18results about How to "Improve Quantitative Accuracy" patented technology

The invention discloses a non-derivatization quantitative detection method for amino acid in a high-concentration protein preparation, which comprises the following steps: separating by using a graphitized carbon chromatographic column, adding an ion pair reagent trifluoroacetic acid into a mobile phase, and detecting by using an electric fog detector (CAD); the method comprises the following steps: preparing a plurality of groups of amino acid standard substance solutions with different concentration groups, wherein the concentration range is 1mmol / L-20mmol / L; drawing a quadratic equation standard curve of the amino acid standard product concentration and the peak area as a detection equation for detecting the amino acid concentration; the method comprises the following steps: diluting a test sample by using ultrapure water for multiple times, carrying out ultrafiltration centrifugal treatment, removing protein, introducing the sample, obtaining a corresponding peak area according to the spectrum integral of the test sample, and substituting the peak area into the detection equation to obtain the concentration of amino acid in the test sample. The method has the advantages of high detection precision, high detection efficiency and simple operation, and greatly reduces the adverse effect of high-concentration protein on the chromatographic column.

This invention discloses a microfluidic chip and a quantitative extraction method for trace liquids. The device includes a quantitative unit, an auxiliary quantitative unit, and an extraction and transport unit. The quantitative unit can be selectively connected to either the auxiliary quantitative unit or the extraction and transport unit. When the quantitative unit is connected to the auxiliary quantitative unit, it is connected to a second microfluidic channel via a first microchannel, allowing the quantitative chamber and the equilibrium chamber to communicate. A portion of the liquid injected through the injection port fills the quantitative chamber for quantitative liquid extraction, while excess liquid flows to the equilibrium chamber for storage. When the quantitative unit is connected to the extraction and transport unit, it is connected to a third microfluidic channel via the first microchannel, allowing the quantitative liquid in the quantitative chamber to be extracted from the outlet. This invention achieves quantitative extraction of trace liquids while reducing the requirements for liquid driving precision, thus improving quantitative accuracy and ease of operation.

The invention discloses a D-protein large-scale screening method based on a thermal proteomics technology, which comprises the following steps: step 1, respectively adding a protein treating agent or not adding the protein treating agent into cells to obtain a D-protein up-regulation group and a D-protein blank group; step 2, obtaining a thermal stability differential protein list by using a thermal proteomics technology; step 3, acquiring a stereoisomeric protein list by utilizing a heterogeneous retrieval technology; step 4, comparing and analyzing the heat stability differential protein list and the stereoisomeric protein list to obtain potential D-protein; and step 5, based on an APM enzyme digestion technology, obtaining the D site of the potential D-protein. The large-scale identification method of the D-protein with high throughput and high coverage is realized through a thermal proteomics technology, the D-amino acid site can be accurately positioned, and the method has important significance on exploration of disease biomarkers, exploration of pathogenesis of major diseases and the like.

The invention provides a high-flux monosaccharide separation and quantification method and kit based on gel electrophoresis. The separation and quantification method comprises the following steps: (1) providing a fluorescence-labeled monosaccharide sample to be detected; (2) injecting the monosaccharide sample to be detected into a capillary electrophoresis system, and performing first electrophoretic separation under a first separation system to obtain a first electrophoretogram; the first separation system comprises borate-free first separation gel and a first buffer solution; (3) cleaning a capillary tube of the capillary electrophoresis system, injecting the monosaccharide sample to be detected into the capillary tube again, and performing electrophoretic separation for the second time in a second separation system to obtain a second electrophoretogram; the second separation system comprises second separation gel containing borate and a second buffer solution; and (4) carrying out qualitative identification and quantitative calculation on the mixed monosaccharide according to the migration time difference and peak overlapping condition of each monosaccharide component in the first electrophoretogram and the second electrophoretogram.

The invention discloses a plant polyphenol extraction detection method based on synchronization of liquid chromatography and tandem mass spectrometry, and relates to the technical field of analytical chemistry detection, and the method comprises the following steps: predicting a chromatographic elution window through an ultraviolet spectrum, calculating a dynamic synchronization index by fusing mass spectrum background noise data, and starting a synchronous extraction detection program according to the dynamic synchronization index. In the extraction process, the mass spectrum scanning frequency and the ion trap filling time are fed back and adjusted according to pressure and temperature data monitored in real time; and when a target chromatographic peak appears, triggering mass spectrum monitoring channel switching and recording fragment ion information. And finally, integrating the fragment ion abundance ratio and a chromatographic peak shape parameter to carry out result verification. According to the method, dynamic coordination of extraction, separation and detection links is realized, and the signal-to-noise ratio, the sensitivity and the method adaptability of polyphenol compound detection in a complex sample are improved.

The invention is suitable for the technical field of magnetic resonance chemical exchange saturation transfer imaging, and provides a magnetic resonance chemical exchange saturation transfer imaging method, device, equipment, product and storage medium. A gradient echo sequence is adopted to collect saturated water molecule signals, and the collected water molecule signals are stored in a K space; and wave control coding is introduced in the acquisition process, and a CEST image is reconstructed based on a water molecule signal of a K space. The introduction of wave control coding can reduce the scanning time. Due to the fact that the wave control coding sampling efficiency is high, a larger matrix size can be supported within the same time, high-resolution imaging is achieved, the partial volume effect can be relieved, and CEST quantitative accuracy is improved.

The utility model relates to quantitative conveying device technical field, and disclose a kind of quantitative conveying device of plastic raw material, including conveying mechanism, mounting bracket, raw material bin, discharge port, reciprocating displacement mechanism, material receiving bin, anti-overflow plate, blanking hole and discharge valve.Using this structure, by reciprocating displacement mechanism drive multiple material receiving bin moves simultaneously, so that any material receiving bin opens to reach material receiving under discharge port after horizontal displacement;Because it is provided with anti-overflow plate, when reciprocating displacement mechanism drives full material receiving bin to continue to move, anti-overflow plate blocks the upper inlet of material receiving bin in a certain range, prevent plastic raw material overflow, while making the plastic raw material of raw material bin unable to continue to fall, realize the full quantitative of material receiving bin;When next empty material receiving bin reaches the blanking hole under raw material bin, continue to receive material, while the discharge valve of previous material receiving bin is opened, and plastic raw material is dropped on conveying mechanism, realize segment quantitative feeding, improve conveying efficiency and quantitative accuracy.