Method for preparing fibroin/hyaluronic acid composite external application dressing through enzyme method
A technology of hyaluronic acid and enzymatic preparation, which is applied in the field of textile biology, can solve the problems of cross-linking agent residue easily, affecting the biocompatibility of materials, etc., and achieve good compression mechanical properties and water absorption, mechanical properties improvement, enzyme The effect of high catalytic efficiency
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Embodiment 1
[0022] (1) Hyaluronic acid molecule surface grafted with tyramine: hyaluronic acid, tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide The amine reacts in the same bath. After the reaction, the above mixed solution is dialyzed with deionized water for 24 hours to remove 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide and unreacted tyramine, and then freeze-dried at -50°C to obtain modified hyaluronic acid;
[0023] (2) Laccase catalyzes the cross-linking of silk fibroin and hyaluronic acid and material forming: add laccase to the mixed solution of hyaluronic acid and silk fibroin after step (1), pour it into a polytetrafluoroethylene mold Carry out the reaction; after the reaction, let it stand for 12 hours at 20°C and 60% relative humidity to complete the material molding;
[0024] In step (1), the reaction recipe consists of: 15 g / L of hyaluronic acid with a molecular weight of 10,000, 5 g / L of ty...
Embodiment 2
[0032] (1) Hyaluronic acid molecule surface grafted with tyramine: hyaluronic acid, tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide The amine reacts in the same bath. After the reaction, the above mixed solution is dialyzed with deionized water for 24 hours to remove 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide and unreacted tyramine, and then freeze-dried at -50°C to obtain modified hyaluronic acid;
[0033] (2) Laccase catalyzes the cross-linking of silk fibroin and hyaluronic acid and material forming: add laccase to the mixed solution of hyaluronic acid and silk fibroin after step (1), pour it into a polytetrafluoroethylene mold Carry out the reaction; after the reaction, let it stand for 24 hours at 25°C and a relative humidity of 65% to complete the material molding;
[0034] In step (1), the reaction recipe consists of: 20 g / L of hyaluronic acid with a molecular weight of 100,000, 20 g / ...
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