Detection method of graphene oxide test paper for identification of collagen peptide

A detection method, graphene technology, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., to achieve the effect of simple operation, convenient detection, and low price

Inactive Publication Date: 2016-12-14
武汉采思生物科技有限公司
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods provide an important platform for understanding the biological roles played by proteins, but they are limited to well-folded proteins
The development of detection methods for unfolded proteins or peptides associated with diseases still faces great challenges

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of graphene oxide test paper for identification of collagen peptide
  • Detection method of graphene oxide test paper for identification of collagen peptide
  • Detection method of graphene oxide test paper for identification of collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Substrate preparation

[0030] Cut the nitrocellulose membrane into a rectangle of 3±0.3mm*1±0.1mm, drop 0.7ul of a certain concentration of graphene oxide solution on the nitrocellulose membrane, dry it at room temperature for 10min, and drop 0.7ul of 20mM PBS buffer solution on the dry on the substrate;

[0031] (2) Probe preparation

[0032] The amino acid sequence is G(PRGPOG) 5 The polypeptide is modified with FITC fluorescent dye and purified by liquid chromatography to obtain the probe polypeptide FITC-G (PRGPOG) 5 ;

[0033] (3) Fluorescence quenching curve

[0034] Drop different concentrations of graphene oxide solutions on the nitrocellulose membrane, dry at room temperature for 10 minutes, drop 0.7ul 20mM PBS buffer solution on the substrate and dry it; drop 0.7ul 15uM probe polypeptide solution into the substrate, and incubate at room temperature 10min and dry; in a dark place, irradiate the test paper with 312nm ultraviolet light for fluorescence ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a detection method of graphene oxide test paper for identification of collagen peptide. The method comprises the following steps: (1) substrate preparation: modifying a nano material capable of fluorescence resonance energy transfer to absorbent paper, cutting the absorbent paper into rectangular of 3+ / - 0.3mm*1+ / - 0.1mm, drying naturally at 20-40 DEG C, dropwise adding 0.5-1 [mu]l 20mM PBS buffer on the substrate and drying naturally; (2) probe preparation: modifying a peptide with specific sequence by fluorescent molecules; (3) probe and substrate hybrid: dropwise adding 0.5-1 ul of a probe peptide solution to the substrate for hybridization, incubating for 10-20min and drying naturally; and (4) identification of collagen peptide: premixing an analyte and probe peptide, dropwise adding 0.5-1 [mu]l of the mixed liquid to the substrate, incubating for 20-40 min, and drying naturally; and exciting by using excitation wavelength of fluorescent molecules in step (2) in the dark, and detecting the degree of fluorescence recovery. The test paper has the advantages of low cost, simple operation, convenient detection, good specificity, and small sample amount.

Description

technical field [0001] The invention relates to a graphene oxide test paper detection method for identifying collagen peptides, belonging to the technical field of biological detection. Background technique [0002] The unfolded fragments produced by collagen degradation are important targets for disease diagnosis and treatment. As the main component of connective tissue, collagen has the function of supporting and protecting soft tissues. Collagenase hydrolysis plays an important role in many biological processes such as wound healing, tissue development and regeneration; however, excessive degradation of collagen will lead to a large number of diseases such as arthritis and tumors. Therefore, the development of antibodies and peptide drugs targeting the degradation products of collagen in the unfolded state has always been a research hotspot. Collagen highly reproducible (Gly-X-Y) n The amino acid sequence makes it very difficult to develop antibodies with high specific...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486G01N21/6402
Inventor 肖建喜蔡向东李婷婷杨秋霞丁嘉敏
Owner 武汉采思生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap