Preparation method of electrochemical nonyl phenol sensor on basis of doped titanium dioxide/molybdenum disulfide composite material

A technology of molybdenum disulfide and composite materials, which is applied in the field of preparation of electrochemical biosensors, can solve the problems of low yield and cumbersome process, and achieve the effects of low cost, simple preparation, and increased electron transfer capacity

Inactive Publication Date: 2016-11-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Cu-TiO 2 / MoS 2 preparation of

[0038] (1) Add 0.6 g of molybdenum disulfide powder and 0.2 mmol of copper salt to 3 mL of n-butyllithium solution, and stir for 12 hours under nitrogen protection at 60 °C to obtain the reacted solution;

[0039] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 60 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain copper Intercalated molybdenum disulfide nanomaterials;

[0040] (3) Take 500 mg of copper-intercalated molybdenum disulfide nanomaterials prepared in step (2) and add them to 5 mL of tetrabutyl titanate. After stirring for 1 hour, slowly add 0.5 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 160°C for 18 hours;

[0041] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and the...

Embodiment 2

[0046] Example 2 Cu-TiO 2 / MoS 2 preparation of

[0047] (1) Add 0.6 g of molybdenum disulfide powder and 1.0 mmol of copper salt to 5 mL of n-butyllithium solution, and stir for 24 hours under nitrogen protection at 30 °C to obtain the reacted solution;

[0048] (2) Use a non-polar solvent to wash the solution after the reaction in step (1), and then perform ultrasonic treatment in a water bath at 30 °C. After the treatment, use a non-polar solvent to wash the treated solution, and dry it in vacuum to obtain copper Intercalated molybdenum disulfide nanomaterials;

[0049] (3) Take 200 mg of copper-intercalated molybdenum disulfide nanomaterials prepared in step (2) and add them to 5 mL of tetrabutyl titanate. After stirring for 1 hour, slowly add 0.6 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 180°C for 20 hours;

[0050] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute e...

Embodiment 3

[0055] Example 3 Cu-TiO 2 / MoS 2 preparation of

[0056] (1) Add 0.6 g of molybdenum disulfide powder and 2.0 mmol of copper salt to 10 mL of n-butyllithium solution, and stir for 48 hours under nitrogen protection at 50 °C to obtain the reacted solution;

[0057] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 50 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain copper Intercalated molybdenum disulfide nanomaterials;

[0058] (3) Take 10 mg of copper-intercalated molybdenum disulfide nanomaterials prepared in step (2) and add them to 5 mL of tetrabutyl titanate. After stirring for 1 hour, slowly add 0.8 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 200°C for 24 hours;

[0059] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and the...

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Abstract

The invention discloses a preparation method of an electrochemical biosensor. According to the method, a novel two-dimensional manometer electrode material-doped titanium dioxide / molybdenum disulfide composite material Cu-TiO2 / MoS2 is firstly prepared, and the material is a copper-doped titanium dioxide manometer square in-situ composite molybdenum disulfide two-dimensional manometer composite material; good biocompatibility and large specific surface areas of the material are utilized; a nonyl phenol antibody is loaded; during detection, copper-doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ; an electrochemical signal is generated; the influence of the specificity quantitative combination of the antibody and an antigen on the electronic transmission capability is utilized, so that the current intensity is correspondingly reduced; the building of a biosensor for detecting nonyl phenol by an unmarked electrochemical method is finally realized.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Nonylphenol is a bisphenol-like synthetic estrogen. At present, the methods for detecting nonylphenol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional training before they can perform...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48G01N27/26G01N21/76
CPCG01N21/76G01N27/26G01N27/3275G01N27/48
Inventor 张勇杜斌马洪敏吴丹范大伟
Owner UNIV OF JINAN
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