Instrument-free nucleic acid spot quick detection reagent kit

Abstract

The invention discloses an instrument-free nucleic acid spot quick detection reagent kit. A detection card comprises a sample inlet opening, a sample treatment module, a puncturing pipe, a reaction liquid cavity, a reaction liquid flow channel, a main reaction region, n second reaction regions and corresponding n driving assemblies, a temperature control region, a result region, a result driving assembly, a waste liquid region, a housing and the like, wherein n is greater than or equal to 0. Through the adoption of the detection card, extracting and purifying a nucleic acid sample in advance are not needed, so that spot detection from raw samples to the result is realized, the operation is simple, convenient, quick and low in mistake rate, two-step operations operated by a nonprofessional person are only needed, mistakes and errors brought by different operators are substantially reduced, and the risk of aerosol pollution is also avoided; requirements for sample treatment, detection environment and staff quality are not high, the pollution risk is low, and the false positive rate is very low; the detection card is independent of large-scale instruments or expensive instruments, is low in cost, can be directly applied to detection of spot complex multiple various nucleic acid samples, is also suitable for various detection reactions having multiple similar complex, is wide in application range, and good in application prospects.

Description

technical field [0001] The invention belongs to the technical field of detection. More specifically, it relates to an instrument-free on-site nucleic acid rapid detection kit. Background technique [0002] Nucleic acid detection technology, after decades of development and rapid maturity in recent years, has been widely used in the detection of infectious disease pathogens (including bacteria, mycoplasma, viruses, etc.), tumor mutation detection, genetic disease detection, individual gene Type detection and other aspects. In many application fields, such as the diagnosis of infectious diseases, the sensitivity and specificity of nucleic acid detection technology are much better than colloidal gold / immunofluorescence method, and it is the gold standard for various diagnoses. However, almost all current nucleic acid detection technologies rely on instruments, and have high requirements for sample processing, detection environment, and personnel quality. A routine nucleic ac...

AI Technical Summary

Problems solved by technology

However, the reaction time of this method is too long (more than 4 hours), and it is difficult to meet the needs of rapid on-site detection.

(3) Recombinant polymerase amplification technology (RPA): The core protein of this technology is single-stranded DNA binding protein, single-stranded nucleic acid recombinase and strand-displacing DNA polymerase. This technology has strong amplification ability and fast speed (10-30 minutes ), with a wide temperature range (25-42°C), it is a potential isothermal amplification technique, but it also encounters the problem of false positives

However, there are still some deficiencies in other aspects. It also needs to go through multiple steps such as sample extraction, amplification, cutting, and color development. It faces problems such as uneven operator skills and contamination risks, and is not suitable for on-site rapid detection.

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