Method for preparing fibroin/hyaluronic acid composite external application dressing through enzyme method

A technology of hyaluronic acid and enzymatic preparation, which is applied in the field of textile biology, can solve the problems of cross-linking agent residue easily, affecting the biocompatibility of materials, etc., and achieve good compression mechanical properties and water absorption, mechanical properties improvement, enzyme The effect of high catalytic efficiency

Inactive Publication Date: 2019-12-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While improving the properties of silk fibroin materials, this method also has certain shortcomings, such as crosslinking agents are prone to residues, affecting the biocompatibility of materials, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Hyaluronic acid molecule surface grafted with tyramine: hyaluronic acid, tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide The amine reacts in the same bath. After the reaction, the above mixed solution is dialyzed with deionized water for 24 hours to remove 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide and unreacted tyramine, and then freeze-dried at -50°C to obtain modified hyaluronic acid;

[0023] (2) Laccase catalyzes the cross-linking of silk fibroin and hyaluronic acid and material forming: add laccase to the mixed solution of hyaluronic acid and silk fibroin after step (1), pour it into a polytetrafluoroethylene mold Carry out the reaction; after the reaction, let it stand for 12 hours at 20°C and 60% relative humidity to complete the material molding;

[0024] In step (1), the reaction recipe consists of: 15 g / L of hyaluronic acid with a molecular weight of 10,000, 5 g / L of ty...

Embodiment 2

[0032] (1) Hyaluronic acid molecule surface grafted with tyramine: hyaluronic acid, tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide The amine reacts in the same bath. After the reaction, the above mixed solution is dialyzed with deionized water for 24 hours to remove 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide and unreacted tyramine, and then freeze-dried at -50°C to obtain modified hyaluronic acid;

[0033] (2) Laccase catalyzes the cross-linking of silk fibroin and hyaluronic acid and material forming: add laccase to the mixed solution of hyaluronic acid and silk fibroin after step (1), pour it into a polytetrafluoroethylene mold Carry out the reaction; after the reaction, let it stand for 24 hours at 25°C and a relative humidity of 65% to complete the material molding;

[0034] In step (1), the reaction recipe consists of: 20 g / L of hyaluronic acid with a molecular weight of 100,000, 20 g / ...

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Abstract

The invention discloses a method for preparing fibroin / hyaluronic acid composite external application dressing through enzyme method The method comprises the following steps: modifying hyaluronic acidmolecules with tyramine to increase the number of phenolic hydroxyl groups in the hyaluronic acid molecules and improve the reactivity of hyaluronic acid; and catalyzing crosslinking of silk fibroinand the modified hyaluronic acid with laccase to prepare a hydrogel-based composite external application dressing; and the method specifically comprises the following steps: (1) grafting tyramine on the surfaces of hyaluronic acid molecules; and (2) catalyzing crosslinking of fibroin and hyaluronic acid laccase, and molding an obtained material. Compared with preparation of a fibroin / hyaluronic acid composite external application dressing through a blending method or a chemical crosslinking method, the method has the advantages of being high in enzyme catalysis efficiency, mild in reaction condition and obvious in material mechanical property improvement.

Description

technical field [0001] The invention relates to a method for preparing silk fibroin / hyaluronic acid composite external application material by enzymatic method, which belongs to the field of textile biotechnology. Background technique [0002] Silk fibroin has good biocompatibility, and the silk fibroin peptide and silk film processed from it are widely used in the construction of daily chemical products and medical tissue engineering materials. In addition, hyaluronic acid is an acidic polysaccharide. With its unique molecular structure and physical and chemical properties, it shows a variety of important physiological functions in the body, such as regulating the permeability of blood vessel walls, regulating the diffusion and operation of water and electrolytes, and promoting wound healing. Healing, etc., can be used in the preparation of various hydrogel products. [0003] Hydrogel is a kind of hydrophilic three-dimensional network structure material, which can absorb a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00C08L5/08
CPCC08J3/246C08J2305/08C08J2389/00C08J2405/08C08J2489/00
Inventor 王平王琳周舟余圆圆范雪荣
Owner JIANGNAN UNIV
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