110 results about "P-Tyramine" patented technology

The invention discloses a flame-retardant insulating powder coating for a new energy automobile and a preparation method of the flame-retardant insulating powder coating, and belongs to the technical field of coatings. The flame-retardant insulating powder coating for the new energy automobile is prepared from the following raw material components in parts by mass: 380 to 384 parts of epoxy resin, 56 to 58 parts of a toughening agent, 5 to 10 parts of modified flame-retardant filler, 100 to 101 parts of a composite curing agent, 5 parts of a flatting agent, 1 part of benzoin and 0.7 part of an accelerant, the toughening agent is obtained by carrying out a reaction on an isophorone diisocyanate tripolymer, 4-bromophenol and N-p-coumaroyl tyramine; the modified flame-retardant filler is obtained by firstly wrapping hexagonal boron nitride with polydopamine and then modifying with a silane coupling agent; the silane coupling agent is obtained by combining 4-benzonitrile oxygen butyl trimethoxy silane and a sulfydryl silane coupling agent; and the composite curing agent is obtained by combining 4, 4-diaminodiphenylmethane and amino-sulfydryl functional organic polysiloxane.

The invention discloses an NF (nuclear factor)-kappa B inhibitor. The NF-kappa B inhibitor comprises N-trans-p-coumaroyl tyramine; the invention further discloses a preparation method of the NF-kappa B inhibitor. The invention also discloses an application of the NF-kappa B inhibitor in medicines for preventing or treating diseases related to body inflammation. According to the application disclosed by the invention, the influence of the N-trans-p-coumaroyl tyramine on a TLR4 / MyD88 / NF-kappa B inflammation signal channel is detected through an immunofluorescence technology, and a result shows that the N-trans-p-coumaroyl tyramine can be used for reducing the expression of TLR4 and MyD88 proteins and inhibiting the transfer of NF-kappa B proteins to cell nucleuses, so that the N-trans-p-coumaroyl tyramine can be used as an NF-kappa B inhibitor and can be used for preparing an anti-inflammatory drug. The composition is used for preventing or treating body inflammatory response.

The invention discloses a high-adhesion enzymatic hydrogel for promoting vascular intima repair and a preparation method of the high-adhesion enzymatic hydrogel, and belongs to the technical field of hydrogel preparation. The preparation method comprises the following steps: S1, carrying out double-bond modification on hyaluronic acid by utilizing methacrylic anhydride, and then cross-linking with dopamine to prepare dopamine modified double-bond hyaluronic acid; s2, deoxidizing the hyaluronic acid solution, adding a cross-linking agent and tyramine hydrochloride, and reacting in an acidic environment to prepare tyramine modified hyaluronic acid; s3, mixing the dopamine modified double-bond hyaluronic acid, the tyramine modified hyaluronic acid and deionized water to prepare a hydrogel precursor liquid; and S4, carrying out enzymatic reaction to obtain the hydrogel. The method designed by the invention is simple in process, no extra photocuring equipment needs to be added, and the constructed hydrogel is similar to an extracellular matrix. And the coating can be coated into different shapes to adapt to different environments. The gel is formed through enzymatic reaction, and the gel is rapid in gel forming, good in tissue adhesion, good in biocompatibility and suitable for being applied to vascular repair.

The invention belongs to the technical field of biomedical materials and tissue regeneration, and particularly discloses hydrogel with a self-enhanced piezoelectric effect as well as a preparation method and application of the hydrogel. The hydrogel is prepared by taking microphone (MXene), cellulose nanofiber (CNF), tyramine grafted carboxymethyl cellulose (T-CMC) and regenerated silk fibroin (RSF) as main materials through an enzyme crosslinking reaction of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The hydrogel has excellent mechanical properties, biocompatibility and safety, and can realize self-enhancement in vivo and generate stable piezoelectric signals. The piezoelectric stimulation can promote proliferation, migration and differentiation of osteogenesis-related cells and regulate and control a bone tissue microenvironment, so that formation and repair of new bones in a bone defect area are remarkably accelerated. The hydrogel can be used as an injectable or moldable bone tissue repair material, and is suitable for bone fracture, bone defect and other bone tissue regeneration scenes.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses bi-crosslinking self-reinforced hydrogel based on endogenous glucose in-situ initiation as well as a preparation method and application of the bi-crosslinking self-reinforced hydrogel, and relates to the technical field of biomedical materials. The double-crosslinking self-enhanced hydrogel is prepared from the following components in concentration: 1 to 3 weight percent of hyaluronic acid-tyramine, 0.8 to 1.2 weight percent of sodium alginate, 1 to 100 mmol / L of calcium carbonate, 0.0001 to 10 U / mL of horse radish peroxidase, 20 to 40 U / mL of glucose oxidase and the balance of ultrapure water. The invention also discloses an application of the double-crosslinking self-reinforced hydrogel in preparation of a filling and repairing product for soft tissue defects. The hydrogel disclosed by the invention can be filled in a tissue defect part in an injection manner, glucose in body fluid is utilized to initiate crosslinking in situ to form the hydrogel with excellent mechanical properties and long-term stability, and meanwhile, the hydrogel is good in biocompatibility and relatively high in application value.

The invention belongs to the technical field of biology, and particularly discloses application of laccase AmLac in degradation of biogenic amines. The amino acid sequence of the laccase is shown as SEQ ID NO.1. The laccase has different degrees of degradation effects on the biogenic amines such as histamine, spermidine, tyramine, phenylethylamine and putrescine, and the degradation rates of the histamine and the putrescine both reach 100%. The enzyme is stable in enzymatic property, has alcohol tolerance and acid tolerance, has certain adaptive capacity to food matrixes, and can effectively degrade biogenic amines in wine and reduce potential hazards of the biogenic amines to human bodies, so that the safety and stability of products are improved. Therefore, the AmLac has a wide application prospect in the food industry, and especially when the AmLac is used for treating food containing high biogenic amine, the safety quality of the food can be remarkably improved.

The invention discloses a detection method of catecholamine substances and metabolites thereof, freeze-dried powder and application of the freeze-dried powder. The detection method of catecholamine and metabolites thereof comprises the following step: detecting a sample to be detected by adopting a liquid chromatography-tandem mass spectrometry method. According to the present invention, the sample pretreatment and the detection condition optimization are performed so as to achieve the efficient and accurate detection of noradrenaline, epinephrine, variepinephrine, noradrenaline, dopamine and 3-methoxytyramine, the sensitivity is high, the specificity is good, and the new reliable detection method is provided for the detection of catecholamine and metabolites thereof in the blood sample.

The invention provides a near-infrared light mediated hybrid hydrogel multi-bioactivity synergistic tissue repair promoting material as well as a preparation method and application thereof, and relates to the technical field of biological materials. The method comprises the following steps: 1) dispersing calcium peroxide in a solvent, and adding a 3-hydroxytyramine hydrochloride solution for reaction to obtain CaO2 (at) PDA nanoparticles; enabling the CaO2 (at) PDA nano-particles to react with a catalase solution, so as to obtain ORN oxygen release particles; (2) mixing the ORN oxygen release particles with the surfaces rich in sulfydryl with Se-NPs with the surfaces rich in selenol after reduction treatment under the protection of inert atmosphere to obtain ORN-coated Se composite particles; the preparation method comprises the following steps: dissolving hyaluronic acid methacrylate in deionized water, adding the ORN-coated Se composite particles, uniformly mixing, adding a photoinitiator, and carrying out ultraviolet curing to obtain the ORN-coated Se-HAMA hydrogel which can realize local response oxygen supply on an anoxic wound surface and has antibacterial and antioxidant characteristics.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The invention provides a method for synchronously detecting eight catecholamines and metabolites thereof based on liquid chromatography-tandem mass spectrometry and application of the method. The detection method comprises the following steps: mixing a plasma or urine sample to be detected with an internal standard and acetonitrile; centrifuging, taking supernate, blowing liquid with nitrogen, and adjusting the pH value; mixing the supernate with a propionic anhydride solution, and carrying out derivatization reaction to obtain a test solution; performing liquid chromatography-tandem mass spectrometry detection on the test solution and the standard solution, separating dopamine, epinephrine, noradrenaline, epinephrine, noradrenaline, 3-methoxytyramine, homovanillic acid and vanillic mandelic acid by liquid chromatography, and determining the concentration of the standard substance as an X axis by using a mass spectrometry isotope internal standard quantification method. The ratio of the peak area of the standard substance to the peak area of the internal standard substance is the Y axis, establishing a standard curve, and calculating the concentrations of catecholamine and the metabolites thereof in the test solution. The method is high in detection efficiency, high in sensitivity and good in equipment compatibility, the detection time is shortened, and the cost can also be saved.

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

The application relates to the field of biological preparations, and particularly discloses an immunochromatographic reagent for detecting malignant tumors in urine and application thereof, which is used for solving the problems of poor recognition of tumor specific epitopes, heavy background interference, spectrum overlap and high subjectivity of artificial judgment in the single staining and color development of acridine orange in the detection of urinary exfoliative cells of bladder cancer. The color development reagent comprises A reagent, B reagent, C reagent and D reagent. The A reagent is provided with an antibody complex for target antigens of bladder tumor cells in urine exfoliation and acridine orange. The B reagent comprises a fluorescently labeled secondary antibody composition corresponding to the A reagent and an enzyme-labeled secondary antibody-tyramide deposition amplification composition. In the application, AI imaging analysis is adopted to calibrate layered recognition labels and acridine orange staining negative and positive labels. Through multi-target immunochromatography, two sets of color separation amplification schemes and AI imaging analysis, the application improves the recognition ability of bladder tumor specific epitopes, the image quality and the image analysis accuracy in the detection of urinary exfoliative cells.

The invention relates to a kit and a method for detecting phosphorylated Tau protein. In particular to a p-Tau217 protein detection kit and a p-Tau217 protein detection method. The kit and the method disclosed by the invention are based on the p-Tau217 antibody and the total-Tau protein antibody, and are combined with a TSA technology, so that the specificity, the sensitivity and the accuracy of a detection result are improved, and background signals are effectively reduced.

The application discloses a kind of high adhesion enzymatic hydrogel for promoting vascular intimal repair and a preparation method thereof, and belongs to the technical field of hydrogel preparation.The preparation method is as follows: S1, using methacrylic anhydride to modify the double bond of hyaluronic acid, then crosslinking with dopamine to obtain dopamine-modified double-bonded hyaluronic acid;S2, after removing oxygen from the hyaluronic acid solution, add a crosslinking agent and tyramine hydrochloride, and react in an acidic environment to obtain tyramine-modified hyaluronic acid;S3, mix the dopamine-modified double-bonded hyaluronic acid, tyramine-modified hyaluronic acid and deionized water to obtain a hydrogel precursor solution;S4, then use enzymatic reaction to obtain the hydrogel.The method designed by the application is simple, does not require additional light curing equipment, and the constructed hydrogel is similar to the extracellular matrix.The hydrogel can be coated into different shapes to adapt to different environments.The hydrogel is formed by enzymatic reaction, which is rapid, well adhered to the tissue, biocompatible, and suitable for application in vascular repair.

The invention discloses a method for rapidly identifying harmful components in pickled peppers, and belongs to the technical field of food detection. The method comprises the following steps: preparing a reference substance solution, namely preparing a standard substance stock solution and a biogenic amine mixed solution from putrescine, histamine, tyramine and cadaverine standard substances; preparing a test sample solution: preparing the test sample solution by adopting a test sample; the chromatographic conditions are as follows: C18 is used as a filling agent; carrying out gradient elution by taking isopropanol as a mobile phase A and 0.5-2wt% ammonia water as a mobile phase B; the detection wavelength is 254 nm; the column temperature is 25-35 DEG C; and the flow velocity is 0.2-1.0 ml / min. The method has the advantages that (1) the detection speed is high, and the gradient elution speed is high and is only 12 minutes; (2) the number of obvious miscellaneous peaks is small, and only one miscellaneous peak exists; and (3) the method has relatively high precision, relatively low detection limit and good reproducibility.

The invention discloses photosensitive tyramine-sulfonated hyaluronic acid microspheres and a preparation method and application thereof.The preparation method comprises the following steps that 1, S-HA is dissolved in water to prepare a solution, and MES, NHS and EDC are added into the solution; stirring the mixture for 0.5-1.5 hours, adding Tyr into the solution, placing the solution at room temperature for 12-48 hours, adjusting the pH value to 6-8, stirring for 12-48 hours, dialyzing the solution, and freeze-drying the dialyzed solution; and (2) dissolving the S-HA-Tyr prepared in the step (1) in PBS to obtain an S-HA-Tyr solution as a prepolymer solution, adding a photoinitiator into the prepolymer solution, carrying out ultrasonic homogenization to obtain a prepolymer solution containing the photoinitiator, and generating the S-HA-tyr microspheres by using a microfluidic technology. Photosensitive tyramine-sulfonated hyaluronic acid microspheres with uniform particle size are prepared by using a microfluidic technology, the microspheres are successfully grafted on the surface of a magnesium alloy material by using the adhesion of dopamine, and the coating enhances the endothelialization, anti-hyperplasia, anti-inflammatory and anti-coagulation effects of the magnesium alloy stent.

The invention discloses a multi-copper oxidase mutant sourced from Psychrobacter sp. And capable of efficiently degrading biogenic amine and application of the multi-copper oxidase mutant. According to the multi-copper oxidase mutant, the 183rd site of an amino acid sequence shown in SEQ ID NO.1 is mutated into histidine from phenylalanine, the 221st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into aspartic acid from asparagine, the 261st site of the amino acid sequence shown in SEQ ID NO.1 is mutated into glutamic acid from glutamine, and the 292nd site of the amino acid sequence shown in SEQ ID NO.2 is mutated into arginine from lysine. Compared with wild type multi-copper oxidase, the multi-copper oxidase mutant disclosed by the invention has a good degradation effect on tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine, and the degradation rate on the spermidine and the spermine is increased to 100%, so that the multi-copper oxidase mutant has a good application prospect in food fermentation safety production.

The invention discloses an intestinal tract hemostatic composition as well as a preparation method and application thereof, and relates to the field of hemostatic drugs. Wherein the catechin, the hyperoside, the isoquercitrin, the quercetin-3-O-glucuronide, the gallocatechin, the myricetin-3-O-galactoside, the proanthocyanidin B1, the proanthocyanidin B3, the syringe-3-O-glucoside and the astragalus smicus glycoside are flavonoid compounds; nuciferine, higenamine, N-methyl-higenamine and N-trans-feruloyl tyramine are taken as alkaloid compounds, and the compound is selected from the group consisting of higenamine, nuciferine, higenamine The composition has the advantages of simple preparation process, good hemostatic effect and the like, and provides a new raw material choice for the development of intestinal bleeding treatment medicines.

The present application relates to compounds of formula (Ia) or (Ib): or a salt, solvate, or tautomer thereof; wherein R1, and R2 are independently selected from optionally substituted C1 to C 8 alkyl; or R1 and R2 together with the silicon atom to which they are attached form an optionally substituted 5- or 6-membered ring; R3, and R4 are independently selected from H, optionally substituted C1 to C 8 alkyl; optionally substituted C1 to C 8 haloalkyl; or R3 and R4 together with the nitrogen atom to which they are attached form an optionally substituted azetidine or pyrrolidine ring; R3', and R4' are independently selected from H, optionally substituted C1 to C8 alkyl; optionally substituted C1 to C 8 haloalkyl; or R3' and R4' together with the nitrogen atom to which they are attached form an optionally substituted azetidine or pyrrolidine ring;R5 is selected from a negative charge, H, C1 to C 8 alkyl, or optionally substituted aryl; a is 0 or 1; and Z is a divalent linker group; and to fluorophore compositions comprising such compounds, to assay methods and to kits using such fluorophore compositions.

Disclosed herein are recombinant host cells and methods capable of producing tyramine-containing hydroxycinnamic amide compounds, derivatives, and extracts.SOLUTION: Some embodiments provided include, for example, consumable products containing tyramine-containing hydroxycinnamic acid amides produced by recombinant host cells. Some embodiments provided herein are methods for producing tyramine-containing hydroxycinnamic amide or hydroxycinnamic amide derivatives.SELECTED DRAWING: None

The present invention relates to a kit suitable for preparing a biocompatible polymer hydrogel, the kit comprising at least two containers, each container comprising an aqueous composition comprising at least one of tyramine functionalized dextran (Dex-TA), tyramine functionalized hyaluronic acid (HA-TA), a peroxidase and hydrogen peroxide, the present invention relates to a biocompatible polymer hydrogel comprising Dex-TA and HA-TA, which components when mixed are capable of forming said biocompatible polymer hydrogel wherein peroxidase and hydrogen peroxide are each present in different containers wherein the total weight ratio of Dex-TA to HA-TA is between 75: 25 and 25: 75, preferably between 60: 40 and 40: 60, more preferably between 55: 45 and 45: 55; wherein the concentration of Dex-TA is between 2 wt% and 7 wt% relative to the total weight of the aqueous composition in the kit; wherein the concentration of HA-TA varies between 2 wt% and 7 wt% relative to the total weight of the aqueous composition in the kit; wherein Dex-TA comprises unsubstituted dextran repeat units and TA substituted dextran repeat units, and the amount of TA substituted repeat units (DSDEX) in DEX-TA is between 5 mol% and 20 mol% relative to the total number of unsubstituted repeat units and TA substituted repeat units according to proton nuclear magnetic resonance; wherein the HA-TA comprises unsubstituted hyaluronic acid repeat units and TA-substituted hyaluronic acid repeat units, and the amount of TA-substituted repeat units (DSHA) in the HA-TA is between 5 mol% and 20 mol% according to proton nuclear magnetic resonance; wherein the Dex-TA has a weight average molecular weight (Mw) of between 15 kDa and 650 kDa; wherein the HA-TA has a weight average molecular weight (Mw) of between 15 kDa and 550 kDa; wherein the peroxidase is present at a concentration of between 0.25 unit / ml and 10 unit / ml and the hydrogen peroxide is present at a concentration of between 0.01 wt% and 0.10 wt% relative to the total weight of the aqueous composition in the kit.

The application discloses a coumarin tyramine glycoside compound and application thereof in preparation of PTP1B inhibitors and anti-diabetic drugs, and a structural formula of the compound is shown in the following formula: wherein R represents a fatty (aromatic) acyl or a monosaccharide substituent, and R' represents a hydrogen atom or an acetyl group; the compound is prepared by taking 4-methoxycoumarin tyramine 4'-acetyl-O-L-rhamnose as a starting material, introducing a fatty (aromatic) acyl or a monosaccharide substituent into a 2-position hydroxyl and a 3-position hydroxyl of L-rhamnose at the same time, and then removing a protecting group of a sugar group. Pharmacological activity tests show that the compound has good inhibitory activity on protein tyrosine phosphatase 1B, and it is indicated that the coumarin tyramine glycoside compound can be researched and utilized as a new type of PTP1B inhibitor and anti-diabetic drug.

Tyramine-substituted silk fibroin compositions as well as methods for making and using the same are provided.

The application discloses a preparation method and application of an amphiphilic tyrosinase copper competitive binding agent. Two new compounds P4TA or P2TA are synthesized by coupling tyramine TA with 4,4',4'',4'''-(porphyrin-5,10,15,20-tetrayl) tetrakisbenzoic acid TCPP or 3,7,12,17-tetramethyl-8,13-diethyl-2,18-porphyrin dipropionic acid PPIX. An optimal synthesis process of P4TA and P2TA is provided, and an experience is provided for related synthesis. The obtained P4TA and P2TA can recognize tyrosinase and are good copper ion binding reagents, and the two compounds exhibit a significant inhibitory effect on TYR. The application can be used for tyrosinase targeted inhibition and copper ion metabolism intervention in biological medicines and food.

The invention relates to a method for screening and quantitatively determining multiple active ingredients in health food, comprising the following steps: S1: preparing a standard reserve solution; S2: preparing a standard intermediate solution; S3: preparing a standard working solution; S4: sample processing; S5: liquid chromatography quadrupole-electrostatic field orbital trap high-resolution mass spectrometer analysis; S6: ultra-high performance liquid chromatography-quadrupole mass spectrometry analysis. The invention uses high-resolution technology to quickly screen the chemical components in the health food, and simultaneously uses ultra-high performance liquid chromatography-quadrupole mass spectrometry (UPLC-MS / MS) to quantitatively analyze three components of N-p-trans-coumaryltyramide, notoginseng alcohol and notoginseng saponin R2 (S type) in the health food.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

The invention is applicable to the technical field of microorganisms, and provides lactobacillus brevis DF-19 with high yield of tyramine and application of the lactobacillus brevis DF-19, the lactobacillus brevis DF-19 is preserved in China General Microbiological Culture Collection Center on May 26, 2025, and the preservation number is CGMCC No.33896. The strain DF-19 screened and separated by the embodiment of the invention has efficient tyramine production capacity, is easy to culture, has strong acid and salt resistance, specific metabolism, stable heredity and strong biological safety, and provides a basis for efficient production of tyramine and application and development of related products.

The invention is directed to a tissue-adhesive multi-arm polymer comprising a core from which polymeric arms extent, which polymeric arms are substituted with a hydroxyl-substituted aromatic group based on compounds such as dopamine, L-DOPA, D-DOPA, tyramine, noradrenaline and / or serotonin. In addition, the invention is directed to a caprolactam blocked hydroxyl-substituted aromatic compound, suitable for the preparation of the tissue-adhesive multi-arm polymer and to the method for the preparation of the tissue-adhesive multi-arm polymer.

The present disclosure presents hydrogel compositions for use in the treatment of an ocular disorder (e.g., a retinal disease) and methods of treating an ocular disorder in a subject in need thereof with the hydrogel compositions. The hydrogel compositions can include hydroxyphenylpropionic acid (gelatin-HPA), hyaluronic acid-tyramine (HA-Tyr), a catalyzer (e.g., horseradish peroxidase (HRP)), a cell, a crosslinker (e.g., hydrogen peroxide), or any combination thereof. The methods include administering a therapeutically effective amount of a composition into an eye of the subject, wherein the composition includes any of the hydrogel compositions of the disclosure.