Nucleic-acid aptamer type photoelectric sensor for detecting atrazine and preparation method of such nucleic-acid aptamer type photoelectric sensor

Abstract

The invention relates to a nucleic-acid aptamer type photoelectric sensor for detecting atrazine and a preparation method of such nucleic-acid aptamer type photoelectric sensor. The preparation method includes firstly, preparing a three-dimensionally ordered macroporous 3DOM TiO2 / FTO electrode; then, performing Au nanoparticle modification through a hydrothermal reduction method so as to obtain an Au NPs / 3DOM TiO2 / FTO composite electrode; finally, combining an atrazine aptamer onto the surface of the Au NPs / 3DOM TiO2 / FTO composite electrode through an Au-S bond self-assembled film method so as to obtain an Aptamer / Au NPs / 3dom TiO2 / FTO sensor electrode. Compared with the prior art, the nucleic-acid aptamer type photoelectric sensor for detecting the atrazine and the preparation method have the advantages that the nucleic-acid aptamer is used as an atrazine recognition element, so that sensor detection selectivity is improved greatly; since a three-dimensionally ordered macroporous Au NPs / 3DOM TiO2 / FTO composite material of a microstructure is used for recognizing the molecular load of nucleic-acid aptamer element, the photoelectric catalysis performance can be enhanced effectively, atrazine detection sensitivity is improved, the limit of detection is as low as ng / L, high selective recognition is achieved, and the nucleic-acid aptamer type photoelectric sensor can be applied to detection and analysis of trace pollutants accordingly.

Description

technical field

[0001] The invention belongs to the technical fields of nanomaterials, photoelectrochemical analysis and environmental detection, and relates to a nucleic acid aptamer photosensor for detecting atrazine and a preparation method thereof. Background technique

[0002] Triazine herbicides (Triazine herbicides) are a traditional high-efficiency herbicide and the most common type of pesticide endocrine disruptors. They have been used as early as the 1950s. This type of herbicide is used in large amounts, has a long residual time, and has good water solubility. When used, it is very easy to cause pollution to soil, crops, and surface water. Discharge into the sea will also cause serious harm to the marine environment and ecology. Among them, atrazine is considered to be one of the most polluting triazine herbicide pesticides. Studies have proved that atrazine has a great impact on the reproductive function of animals, and it has been recognized by the World Wildlif...

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