Micro-fluidic chip, nucleic acid detection instrument and nucleic acid detection method

Abstract

The invention relates to a micro-fluidic chip, a nucleic acid detection instrument and a nucleic acid detection method, wherein the micro-fluidic chip comprises a chip main body, an extracting unit, aquantitating unit and an amplification unit, wherein the chip main body is provided with a distal end and a proximal end; the chip main body can rotate around a rotating center near the proximal endthrough being driven by a rotating device; the extracting unit, the quantitating unit and the amplification unit are respectively arranged at the chip main body; the quantitating unit communicates with the extracting unit; the amplification unit is positioned between the quantitating unit and the distal end; and the amplification unit communicates with the quantitating unit. The micro-fluidic chip, the nucleic acid detection instrument and the nucleic acid detection method have the advantages that the operation links of a nucleic acid reagent are reduced; the nucleic acid detection time is shortened; and the effective proceeding of the nucleic acid detection process is ensured.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a microfluidic chip, a nucleic acid detector and a nucleic acid detection method. Background technique [0002] Nucleic acid extraction and PCR amplification are important steps in the nucleic acid detection process, which have an important impact on the accuracy of nucleic acid detection results. At present, in the process of nucleic acid extraction and PCR amplification, it is generally used to process the specimen, extract the nucleic acid, and then transfer the nucleic acid product to the amplification tube. Due to the exposure of DNA or RNA to the air and the exposure of the amplification reagents to the air, the current operation steps have higher requirements on the environment, and it is necessary to build a professional nucleic acid testing laboratory. At the same time, pollution or contaminated Factors to limit, otherwise the nucleic acid detection process is pron...

AI Technical Summary

Problems solved by technology

[0003] Based on this, it is necessary to provide a microfluidic chip, a nucleic acid detector, and a nucleic acid detection method with a high degree of integration and effectively ensure the smooth progress of the nucleic acid detection process in view of the current nucleic acid detection process that has high environmental requirements and is prone to failure.

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