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Method for preparing hyaluronic acid-based conductive film material by enzymatic method

A technology of hyaluronic acid and enzymatic preparation, applied in fermentation and other directions, can solve the problems of poor mechanical properties of membrane materials, decreased electrical conductivity, easy to break, etc., and achieve long-lasting electrical conductivity, improved mechanical properties, and strong specificity. Effect

Active Publication Date: 2019-12-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also certain deficiencies in this type of method, including: (1) the intermolecular binding force between polypyrrole and unmodified hyaluronic acid is weak, and the macromolecules of polypyrrole in the membrane material in the aqueous phase are easily eluted, which impels the electrical conductivity to decline; (2) The unmodified hyaluronic acid molecules mainly rely on hydrogen bonds and van der Waals forces, and the prepared film materials have poor mechanical properties and are prone to fracture during stretching and compression deformation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Hyaluronic acid molecule surface grafted with tyramine: hyaluronic acid, tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide The amine reacts in the same bath; after the reaction, the above mixed solution is dialyzed with deionized water for 24 hours to remove 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide and unreacted tyramine, and then freeze-dried at -50°C to obtain modified hyaluronic acid;

[0021] (2) Laccase catalyzes the cross-linking of hyaluronic acid and pyrrole polymerization: add laccase to the mixed solution of hyaluronic acid and pyrrole after the treatment in step (1), react in a polytetrafluoroethylene mold, and heat , Standing for 10 hours in a relative humidity of 60% to form a membrane material.

[0022] In step (1), the reaction recipe consists of: 25 g / L of hyaluronic acid with a molecular weight of 10,000, 5 g / L of tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodi...

Embodiment 2

[0028] (1) Hyaluronic acid molecule surface grafted with tyramine: hyaluronic acid, tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide The amine reacts in the same bath. After the reaction, the above mixed solution is dialyzed with deionized water for 24 hours to remove 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide and unreacted tyramine, and then freeze-dried at -40°C to obtain modified hyaluronic acid;

[0029] (2) Laccase catalyzes hyaluronic acid crosslinking and pyrrole polymerization: add laccase to the mixed solution of hyaluronic acid and pyrrole after step (1), react in a polytetrafluoroethylene mold, and heat , Standing for 20 hours in a relative humidity of 65% to form a membrane material.

[0030] In step (1), the reaction recipe consists of: 30 g / L of hyaluronic acid with a molecular weight of 100,000, 20 g / L of tyramine, 1-ethyl-(3-dimethylaminopropyl) carbodiimide Hydrochloride 5g / ...

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Abstract

The invention discloses a method for preparing hyaluronic acid-based conductive film material by enzymatic method, which utilizes tyramine to modify hyaluronic acid molecules, increases the number ofphenolic hydroxyl groups in hyaluronic acid molecules, and improves the reactivity of hyaluronic acid; then hyaluronic acid-based conductive film materials are prepared by laccase-catalyzed oxidation-modified hyaluronic acid intermolecular crosslinking and pyrrole polymerization. Specifically, it comprises the following steps: (1) grafting tyramine on the surface of hyaluronic acid molecules; (2)laccase-catalyzed hyaluronic acid crosslinking and polymerization with pyrrole. Compared with the hyaluronic acid-based conductive film material prepared by the blending method or the chemical initiation method, the invention has the advantages of high enzyme catalysis efficiency, mild reaction conditions, and obvious improvement of mechanical properties and conductive properties of the film material.

Description

technical field [0001] The invention relates to a method for enzymatically preparing a hyaluronic acid-based conductive film material, which belongs to the technical field of biological material processing. Background technique [0002] Hydrogel is a kind of hydrophilic three-dimensional network structure material, which can absorb a large number of water molecules and connect them inside the network. It can absorb water equivalent to hundreds of times its own mass and still maintain a gel state. Water-locking, and the hydrogel can well simulate human tissue after absorbing water and swelling, has a certain degree of softness and viscoelasticity, and can be attached to the skin surface. Hyaluronic acid is an acidic polysaccharide. With its unique molecular structure and physical and chemical properties, it shows a variety of important physiological functions in the body, such as regulating the permeability of blood vessel walls, regulating the diffusion and operation of wate...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08L79/04C08J5/18C08J3/24C08B37/08C12P17/10
CPCC08B37/0072C08J3/246C08J5/18C08J2305/08C08J2379/04C12P17/10
Inventor 王平农叶琳王琳袁久刚王强
Owner JIANGNAN UNIV
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