A rapid on-site detection kit for nucleic acid without instrumentation

Abstract

The invention discloses an instrument-free on-site rapid detection kit for nucleic acid. The detection card includes a sample inlet, a sample processing module, a puncture tube, a reaction liquid chamber, a reaction liquid flow path, a main reaction area, n second reaction areas and corresponding n driving components, n≥0, temperature control area, Result areas, result-driven components, waste areas, enclosures, etc. The test card does not require pre-extraction and purification of nucleic acid samples, and realizes on-site detection from the original sample to the result. It is easy to operate, fast, and less prone to errors. It only requires two-step operations by non-professionals, which greatly reduces errors caused by different operators. It also avoids the risk of aerosol pollution. It has low requirements on sample processing, testing environment and personnel quality. The risk of pollution is small and the false positive rate is extremely low. It does not rely on large or expensive instruments and has low cost. It can be used directly It is suitable for nucleic acid detection of various types of complex and diverse samples on site, and is also suitable for a variety of similar steps and complex detection reactions. It has a wide range of applications and a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of detection. More specifically, it relates to an instrument-free nucleic acid on-site rapid detection kit. Background technique [0002] Nucleic acid detection technology, after decades of development and rapid maturity in recent years, has been widely used in infectious disease pathogen detection (including bacteria, mycoplasma, viruses, etc.), tumor mutation detection, genetic disease detection, individual gene detection Type testing, etc. In many application fields, such as infectious disease diagnosis, the sensitivity and specificity of nucleic acid detection technology are much better than colloidal gold / immunofluorescence method, and it is the gold standard for various diagnosis. However, almost all of the current nucleic acid detection technologies need to rely on instruments, and have high requirements on sample processing, testing environment and personnel quality. A routine nucleic acid detecti...

AI Technical Summary

Problems solved by technology

However, the reaction time of this method is too long (more than 4 hours), and it is difficult to meet the needs of rapid on-site detection.

(3) Recombinant polymerase amplification technology (RPA): The core protein of this technology is single-stranded DNA binding protein, single-stranded nucleic acid recombinase and strand-displacing DNA polymerase. This technology has strong amplification ability and fast speed (10-30 minutes ), with a wide temperature range (25-42°C), it is a potential isothermal amplification technique, but it also encounters the problem of false positives

However, there are still some deficiencies in other aspects. It also needs to go through multiple steps such as sample extraction, amplification, cutting, and color development. It faces problems such as uneven operator skills and contamination risks, and is not suitable for on-site rapid detection.

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