A method for rapid determination of rice gluten content based on quantum dots

A technology for rapid determination of gluten, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of poor selectivity, high detection cost, low sensitivity, etc., and achieve the effect of simple operation, low detection cost and high sensitivity

Inactive Publication Date: 2016-07-06
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

[0003] The existing domestic rice protein detection method is mainly a Kjeldahl Method (Kjeldahl Method), and a small amount of foreign countries use Coomassie Brilliant Blue G2250 to combine with rice gluten, and the product causes the maximum absorption peak of the dye. The reaction rate is about 2min, and the product is stable It is also better (about 1h), but there are problems such as low sensitivity, poor selectivity and high detection cost, and there are still great limitations in large-scale industrial production applications

Method used

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  • A method for rapid determination of rice gluten content based on quantum dots
  • A method for rapid determination of rice gluten content based on quantum dots
  • A method for rapid determination of rice gluten content based on quantum dots

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Embodiment 1

[0034] This embodiment includes the following steps:

[0035] (1) Preparation of CdTe quantum dots:

[0036] Weigh 25mgTe powder, 40mgNaBH 4 Put it into a small glass bottle, inject 1mL of distilled water into the small glass bottle with a syringe, keep the needle of the syringe on the cap of the glass bottle, and the needle cannot be covered by the solution in the glass bottle, react at a low temperature of 4°C for 4 hours in the dark, and obtain the reaction precursor substances;

[0037] Weigh 200mgCdCl 2 and 280mg of 2-mercaptoethylamine were dissolved in 160mL of distilled water, then the pH of the mixed solution was adjusted to 5.5 with 1mol / L hydrochloric acid, and then the mixed solution was transferred into a three-necked flask;

[0038] Into the three-necked flask N 2 , stir the mixed solution by magnetic force, wait until the temperature of the mixed solution rises to 90°C, condense and reflux, react for 40 minutes, inject the reaction precursor substance, react...

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Abstract

A method for rapidly determining rice glutenin content based on quantum dots, comprising the following steps: (1) preparation of CdTe quantum dots; (2) preparation of mother liquor for detection reaction; (3) rapid determination of rice glutenin content by quantum dots The establishment of the method; (4) The pretreatment when detecting rice gluten samples. The price of raw materials for preparing CdTe quantum dots in the present invention is relatively low, the preparation of quantum dots is simple, and the consumption amount is small when the quantum dots are used to quickly measure the glutenin content of rice, and the detection cost is low.

Description

technical field [0001] The invention relates to a method for measuring glutenin content in rice, in particular to a method for quickly measuring glutenin content in rice based on quantum dots. Background technique [0002] Rice protein is recognized as a high-quality edible protein. Initially, Osborne (1924) divided rice protein into albumin, globulin, gliadin and glutenin according to the solubility properties. Among them, glutenin and gliadin are also called storage proteins. Gluten accounts for more than 80% of the total protein (Osborne TB. The Vegetable Proteins, 2nded [M]. New York: Longmans, Green & Co, 1924, 154.). From nutritional analysis, only gluten and globulin can be digested and absorbed by the human body (KumamaruT, SatohH, LwataN, et al. Mutants for rice storage proteins [J]. THEORAPPLGENET, 1988, 76: 11-16.), and, in gluten Among them, the first limiting amino acid in rice—lysine (Lys) has the highest content, so glutenin not only has high nutritional valu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 孙术国林亲录杨涛徐友志丁玉琴罗非君周慧翁光辉
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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