked to a detectable marker. Also disclosed herein are methods of using the compositions, systems, platforms, and kits to detect one or more target molecules, such as proteins or nucleic acids, in a sample.

The present disclosure presents hydrogel compositions for use in the treatment of an ocular disorder (e.g., a retinal disease) and methods of treating an ocular disorder in a subject in need thereof with the hydrogel compositions. The hydrogel compositions can include hydroxyphenylpropionic acid (gelatin-HPA), hyaluronic acid-tyramine (HA-Tyr), a catalyzer (e.g., horseradish peroxidase (HRP)), a cell, a crosslinker (e.g., hydrogen peroxide), or any combination thereof. The methods include administering a therapeutically effective amount of a composition into an eye of the subject, wherein the composition includes any of the hydrogel compositions of the disclosure.

The invention relates to a hole transport layer, a hole transport solution, a perovskite cell, a preparation method of the perovskite cell and a photovoltaic module. The hole transport layer comprises a self-assembly monomolecular material and a co-assembly material, the co-assembly material comprises at least one of tyramine and tyramine salt, and the mass ratio of the co-assembly material to the self-assembly monomolecular material is (0.05-0.3): 1. The phenolic hydroxyl group in the tyramine molecular structure can interact with the terminal group of the self-assembled monomolecular material to guide the self-assembled monomolecular material to be orderly arranged on the substrate, so that defects and pinholes are reduced; a benzene ring structure at the center of a tyramine molecule provides a pi conjugated system which can be subjected to pi-pi stacking interaction with a self-assembled monomolecular material, so that electron coupling among molecules is effectively enhanced, a'bridge 'is erected among the molecules, a two-dimensional or three-dimensional network of hole transport is expanded, the hole mobility of a hole transport layer is effectively improved, and the hole transport efficiency is improved. Therefore, the photoelectric conversion efficiency of the perovskite cell is effectively improved.

The application provides a near-infrared light-mediated hybrid hydrogel multi-biological activity synergistic tissue repair material and a preparation method and application thereof, and relates to the technical field of biological materials. The method comprises the following steps: 1) dispersing calcium peroxide in a solvent, adding 3-hydroxy tyramine hydrochloride solution for reaction to obtain CaO2@PDA nanoparticles; reacting the CaO2@PDA nanoparticles with catalase solution to obtain ORN oxygen-releasing microparticles; 2) mixing the surface thiol-rich ORN oxygen-releasing microparticles and the surface selenol-rich Se-NPs subjected to reduction treatment under the protection of an inert atmosphere to obtain ORN@Se composite microparticles; dissolving hyaluronic acid methacrylate in deionized water, uniformly mixing the ORN@Se composite microparticles, adding a photoinitiator, and performing ultraviolet light curing to obtain an ORN@Se-HAMA hydrogel, which can realize local response oxygen supply for an anoxic wound and has antibacterial and antioxidant properties.

This invention discloses a method for detecting octopamine and tyramine using a laccase-like Schiff base Cu-MOF nanozyme. The method uses a Schiff base formed from glutaraldehyde and 2-aminoterephthalic acid as starting reagents as ligands to synthesize a flower-like Cu-MOF nanozyme. This Cu-MOF exhibits near-infrared light-triggered laccase-like activity. Using octopamine and tyramine as substrates, Cu-MOF catalyzes the oxidation of octopamine and tyramine, conjugating 4-aminoantipyrine to form a red coupling product. This product exhibits a high absorption spectrum at 505 nm, demonstrating high detection efficiency with detection limits of 0.18 μg / mL and 0.60 μg / mL, respectively. X-ray photoelectron spectroscopy analysis shows that the Cu(I) / Cu(II) redox reaction participates in the catalytic process. This method is characterized by high sensitivity, strong specificity, simple operation, and rapid response.

The present disclosure provides a hyaluronic acid-based hydrogel and a preparation method and use thereof. The modified hyaluronic acid hydrogel of the present disclosure is formed by tyramine-modified hyaluronic acid and poly-2-acrylamide-2-methylpropanesulfonic acid sodium-modified hyaluronic acid under the action of horseradish peroxidase and hydrogen peroxide. The crosslinking agent used in the preparation of the modified hyaluronic acid hydrogel of the present disclosure is horseradish peroxidase, and the prepared hydrogel has good biocompatibility; the hydrogel contains a component with a brush structure, and the prepared hydrogel has good lubricity, which is conducive to the treatment of osteoarthritis.

The invention discloses a preparation and synthesis method and application of a tea polysaccharide fluorescence label, and the fluorescence-labeled large yellow tea polysaccharide is generated by reacting carboxyl or reductive aldehyde group in large yellow tea polysaccharide with tyramine to introduce secondary amino group and then carrying out nucleophilic reaction with cyano group in fluorescein isothiocyanate (FITC) under a weak base condition. The marking method has no influence on the overall internal structure and biological activity of the tea polysaccharide, and has the advantages of easily available raw materials, mild reaction conditions, simple and safe operation, low toxicity and the like. Through verification of various technical means such as a small animal living body imaging system, flow cytometry and a laser confocal microscope, the fluorescently-labeled large yellow tea polysaccharide has the remarkable advantages of strong fluorescence signal, high detection sensitivity, good imaging definition and the like, and can be used for research in multiple aspects such as oral absorption, metabolism and excretion, pharmacological efficacy and the like of the tea polysaccharide.

The invention provides a composition comprising oleanolic acid and the salts, prodrug and solvates thereof; and one or more compounds selected from the following group: ellagic acid, adenine, betaine, choline, miquelianin, p-coumaric acid, pipecolic acid, protocatechuic acid and tyramine, and the salts, prodrugs and solvates thereof. Such a composition is for use in reducing lipid levels in sebocytes, and the composition may find use in treating acne vulgaris and rosacea.

This invention discloses an injectable hydrogel loaded with 4-octyl itaconic acid, its preparation method, and its applications, belonging to the field of biomedical materials technology. The injectable hydrogel is formed by enzymatic cross-linking of a hyaluronic acid-tyramine complex to create a three-dimensional network, with 4-octyl itaconic acid encapsulated within this network structure. This invention allows for precise implantation of the hydrogel into the intervertebral disc lesion via minimally invasive injection, achieving local high-concentration drug accumulation. The three-dimensional network of the hydrogel serves as a drug reservoir, enabling continuous and controllable release of 4-OI, prolonging the drug's duration of action and achieving long-term therapeutic effects. Simultaneously, the loaded 4-OI specifically activates the Nrf2 / GPX4 antioxidant pathway in nucleus pulposus cells, mechanistically antagonizing ferroptosis and matrix degradation, thus achieving a dual function of mechanical support and biological therapy. It exhibits good biocompatibility and solves the problems of difficult targeted drug delivery, short-lived efficacy, and lack of disease-modifying effects in existing treatments for intervertebral disc degeneration.

This invention belongs to the field of pharmaceutical technology, specifically relating to the application of N-methyltyramine in the preparation of drugs for improving anger and irritability. The CAS number of the N-methyltyramine (NMT) is 370-98-9. Experimental results show that NMT can directly target and positively regulate the γ-aminobutyric acid type A receptor (GABA). a NMT activates the downstream AC5-cAMP-PKA signaling pathway, thereby improving abnormal emotional states such as anger and depression. Through network pharmacology, molecular docking, micro-thermophoresis experiments, animal behavioral experiments, and cell experiments, NMT significantly improves the behavior of model animals. Therefore, this invention reveals for the first time that NMT improves abnormal emotional states such as anger and depression through GABA. a The mechanism by which R-related signaling pathways play a role in mood regulation can be used to prepare drugs for treating mood disorders such as anxiety, depression, and post-traumatic stress disorder, and has good application prospects.

The present application provides the use of antagonists of tyramine-ADRA2A signaling axis in the preparation of a medicament for preventing and / or treating inflammatory bowel disease. By using antagonists of tyramine-ADRA2A signaling axis, including but not limited to yohimbine, midaglizole and 2-methoxyimidazolidine, the activation of ADRA2A receptors on intestinal stem cells by tyramine can be inhibited, and the proliferation of intestinal stem cells, the repair of damaged intestines and the relief of intestinal inflammation can be restored. The present application lays a new material foundation for the treatment of inflammatory bowel disease, especially Crohn's disease.

The invention discloses a protein marker detection method based on a nano bifunctional compound and loop-mediated isothermal amplification and a product. According to the method, a capture antibody is specifically combined with a to-be-detected protein marker, and a detection antibody modified with HRP or a secondary antibody modified with HRP and the detection antibody are specifically combined with the protein marker, so that an immune complex is formed; the method comprises the following steps: adding H2O2 and a nano bifunctional compound simultaneously loaded with an oligonucleotide initiation sequence and tyramine into a system containing an immune compound, depositing and enriching the nano bifunctional compound on and around the immune compound by utilizing a tyramine deposition reaction catalyzed by HRP, initiating loop-mediated isothermal amplification through the oligonucleotide initiation sequence, and detecting the immune compound by virtue of the loop-mediated isothermal amplification. The real-time quantitative detection on the protein marker is realized. According to the method, tyramine deposition signal amplification and loop-mediated isothermal amplification are effectively linked through the nano bifunctional compound, and the method has the remarkable sensitivity advantage in low-abundance protein marker detection.

The invention discloses an application of a reagent containing multi-copper oxidase in reducing the biogenic amine content of a fermented meat product, recombinant multi-copper oxidase is obtained through a genetic engineering technology, and the multi-copper oxidase is developed into an enzyme reagent for degrading biogenic amine in Sichuan sausages. Results show that the addition of the reagent containing the multi-copper oxidase and the diamine oxidase has no influence on the eating quality and the sensory quality of the Sichuan sausages, in terms of the biogenic amine content, after the reagent is added, the degradation rates of histamine, tyramine, cadaverine and putrescine are at least 49.63%, 38.51%, 38.26% and 35.40%, and the degradation rates of the reagent containing the multi-copper oxidase and the diamine oxidase are at least 49.63%, 38.51%, 38.26% and 35.40%. The reagent provided by the invention has a good degradation effect on four main biogenic amines in Sichuan sausages.

The invention belongs to the technical field of gene engineering, and particularly relates to an acyltransferase gene and application thereof. The invention provides an acyltransferase gene and application thereof. The nucleotide sequence of the acyltransferase gene HOVUSG0796800 is as shown in SEQ ID NO. 1 (sequence identifier number 1). Results of the invention show that the expression quantity of HOVUSG0796800 is significantly increased after a silicon fertilizer is applied, and the gene has significant positive correlation with the content of coumaroyl agmatine, the content of feruloyl tyramine, the content of feruloyl putrescine and the content of coumaroyl tyramine. In-vitro enzyme activity experiments prove that the gene can effectively regulate and control synthesis of the phenol amine substances. Therefore, the HOVUSG0796800 gene has an important function in plant phenol amine metabolism, and has a good application prospect in the aspects of enhancing crop stress resistance and improving plant quality.