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Preparation and application of sandwich type lung cancer tumor marker immune sensor

A tumor marker and immunosensor technology, applied in the field of new nano functional materials and biosensors, can solve the problems of radioactive contamination and easy inactivation of enzymes, and achieve the effects of highly selective detection, convenient operation and simple preparation

Inactive Publication Date: 2014-11-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many clinical detection methods for lung cancer tumor markers, such as enzyme immunoassay, radioimmunoassay, etc., but these detection methods have many shortcomings, such as easy inactivation of enzymes in enzyme immunoassay, serious radioactive pollution in radioimmunoassay, etc.

Method used

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  • Preparation and application of sandwich type lung cancer tumor marker immune sensor
  • Preparation and application of sandwich type lung cancer tumor marker immune sensor
  • Preparation and application of sandwich type lung cancer tumor marker immune sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Lung cancer tumor marker secondary antibody (AuAgNRs-Ab) incubated with gold-silver core-shell nanorod sol 2 ) preparation.

[0030] Mix 20 mL of gold nanorod sol (AuNRs) and 20 mL of 0.05 mol / L cetyltrimethylammonium bromide (CTAB) solution, stir at 30 °C, and sequentially add 3 mL of 0.1 mol / L L- Ascorbic acid (AA) solution, 0.5 mL of 0.05 mol / L silver nitrate (AgNO 3 ) solution and 3 mL of 0.1 mol / L sodium hydroxide (NaOH) solution, stop stirring, let it stand for 1 hour, centrifuge, add 20 mL of PBS buffer solution with pH7.4, and then add lung cancer tumor marker secondary antibody (Ab 2 ), shaking at 4°C for 24h to obtain AuAgNRs-Ab 2 .

[0031] The AuNRs described are 20 nm long rod-shaped gold nanoparticles in aqueous solution.

Embodiment 2

[0032] Example 2 AuAgNRs-Ab 2 preparation.

[0033] Mix 20 mL of AuNRs and 20 mL of 0.1 mol / L CTAB solution, stir at 35 °C, and sequentially add 4 mL of 0.1 mol / L AA solution, 0.8 mL of 0.05 mol / L AgNO 3 solution and 4 mL of 0.1 mol / L NaOH solution, stop stirring, let it stand for 2 hours, centrifuge, add 20 mL of PBS buffer solution with pH7.4, and then add Ab 2 , shaking at 4°C for 24h to obtain AuAgNRs-Ab 2 .

[0034] The AuNRs described are 30 nm long rod-shaped gold nanoparticles in aqueous solution.

Embodiment 3

[0035] Example 3 AuAgNRs-Ab2 preparation.

[0036] Mix 20 mL of AuNRs and 20 mL of 0.2 mol / L CTAB solution, stir at 45 °C, and sequentially add 6 mL of 0.1 mol / L AA solution, 1 mL of 0.05 mol / L AgNO 3 solution and 6 mL of 0.1 mol / L NaOH solution, stop stirring, let it stand for 3 hours, centrifuge, add 20 mL of PBS buffer solution with pH7.4, and then add Ab 2 , shaking at 4°C for 24h to obtain AuAgNRs-Ab 2 .

[0037] The AuNRs described are 40 nm long rod-shaped gold nanoparticles in aqueous solution.

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Abstract

The invention discloses a preparation method of a sandwich type lung cancer tumor marker immune sensor and the application of the sandwich type lung cancer tumor marker immune sensor to lung cancer tumor marker detection, belonging to the fields of novel nanometer functional materials and a biosensor. According to the preparation method, a precious metal nanometer material, namely a gold @ silver core-shell nanorod is taken as biosimulation enzyme, a second antibody is taken as a marker after being incubated, and nanometer materials including a gold nanorod and reducing graphene are taken as electrode modifiers, so that the sandwich type electrochemical immune sensor which is used for detecting the lung cancer tumor marker and is low in cost, high in sensitivity, good in specificity, rapid in detection and simple in preparation can be prepared.

Description

technical field [0001] The invention relates to the preparation and application of a sandwich-type lung cancer tumor marker immunosensor. Specifically, a new nano functional material is used as a mimetic enzyme-labeled secondary antibody to realize rapid and sensitive detection of lung cancer tumor markers, which belongs to the technical field of new nano functional materials and biosensors. Background technique [0002] Lung cancer is one of the most threatening malignant tumors to the health and life of the population, and its morbidity and mortality are increasing the fastest. According to reports, the incidence of lung cancer has increased significantly in the past fifty years. Among male malignant tumor patients, the incidence and mortality of lung cancer accounted for the first place, and among female malignant tumor patients, the incidence and mortality both occupied the second place. . Therefore, early diagnosis and early treatment of lung cancer are the key to imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/26
CPCG01N33/5438G01N33/57423G01N33/57488G01N33/587
Inventor 张勇魏琴吴丹马洪敏庞雪辉
Owner UNIV OF JINAN
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