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Instrument-free and power-free rapid on-site nucleic acid testing product

A power-free, nucleic acid technology, applied in biochemical instruments, microbial determination/inspection, bioreactor/fermenter combination, etc., can solve the problems of high cost, no rapid nucleic acid detection, no small and portable convenience, etc.

Inactive Publication Date: 2021-04-09
GUANGZHOU PLUSLIFE TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, nucleic acid detection products based on the above nucleic acid isothermal amplification technology are equipped with a temperature control device. The current temperature control device used for nucleic acid detection is an electric heating temperature control device, which needs to be equipped with a power supply. The structure is complex and the cost is high. , does not have the advantages of small size and portability, simple operation, and convenient use, and does not really realize rapid on-site detection of nucleic acids

Method used

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  • Instrument-free and power-free rapid on-site nucleic acid testing product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 No-instrument-free, power-free, single-reaction result area nucleic acid on-site rapid detection card

[0045] It includes a casing (7), a nucleic acid detection module, and a temperature control module, and the nucleic acid detection module and the temperature control module are fixed inside the casing (7).

[0046] Before use, the test card is stored in an airtight outer package, and the inside of the airtight outer package is evacuated or filled with inert gas to block the reaction.

[0047] The temperature control module includes a self-heating substance in the air, a sealed breathable bag, and a breathable structure (2). The self-heating substance in the air is a mixture of iron powders. The temperature control module is pre-installed with the iron powder mixture and placed inside the sealed breathable bag. The temperature control module is introduced into the outside air through the air-permeable structure (2) during detection, and the iron powder mixtur...

Embodiment 2

[0050] Example 2 Instrument-free and power-free nucleic acid on-site rapid detection card

[0051] It includes a housing (7), a nucleic acid detection module, and a temperature control module. The nucleic acid detection module and the temperature control module are fixed inside the housing (7), and a sample pretreatment device is also included.

[0052] Before use, the test card is stored in an airtight outer package, and the inside of the airtight outer package is evacuated or filled with inert gas to block the reaction.

[0053] The temperature control module includes a self-heating substance in the air, a sealed breathable bag, and a breathable structure (2). The self-heating substance in the air is a mixture of iron powders. The temperature control module is pre-installed with the iron powder mixture and placed inside the sealed breathable bag. The temperature control module is introduced into the outside air through the air-permeable structure (2) during detection, and the ...

Embodiment 3

[0056] Example 3 Exempt from instruments and exempt from multiple power supplies on-site rapid detection card for nucleic acid

[0057] It includes a housing (7), a nucleic acid detection module, and a temperature control module. The nucleic acid detection module and the temperature control module are fixed inside the housing (7), and a sample pretreatment device is also included.

[0058] Before use, the test card is stored in an airtight outer package, and the inside of the airtight outer package is evacuated or filled with inert gas to block the reaction.

[0059] The temperature control module includes a self-heating substance in the air, a sealed breathable bag, and a breathable structure (2). The self-heating substance in the air is a mixture of iron powders. The temperature control module is pre-installed with the iron powder mixture and placed inside the sealed breathable bag. When testing with the temperature control module, outside air is introduced through the breat...

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Abstract

The invention belongs to the technical field of nucleic acid testing, and particularly relates to an instrument-free and power-free rapid on-site nucleic acid testing product. The product comprises a nucleic acid testing module and a temperature control module for providing a nucleic acid testing reaction temperature environment, and is characterized in that the temperature control module includes an air-touch self-heating substance and a ventilation structure; and the temperature control module introduces external air through the ventilation structure during testing. The invention provides the instrument-free and power-free rapid on-site nucleic acid testing product which is simple and convenient to operate, does not depend on large or expensive instruments, is power-free and relatively low in cost, so that nucleic acid testing is realized truly anytime and anywhere, and the application scene of nucleic acid testing is greatly extended.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and in particular relates to an instrument-free and power-free on-site nucleic acid rapid detection product. Background technique [0002] Nucleic acid detection technology, after decades of development and rapid maturity in recent years, has been widely used in the detection of infectious disease pathogens (including bacteria, mycoplasma, viruses, etc.), tumor mutation detection, genetic disease detection, individual gene Type detection and other aspects. In many application fields, such as the diagnosis of infectious diseases, the sensitivity and specificity of nucleic acid detection technology are much better than colloidal gold / immunofluorescence method, and it is the gold standard for various diagnoses. However, almost all current nucleic acid detection technologies rely on instruments, and have high requirements for sample processing, detection environment, and personnel qua...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/34
CPCC12Q1/6844C12Q2521/507C12Q2531/119
Inventor 陈翀刘华勇叶月娥黄嘉恩
Owner GUANGZHOU PLUSLIFE TECH CO LTD
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