Method for utilizing ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of ultra-efficient confluence and biogenic amines
Active Publication Date: 2016-08-17
ANHUI GUJING DISTILLERY +1
View PDF1 Cites 16 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Among them, the colorimetric method can only be semi-quantitative, the liquid chromatography-derivatization method is unstable, the operation is time-consuming and troublesome, and the derivatization reagent benzoyl chloride is toxic, which is not conducive to human health
It has been reported in the literature that the tandem quadrupole method is used to test biogenic amines
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0050] Below in conjunction with example the present invention is described further.
[0051] The present embodiment measures 7 kinds of biogenic amines in a liquor according to the following steps:
[0052] The instruments used are: ultra-high performance convergence chromatograph (equipped with QDa detector); BEH C 18 Composite chromatographic column (3.0×100mm, 1.7μm); Vortex instrument; Pipette gun.
[0053] The specific steps are:
[0054] (1) Pretreatment of the liquor sample to be tested:
[0055] Accurately measure 1.0 mL of the liquor sample to be tested in a 10 mL plastic centrifuge tube, add 9 mL of acetonitrile solution with a volume concentration of 80%, vortex for 5 min, centrifuge at 1000 rpm for 10 min, filter with a 0.22 μm filter membrane needle filter, and obtain the Sample to be tested; sample to be injected;
[0056] (2) Condition setting
[0057] The conditions for setting the ultra-high performance convergence chromatograph are:
[0058] The chromato...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for utilizing an ultra-effective bonded phase chromatography to serially connect QDa while quickly detecting seven biogenic amines in white spirit and belongs to the technical field of detection. The method is characterized by the following steps: after extracting a to-be-detected wine sample with acetonitrile, taking BEH C18 bonded phase chromatographic column as a separating column and carbon dioxide (A)+ isopropanol (B) as flowing phase for performing gradient eluting, and adopting a QDa mass spectrometry detector for detecting after separating the sample through the ultra-effective bonded phase chromatography (UPC2). The method provided by the invention is simple, quick, accurate, reliable and applicable to the simultaneous detection for the contents of seven biogenic amines (histamine, putrescine, cadaverine, phenylethylamine, spermidine, tyramine and octopamine) in white spirit.
Description
technical field [0001] The invention relates to the technical field of detection, in particular to a method for simultaneously rapidly detecting seven biogenic amines in liquor by using ultra-high-efficiency convergence chromatography connected in series with a QDa detector, and belongs to the technical field of wine analysis. Background technique [0002] Biogenic amines are biologically active substances, mainly including β-phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine, etc., which exist in a variety of foods, especially fermented foods (such as cheese, wine, etc.) , beer, rice wine, fermented sausage, condiments), aquatic products and meat products, etc. Biogenic amines, especially histamine, are inherently toxic, so they are a major indicator to measure the freshness of meat and aquatic products and the hygiene conditions in the wine production process. [0003] The current GB standard is detected by colorimetric method or liquid ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more