Triptolide-gold nanoparticle/hyaluronic acid composite hydrogel as well as preparation and application thereof

A technology of triptolide and gold nanoparticles, which is applied in the direction of drug combination, wave energy or particle radiation treatment materials, medical preparations of non-effective ingredients, etc., can solve the problems of limited application, large toxic and side effects, and poor solubility. Achieve the effects of avoiding side effects, low toxicity, and inhibiting proliferation

Active Publication Date: 2021-11-02
BEIJING UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
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Problems solved by technology

However, due to poor water solubility of TP, rapid elimination in the body, and severe side effects, it greatly limits its clinical application.

Method used

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  • Triptolide-gold nanoparticle/hyaluronic acid composite hydrogel as well as preparation and application thereof
  • Triptolide-gold nanoparticle/hyaluronic acid composite hydrogel as well as preparation and application thereof
  • Triptolide-gold nanoparticle/hyaluronic acid composite hydrogel as well as preparation and application thereof

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Embodiment 1

[0029] 1. Experimental materials

[0030] Bovine type II collagen (Sigma-Aldrich, Shanghai), incomplete Freund's adjuvant (Sigma-Aldrich, Shanghai), complete Freund's adjuvant (Sigma-Aldrich, Shanghai), mTOR, p-mTOR, p70s6k, p- The primary antibody of p70s6k was purchased from Abcam, goat anti-rabbit, goat anti-mouse secondary antibody was purchased from Zhongshan Jinqiao, 10×SDS-PAGE electrophoresis buffer (B1005-500ML), 10×protein electrotransfer buffer (B1006-500ml) Purchased from Beijing Pulilai Gene Technology Co., Ltd. Tri-MOPS-SDS instant granules, Western and IP cell lysate (P0013-100ML), SDS-PAGE gel preparation kit (P0012A), Quickblock TM Blocking solution (TBSTx) was purchased from Biyuntian Institute of Biotechnology. Blue Plus IVProtein Marker (10-180kDa) (Beijing Quanshijin Biotechnology Co., Ltd.). Carboxypolyethylene glycol mercapto, cystamine dihydrochloride, tyramine hydrochloride (TA), dithiothreitol (DTT), 1-ethyl-(3-dimethylaminopropyl)carbodiimide Hyd...

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Abstract

The invention relates to triptolide-gold nanoparticle/hyaluronic acid composite hydrogel as well as preparation and application thereof. The preparation method comprises the following steps: preparing the TP-Au-RGD nano particles; preparing tyramine and sulfydryl modified hyaluronic acid; and preparing the TP-Au/HA composite hydrogel. The triptolide-gold nanoparticle/hyaluronic acid composite hydrogel designed by the invention has specific targeting on inflammatory joints, not only can reach effective drug concentration locally, but also can reduce the dosage of drugs and inhibit the proliferation of rheumatoid fibroblast-like synovial cells, and is low in toxicity, so that the side effects of systemic administration are avoided. The triptolide-gold nanoparticle/hyaluronic acid composite hydrogel can achieve the same effect with a smaller dose of medicament compared with conventional treatments.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and specifically relates to a triptolide-gold nanoparticle / hyaluronic acid composite hydrogel and its preparation and application. [0002] technical background [0003] Rheumatoid arthritis (Rheumatoid arthritis, RA) is a chronic autoimmune disease with multiple joint inflammation as the main clinical manifestation. High (60%-70%), and the prognosis is poor, seriously endangering human life and health. The etiology of RA is not yet clear, but it is generally believed to be related to various factors such as abnormal immune system, heredity, and infection. The basic pathological features of RA patients include synovitis and angiogenesis. On the one hand, synovial blood vessels proliferate and secrete a large number of cytokines, which cause synovial tissue thickening, destroy cartilage and bone structure integrity, and invade soft tissue around bone joints, resulting in joint damage....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/585A61K41/00A61K47/36A61K47/69A61K47/62A61K47/34A61K47/20A61P19/02A61P29/00C08B37/08
CPCA61K9/06A61K9/0024A61K41/0042A61K31/585A61K47/36A61K47/6937A61K47/6935A61K47/62A61P19/02A61P29/00C08B37/0072A61K2300/00
Inventor 刘元艳李晨曦刘蕊宋钰蓉华正来罗昕怡李阳
Owner BEIJING UNIV OF CHINESE MEDICINE
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