PCR detector

Abstract

The invention relates to a PCR detector. The PCR detector comprises a rotating device, a micro-fluidic chip and a temperature control device. The micro-fluidic chip comprises a chip main body, an extraction unit, a quantification unit and an amplification unit. The chip main body is arranged on the rotating device, and the chip main body is provided with a proximal end and a telecentric end. The extraction unit, the quantification unit and the amplification unit are sequentially communicated and are respectively arranged on the chip main body, and the extraction unit and the amplification unitare respectively arranged at the telecentric end of the chip main body when the chip main body rotates. The temperature control device comprises a heating assembly and a cooling assembly, the heatingassembly is used for heating the extraction unit and the amplification unit, the cooling assembly is used for cooling the extraction unit and the amplification unit, and the heating assembly is attached to the telecentric end when the chip body rotates. The PCR detector sequentially completes the two steps of nucleic acid extraction and amplification through the micro-fluidic chip, so that the operation link is reduced, and the nucleic acid detection time is further shortened. The heating assembly in the temperature control device has the advantages of high heating efficiency and high heat conduction efficiency.

Description

technical field [0001] The invention relates to the technical field of nucleic acid amplification, in particular to a PCR detector. Background technique [0002] Polymerase Chain Reaction (PCR) is a molecular biology reaction of DNA amplification. During the variable temperature PCR reaction, the extraction and amplification of nucleic acid and the temperature cycle conditions required by the reaction entity are the key steps to determine the accuracy of nucleic acid detection results. In the traditional variable temperature PCR reaction process, the operator performs the nucleic acid extraction step, and transfers the extracted nucleic acid reagent to the amplification test tube, and then uses a thermal cycle system to cycle the temperature of the amplification test tube, including high temperature denaturation, low temperature annealing, Mesophilic primer extension. The thermal cycle system of traditional PCR is roughly divided into water bath type, air flow type and sem...

AI Technical Summary

Problems solved by technology

[0003] Based on this, it is necessary to provide a PCR detector with high integration and high thermal cycle efficiency for the problems of cumbersome process, easy pollution and low thermal cycle efficiency in the traditional PCR reaction process.

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