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Kit for detecting eight catecholamines and metabolites thereof in urine by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

A technology of ultra-high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of urine detection and can solve problems such as high cost and complicated pretreatment

Inactive Publication Date: 2020-07-10
南京品生医学检验实验室有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are basically 3 to 5 types of detection methods for urinary catecholamines and their metabolites, and solid-phase extraction is used for pretreatment, which is not only complicated in pretreatment, but also high in cost.

Method used

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  • Kit for detecting eight catecholamines and metabolites thereof in urine by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Kit for detecting eight catecholamines and metabolites thereof in urine by using ultra-high performance liquid chromatography-tandem mass spectrometry technology
  • Kit for detecting eight catecholamines and metabolites thereof in urine by using ultra-high performance liquid chromatography-tandem mass spectrometry technology

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Experimental program
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Effect test

Embodiment 1

[0135] 1. Experimental materials and instruments

[0136] 1. Material

[0137] The methodological research experiment samples come from urine samples collected in the outpatient clinic of Wuhan Asia Heart Hospital in May 2019.

[0138] (1) Instrument: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with autosampler, Waters Corporation); SCILOGEX D2012 high-speed benchtop centrifuge (USA) ; Ultrapure water instrument (ELGA LabWater, UK); Multi-tube vortex mixer (Vortex genie2, USA); Adjustable pipette (Eppendorf 0.5~10μL, 10~100μL, 100~1000μL); glass instrument, graduated cylinder, etc. .

[0139] (2) Reagent consumables: MS-grade acetonitrile (Fisher, USA); MS-grade formic acid (Merck, USA); Chromatographic column Agilent Pursuit PFP (2.0×150mm, 3.0μm) (Agilent Technology); 0.22μm nylon filter membrane (Bona Ai Agere); 1 mL syringe (Jinta, China).

[0140] (3) Standards: epinephrine, norepinephrine,...

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Abstract

The invention relates to a kit for detecting eight catecholamines and metabolites thereof in urine by using an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The kitcomprises an eluent, a calibrator solution, a mixed internal standard solution, an extract solution and a quality control material, wherein the eight catecholamines and metabolites thereof are respectively epinephrine, norepinephrine, dopamine, metadrenaline, normetanephrine, 3-methoxytyramine, homovanillic acid and vanilmandelic acid. The kit disclosed by the invention is used for detecting eight catecholamines and metabolites thereof in urine, the pretreatment process is simple, solid-phase extraction or derivatization reaction is not needed, the cost is low, the sensitivity is high, the specificity is high, the pretreatment is completed within 10 minutes, the separation and detection of the eight catecholamines and the metabolites thereof are completed within 4.5 minutes, the matrix effect and the precision basically meet the requirements, and the kit can be used for quantitatively analyzing the eight catecholamines and the metabolites thereof clinically.

Description

Technical field [0001] The invention belongs to the technical field of urine detection, and specifically relates to a kit for detecting 8 catecholamines and their metabolites in urine by ultra-high performance liquid chromatography tandem mass spectrometry technology. Background technique [0002] Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) are converted from tyrosine under the action of enzymes. These three catecholamines are all important neurotransmitters in the central nervous system. It plays an important role in self-regulating functions such as vascular tension, intestinal and bronchial smooth muscle tension, heart rate and contractility, and glucose metabolism. 3-Methoxytyramine (3MT), Metanephrine (Metanephrine, MN) and Normetanephrine (Normetanephrine, NMN) are metabolites of dopamine, epinephrine and norepinephrine, respectively. These metabolites are further metabolized into vanillymandelic acid (VMA) and homovanillic acid (HVA). [0003] Pheochromocytoma i...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/38
CPCG01N30/02G01N30/06G01N30/38G01N2030/045
Inventor 成晓亮李美娟
Owner 南京品生医学检验实验室有限公司
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