Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry

A catecholamine and high performance liquid chromatography technology, which is applied in the field of liquid chromatography tandem mass spectrometry detection of plasma catecholamines and their metabolites, can solve the problems of high requirements for detection methods and instruments, high difficulty, unfavorable clinical promotion, and the like, and achieves a simple detection process. Fast, enhanced system tolerance, reduced system contamination effect

Pending Publication Date: 2020-05-19
北京豪思生物科技股份有限公司
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, the main defects of the detection process of catecholamines in the prior art are: 1. The content of catecholamines in plasma is low, which requires high detection methods and instruments; Column retention is difficult, and derivatization methods are commonly used for sample pretreatment, but other operations are complicated, which is not conducive to clinical promotion; 3. It is difficult to detect 6 kinds of catecholamines at one time, and most of the currently disclosed methods only detect some of them

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry
  • Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry
  • Method for detecting plasma catecholamine and metabolites thereof by liquid chromatography-tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. In order to better illustrate the technical features and effects of the present invention, the following uses specific embodiments to describe the present invention in detail, but the present invention is not limited thereto.

[0026] (1) Calibration of the standard solution.

[0027](a) Preparation of standard working solution: Accurately weigh 12.55 mg of 3-methoxytyramine standard substance and place it in a 10 mL volumetric flask, dissolve it with 4 mL of vitamin C aqueous solution with a concentration of 10 mg / mL, and dilute to the mark with methanol to obtain Standard stock solution A, the concentration of its 3-methoxytyramine is 1.03mg / mL, dilute with 50% methanol solution from the standard stock solution A to obtain the standard intermediate solution B, the concentration of its 3-methoxytyramine is 10.3μg / mL; Accurately weigh 8.46 mg of metanephrine standard substance and place it in a 10 mL volumetric flask, dissolve it with 4 mL of vitamin C aqueous solut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Column lengthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of clinical biological sample detection. The invention specifically relates to a technology for detecting catecholamine and metabolites thereof in blood plasma, in particular to a method for detecting catecholamine and metabolites thereof (epinephrine, norepinephrine, dopamine, metanephrine, normetaneprine and 3-methoxytyramine) by high-throughput liquid chromatography-tandem mass spectrometry. The method comprises the steps of standard solution preparation, sample treatment, sample detection and formula calculation. The method can be used for simultaneously detecting six substances including catecholamine and metabolites thereof, and has the advantages of high accuracy, high sensitivity, high specificity, small required sample volume, simple sample pretreatment, good tolerance and the like.

Description

technical field [0001] The invention relates to a detection method of high-throughput liquid chromatography tandem mass spectrometry and a method for detecting catecholamines and metabolites thereof by high-throughput liquid chromatography tandem mass spectrometry. Background technique [0002] Catecholamine is a neurological substance containing catechol and amine groups, including epinephrine (epinephrine, E), norepinephrine (norepinephrine, NE), and dopamine (dopamine, DA), which are produced by the adrenal medulla and some sympathetic neurons. It is a very important neurotransmitter secreted by neuronal chromaffin cells, and it is also an important hormone substance. These three catecholamines are all converted from tyrosine. After the metabolism of catecholamines in the body, they are converted into corresponding metabolites such as methoxyepinephrine (or metanephrine, Metanephrine, MN), methoxyl Norepinephrine (or norepinephrine, Normetaneprine, NMN), 3-methoxytyramin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/54G01N30/60
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/54G01N30/60G01N2030/045G01N2030/324
Inventor 周洲王恺隽赵丽平武红梅陆开智肖冰心潘媛媛李彩霞何启新倪伟栗琳
Owner 北京豪思生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com