Screening method of liver cirrhosis cation marker and application

A screening method and positive ion technology, applied in the field of biochemical detection, can solve the problems of high cost, low sensitivity and specificity of ultrasound technology, and limited sample error of biopsy

Active Publication Date: 2020-02-28
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the diagnosis of early liver cirrhosis mainly relies on ultrasound and tissue biopsy. The sensitivity and specificity of ultrasound technology is not very high. including sample error and bias between different readers), prompting the search for an alternative

Method used

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  • Screening method of liver cirrhosis cation marker and application
  • Screening method of liver cirrhosis cation marker and application
  • Screening method of liver cirrhosis cation marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 The screening method for cationic markers of liver cirrhosis according to the present invention comprises the following specific steps:

[0052] The first step is to extract samples: weigh 30 mg of sample tissue (including 40 normal liver tissue samples (normal samples) and 40 liver cirrhosis samples), wash twice with 4 ° C pre-cooled PBS solution, and then add each Add 200 μL of ultrapure water to the samples, homogenize with a homogenizer, vortex for 60 seconds, add 800 μL of methanol and acetonitrile mixture (V / V=1:1), vortex for 60 seconds, and ultrasonically break at low temperature (4°C) for 30 minutes. Repeat the ultrasound twice, incubate at -20°C for 60min to precipitate the protein in the sample solution, centrifuge at 4°C and 14000rcf for 20min with a centrifuge, take the supernatant and freeze-dry to obtain a powdery metabolic mixture (lyophilized powder). The powdered metabolic mixture was frozen and stored at -80°C for later use;

[0053] In the...

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Abstract

The invention discloses a screening method of a liver cirrhosis cation marker. The method comprises the following steps: 1, extracting a sample; 2, separating and collecting mass spectrum data by using LC-MS / MS: separating the metabolic mixture obtained in the step 1 by using the high performance liquid chromatography, detecting and analyzing the separated sample by using a mass spectrometer I, and collecting a primary spectrogram and a secondary spectrogram of the sample by using a mass spectrometer II; 3, determining metabolic substances; 4, screening metabolites with the significant difference. The screening method of the cationic marker related to liver cirrhosis diagnosis is established for the first time; the combination of tyramine cations, 2-phenylacetamide cations, triethanolaminecations and Val-Ile cations obtained through screening and the 2-phenylacetamide cations can be used as new markers for liver cirrhosis diagnosis, new targets and ideas are provided for research anddevelopment of drugs for liver cirrhosis diagnosis in the future, and important significance is achieved.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a method for screening cationic markers of liver cirrhosis, and also relates to the application of the screened cationic markers of liver cirrhosis in the preparation of diagnostic drugs or diagnostic kits for liver cirrhosis. Background technique [0002] Hepatocellular carcinoma (HCC), a primary liver cancer with high mortality, is the most common malignant tumor worldwide, especially in Asia, Africa and southern Europe. The number of new cases worldwide is about 650,000 every year, and its incidence rate ranks fifth among all malignant tumors. The death toll is about 600,000, ranking third among all malignant tumors. [0003] The pathogenesis of liver cancer is various. At present, liver cancer in my country mainly develops on the basis of liver cirrhosis caused by hepatitis B or nonalcoholic steatohepatitis. That is, viral hepatitis and liver cirrhosis are the main patho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N30/02G01N30/36G01N30/06G01N30/34G01N30/72G01N30/86
CPCG01N33/5308G01N30/02G01N30/36G01N30/06G01N30/34G01N30/7266G01N30/8679G01N2030/027G01N2800/085
Inventor 李捷袁梓博庞春孙耀辉李涛杨翰孙校炎火钟坤贺英杰闫鑫
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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