Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry

A catecholamine and high performance liquid chromatography technology, which is applied in the field of liquid chromatography tandem mass spectrometry detection of plasma catecholamines and their metabolites, can solve the problems of high requirements for detection methods and instruments, high difficulty, unfavorable clinical promotion, and the like, and achieves a simple detection process. Fast, enhanced system tolerance, reduced system contamination effect

Pending Publication Date: 2020-05-19
北京豪思生物科技股份有限公司
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Problems solved by technology

[0005] In summary, the main defects of the detection process of catecholamines in the prior art are: 1. The content of catecholamines in plasma is low, which requires high detection methods and instruments; Column retention is difficult, and derivatization method

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  • Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry
  • Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry
  • Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry

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[0025] 1. In order to better illustrate the technical features and effects of the present invention, the following uses specific embodiments to describe the present invention in detail, but the present invention is not limited thereto.

[0026] (1) Calibration of the standard solution.

[0027](a) Preparation of standard working solution: Accurately weigh 12.55 mg of 3-methoxytyramine standard substance and place it in a 10 mL volumetric flask, dissolve it with 4 mL of vitamin C aqueous solution with a concentration of 10 mg / mL, and dilute to the mark with methanol to obtain Standard stock solution A, the concentration of its 3-methoxytyramine is 1.03mg / mL, dilute with 50% methanol solution from the standard stock solution A to obtain the standard intermediate solution B, the concentration of its 3-methoxytyramine is 10.3μg / mL; Accurately weigh 8.46 mg of metanephrine standard substance and place it in a 10 mL volumetric flask, dissolve it with 4 mL of vitamin C aqueous solut...

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Abstract

The invention belongs to the technical field of clinical biological sample detection. The invention specifically relates to a technology for detecting catecholamine and metabolites thereof in blood plasma, in particular to a method for detecting catecholamine and metabolites thereof (epinephrine, norepinephrine, dopamine, metanephrine, normetaneprine and 3-methoxytyramine) by high-throughput liquid chromatography-tandem mass spectrometry. The method comprises the steps of standard solution preparation, sample treatment, sample detection and formula calculation. The method can be used for simultaneously detecting six substances including catecholamine and metabolites thereof, and has the advantages of high accuracy, high sensitivity, high specificity, small required sample volume, simple sample pretreatment, good tolerance and the like.

Description

technical field [0001] The invention relates to a detection method of high-throughput liquid chromatography tandem mass spectrometry and a method for detecting catecholamines and metabolites thereof by high-throughput liquid chromatography tandem mass spectrometry. Background technique [0002] Catecholamine is a neurological substance containing catechol and amine groups, including epinephrine (epinephrine, E), norepinephrine (norepinephrine, NE), and dopamine (dopamine, DA), which are produced by the adrenal medulla and some sympathetic neurons. It is a very important neurotransmitter secreted by neuronal chromaffin cells, and it is also an important hormone substance. These three catecholamines are all converted from tyrosine. After the metabolism of catecholamines in the body, they are converted into corresponding metabolites such as methoxyepinephrine (or metanephrine, Metanephrine, MN), methoxyl Norepinephrine (or norepinephrine, Normetaneprine, NMN), 3-methoxytyramin...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/54G01N30/60
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/54G01N30/60G01N2030/045G01N2030/324
Inventor 周洲王恺隽赵丽平武红梅陆开智肖冰心潘媛媛李彩霞何启新倪伟栗琳
Owner 北京豪思生物科技股份有限公司
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