Liquid chromatography tandem mass spectrometry detection method for catecholamine and metabolite thereof based on magnetic solid-phase extraction

Abstract

The invention discloses a liquid chromatography-tandem mass spectrometry detection method for catecholamine and metabolites thereof based on magnetic solid-phase extraction, which comprises the following steps: 1) preparing a detection sample; 2) constructing a standard curve of a target object; 3) detecting by adopting a liquid chromatography-tandem mass spectrometry method, and calculating the content of catecholamine and metabolites thereof in the detection sample by combining the constructed standard curve. Wherein the catecholamine comprises dopamine, noradrenaline and epinephrine, and metabolites of the catecholamine comprise methoxytyramine, epinephrine and noradrenaline. According to the liquid chromatography-tandem mass spectrometry detection method for catecholamine and metabolite thereof based on magnetic solid-phase extraction provided by the invention, a detection sample of catecholamine and metabolite thereof is pretreated by virtue of a magnetic solid-phase extraction technology, and separation and transfer of a to-be-detected object can be rapidly realized by applying an external magnetic field, so that automatic pretreatment is conveniently realized; meanwhile, the advantages of high selectivity, high purification rate and the like of solid-phase extraction are also reserved.

Description

technical field [0001] The invention relates to the field of clinical mass spectrometry in vitro diagnostic reagents, in particular to a liquid chromatography tandem mass spectrometry detection method for catecholamines and their metabolites based on magnetic solid phase extraction. Background technique [0002] Catecholamines are synthesized and secreted by the adrenal medulla, sympathetic nerves, and paraganglionic chromaffin cells. They are both hormones and neurotransmitters. Catecholamines mainly include dopamine (DA), norepinephrine (NE) and epinephrine (E). Catecholamine oxygen methyltransferase converts DA, E, and NE into methoxytyramine (3-MT), Epinephrine (MN), Norepinephrine (NMN). Catecholamines are endogenous substances with strong physiological effects in organisms, and play an important role in brain and nerve signal transduction. The level of catecholamines in organisms is closely related to various physiological and pathological phenomena. Catecholamines ...

AI Technical Summary

Problems solved by technology

This method of sample pretreatment requires a lot of manual participation, which greatly reduces the efficiency of the experiment. At the same time, because catecholamines and their metabolites are strong polar compounds, the retention ability on the F5 column is very weak. Only the target is retained, and a high proportion of aqueous phase will reduce the ionization efficiency of the mass spectrometer. At the same time, a large number of weakly retained endogenous interference components will co-elute, causing matrix interference to the quantification of the target.

At present, the ion-exchange solid-phase extraction method commonly used in the detection of catecholamines and their metabolites uses a solid-phase extraction 96-well plate that is a high-cost experimental consumable. In the experiment, the solid-phase extraction filler will swell after contacting the organic reagent, which will affect the solution through the solid. The flow rate of the phase extraction packing, resulting in poor well-to-well uniformity

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