Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nucleic-acid aptamer type photoelectric sensor for detecting atrazine and preparation method of such nucleic-acid aptamer type photoelectric sensor

A technology of nucleic acid aptamer and photoelectric sensor, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of limiting the organic combination of biologically active substances and TiO2, low oxidation efficiency of titanium dioxide, and limited utilization of light, etc., to achieve photoelectricity Strong catalytic activity, improved photoelectrochemical performance, and good reproducibility

Inactive Publication Date: 2017-08-18
TONGJI UNIV
View PDF8 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] TiO 2 It is a common n-type semiconductor material, which has the advantages of high catalytic efficiency, strong oxidation ability, stable photochemical properties, non-toxic and harmless, cheap price, and no secondary pollution. Limited to the use of light, that is, it can only absorb energy in the ultraviolet region; on the other hand, the recombination of electron and hole pairs generated by photoexcitation also makes the oxidation efficiency of titanium dioxide lower
In addition, although the structure of the nucleic acid aptamer itself is relatively stable, it will lose its biological activity under long-term ultraviolet light irradiation, which greatly limits the ability of biologically active substances and TiO 2 organic combination of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic-acid aptamer type photoelectric sensor for detecting atrazine and preparation method of such nucleic-acid aptamer type photoelectric sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of the nucleic acid aptamer photosensor used to detect atrazine in this embodiment is as follows:

[0044] (1) Pretreatment of FTO electrodes. Before the 1.5cm×4.0cm FTO is used to prepare electrodes, it needs to undergo strict surface treatment to ensure that the surface is clean. Put the determined FTO into surfactant solution, distilled water, acetone, and absolute ethanol in order to ultrasonically clean for 15 minutes each, and then put it into deionized water to ultrasonically clean twice, each for 15 minutes. Afterwards, the FTO was taken out and dried in an air atmosphere.

[0045] (2) Self-assembled PS microsphere arrays on the surface of FTO electrodes. Put the pretreated FTO electrode vertically into 0.1wt% PS suspension, place it in an oven and dry it at 40°C, and a uniform and ordered array of PS microspheres is obtained on the FTO surface.

[0046] (3) Preparation of 3DOM TiO by sol-gel method 2 / FTO electrode. Configure TiO fir...

Embodiment 2

[0052] Photoelectrochemical detection of atrazine using the assembled nucleic acid aptamer photoelectrochemical biosensor.

[0053] Using the traditional three-electrode system to prepare Aptamer / Au NPs / 3DOM TiO 2 / FTO is the working electrode, the saturated calomel electrode is the reference electrode, the platinum wire electrode is the counter electrode, and the 0.1M PBS buffer solution containing 30mL 0.1M AA is the supporting electrolyte. A series of atrazine standard solutions with different concentrations were gradually added to the system by the standard addition method at room temperature for 30 minutes, and a bias voltage of 0.5V was applied. The current is converted into photocurrent density (j) according to the working area of ​​the electrode. Through the analysis results, it was found that within a certain concentration range of atrazine, with the increase of the concentration of atrazine, the photocurrent density also increased correspondingly. On the electrode ...

Embodiment 3

[0055] The assembled nucleic acid aptamer photoelectrochemical biosensor was used for selection performance detection.

[0056] Aptamer / Au NPs / 3DOM TiO prepared by 2 / FTO is the working electrode, the saturated calomel electrode is the reference electrode, the platinum wire electrode is the counter electrode, and the 0.1M PBS buffer solution containing 30mL 0.1M AA is the supporting electrolyte. Apply a bias voltage of 0.5V, and use the I-t curve method to measure the photocurrent of the mixed system containing 0.01 μg / L atrazine solution and 100 times molar concentration of atrazine interfering substances under visible light excitation. Adding 0.01 μg / L atrazine will cause a rapid and obvious increase in photocurrent, but adding 1.0 μg / L trichlorfon, humic acid, PCBs, paraquat, acetamiprid, bisphenol A It did not cause obvious photocurrent changes; furthermore, adding 0.01 μg / L atrazine into the electrolyte system finally caused obvious photocurrent changes, and the interfer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a nucleic-acid aptamer type photoelectric sensor for detecting atrazine and a preparation method of such nucleic-acid aptamer type photoelectric sensor. The preparation method includes firstly, preparing a three-dimensionally ordered macroporous 3DOM TiO2 / FTO electrode; then, performing Au nanoparticle modification through a hydrothermal reduction method so as to obtain an Au NPs / 3DOM TiO2 / FTO composite electrode; finally, combining an atrazine aptamer onto the surface of the Au NPs / 3DOM TiO2 / FTO composite electrode through an Au-S bond self-assembled film method so as to obtain an Aptamer / Au NPs / 3dom TiO2 / FTO sensor electrode. Compared with the prior art, the nucleic-acid aptamer type photoelectric sensor for detecting the atrazine and the preparation method have the advantages that the nucleic-acid aptamer is used as an atrazine recognition element, so that sensor detection selectivity is improved greatly; since a three-dimensionally ordered macroporous Au NPs / 3DOM TiO2 / FTO composite material of a microstructure is used for recognizing the molecular load of nucleic-acid aptamer element, the photoelectric catalysis performance can be enhanced effectively, atrazine detection sensitivity is improved, the limit of detection is as low as ng / L, high selective recognition is achieved, and the nucleic-acid aptamer type photoelectric sensor can be applied to detection and analysis of trace pollutants accordingly.

Description

technical field [0001] The invention belongs to the technical fields of nanomaterials, photoelectrochemical analysis and environmental detection, and relates to a nucleic acid aptamer photosensor for detecting atrazine and a preparation method thereof. Background technique [0002] Triazine herbicides (Triazine herbicides) are a traditional high-efficiency herbicide and the most common type of pesticide endocrine disruptors. They have been used as early as the 1950s. This type of herbicide is used in large amounts, has a long residual time, and has good water solubility. When used, it is very easy to cause pollution to soil, crops, and surface water. Discharge into the sea will also cause serious harm to the marine environment and ecology. Among them, atrazine is considered to be one of the most polluting triazine herbicide pesticides. Studies have proved that atrazine has a great impact on the reproductive function of animals, and it has been recognized by the World Wildlif...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3275
Inventor 刘梅川丁雪赵国华
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com