Method and kit for detecting antiasthmatic drugs in serum

A technology for anti-asthma and drugs, which is applied in the field of detection of anti-asthma drugs in serum, can solve the problems of good linearity, high feasibility, low detection signal, and low sensitivity, and achieve the goal of reducing time detection cost, less reagent consumption, and good extraction effect Effect

Inactive Publication Date: 2021-06-29
JINAN YING SHENG BIOTECH
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  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to improve the deficiencies of the prior art, the application provides a method and kit for detecting anti-asthmatic drugs in serum, the anti-asthmatic drugs are theophylline, diprophylline and doxofylline, the method of the present invention It can effectively shorten the detection time while improving the resolution, improve the efficiency of analysis and measurement, and solve the problems of low detection signal and low sensitivity; meanwhile, the method of the present invention has good linearity and high feasibility

Method used

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  • Method and kit for detecting antiasthmatic drugs in serum
  • Method and kit for detecting antiasthmatic drugs in serum
  • Method and kit for detecting antiasthmatic drugs in serum

Examples

Experimental program
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Effect test

Embodiment 1

[0058] 1. Extract theophylline, dyphylline and doxofylline in serum

[0059] (1) Add 25 μL serum sample to the centrifuge tube, add 100 μL extract solution, the extract solution is a mixture of methanol, acetonitrile and trichloroacetic acid solution, the volume ratio of methanol and acetonitrile is 1:5 (the volume fraction of trichloroacetic acid is 0.05 %).

[0060] (2) The above-mentioned sample solution was vortexed for 5 minutes, and the speed of the centrifuge was 12000 r / min; centrifuged for 5 minutes.

[0061] (3) After centrifugation, take 10 μL of supernatant to a 96-well receiving plate, add 100 μL of diluent, and mix well. The composition of the diluent is an aqueous solution containing 0.05% trichloroacetic acid by volume.

[0062] (4) Cover with an aluminum foil sealing film for chromatographic analysis.

[0063] 2. Instrument method

[0064] (1) Chromatographic conditions: chromatographic column: ZORBAX SB C18 (2.1mm×100mm, 3.5μm), column temperature: 40°C; m...

Embodiment 2

[0100] Embodiment 2 standard curve linear analysis

[0101] Add mixed standard to bovine serum, the concentration of theophylline, diprophylline and doxofylline are 20 μg / mL, 10 μg / mL, 10 μg / mL in sequence. Using bovine serum as the diluent, the bovine serum standard mixture was sequentially diluted 1:1 in equal proportions, with a total of 6 concentration gradients, and the method in Example 1 was used for detection.

[0102] Use data processing software to data processing, calculate and draw standard curve, wherein, the standard curve figure of theophylline, diprophylline, doxofylline is as follows Figure 13 , Figure 14 and Figure 15 As shown, the correlation coefficient R2 is greater than 0.999, which meets the linearity requirement.

[0103] Among them, the linear range of theophylline is 0.1-80 μg / mL, and the linear range of diprophylline and doxofylline is 0.05-40 μg / mL.

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Abstract

The invention provides a method and a kit for detecting antiasthmatic drugs in serum. In the method, the antiasthmatic drugs are theophylline, diprophylline and doxofylline. The method comprises the steps that a serum sample is extracted and then detected and analyzed through an HPLC-MS method, and an extracting solution is a mixed solution of methanol containing trichloroacetic acid and acetonitrile. According to the method, the separation degree can be improved, the detection time can be effectively shortened, the analysis and determination efficiency is improved, and the problems of low detection signal and low sensitivity are solved; meanwhile, the method is good in linearity and high in feasibility.

Description

technical field [0001] The application relates to the technical field of detection of blood drug concentration, in particular to a method and a kit for detecting anti-asthmatic drugs in serum. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the application, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Theophylline drugs are mainly used in the treatment of chronic obstructive pulmonary disease and asthma. The most commonly used theophylline drugs in clinical practice include theophylline, aminophylline, doxofylline, cholophylline, dihydroxyprophylline, etc. The therapeutic dose of theophylline drugs is quite close to the toxic dose, the therapeutic index is narrow, and there are many factors affecting the oral absorption of this type of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/72
CPCG01N30/06G01N30/34G01N30/72G01N30/88G01N2030/027G01N2030/8809
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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