90results about How to "Rapid selective detection" patented technology

The invention relates to one-dimensional organic semiconductor nanowire/nanoribbon with ultrasensitive (ppb grade) fluorescence response to organophosphorus ester and related organic phosphorus-containing compound toxic gases and a preparation method and application thereof. The nanowire or nanoribbon is obtained by self-assembling of various perylene-containing amphiphilic perylene bisimide derivatives through Pi-Pi interaction. The perylene-containing amphiphilic perylene bisimide derivative has asymmetric amphiphilic substituent, wherein one end is a nonpolar straight-chain or branched-chain long-chain alkyl, and the other end is aryl containing polar group or aryl alkyl. According to a film formed by weaving the one-dimensional organic semiconductor nanowires or nanoribbons, detection concentration of steam of toxic organic phosphorus-containing compounds reaches the grade of several ppb to one hundred and more ppm. The product has a great application prospect.

The invention discloses a photoelectrochemical sensor for estradiol based on a boron-doped iron cobalt oxide two-dimensional nano composite material as well as a preparation method and application of the photoelectrochemical sensor. The preparation method comprises the following steps: firstly, modifying a two-dimensional nano material g-C3N4 on ITO (Indium Tin Oxide) conductive glass by adopting an electrodeposition method; secondly, carrying out in-situ grown of iron cobalt oxide by adopting a hydrothermal method, and further preparing to obtain a working electrode loaded with the g-C3N4, doped with boron and modified by the iron cobalt oxide Bi@Fe*Co<1->*O3/g-C3N4; finally, loading an estradiol antibody by using good biocompatibility and large specific surface area of the material. During detection, L-ascorbic acid-2-trisodium phosphate (AAP) can be catalyzed by boron-doped iron cobalt oxide Bi@Fe*Co<1->*O3 to generate L-ascorbic acid AA in situ, further an electron donor is provided for photoelectric detection, and then photocurrent intensity is correspondingly reduced by using the influence of specific combination of the antibody and antigen on electronic transmission capacity; finally, the construction of the photoelectrochemical sensor for the estradiol by adopting a mark-free photoelectrochemical detection method is realized.

The invention discloses a preparing method of an electrochemistry parathion sensor based on a metal and metal oxide co-doped nanometer composite, and belongs to the technical field of novel nanometer functional materials and biosensors. The preparing method comprises the steps that firstly, a novel two-dimensional manometer material Co-MoO3/TiO2@g-C3N4 is prepared, and a parathion antibody is loaded by using the good biocompatibility and the large specific area of the material; secondly, horse radish peroxidase is fixed through the cross-linking action of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate electrochemistry signals, then the influence on the electron transmittability by the specific and quantitative combination of the antibody and an antigen is used, the current intensity is reduced correspondingly, and finally the parathion detecting electrochemistry biosensor low in cost, high in sensitivity and specificity, fast in detection and easy to prepare is obtained.

The invention discloses a preparation method of an electrogenerated chemiluminescence sensor, and belongs to the technical field of novel nano-functional materials and biosensors. A novel two-dimensional nano-composite material is firstly prepared, manganese doped titanium dioxide nano-blocks are compounded with molybdenum disulfide in situ to form the two-dimensional nano-composite material Mn-TiO2/MoS2, aflatoxin antibodies are loaded by the aid of excellent biocompatibility and large specific surface area of the material, manganese doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ when the aflatoxin is detected, electrochemical reaction of O2 and K2S2O8 in base solution are performed to generate electrogenerated chemiluminescence signals, electronic transmission capacity is affected by specific quantitative combination of the antibodies and antigen, so that current intensity is reduced, luminescent intensity is reduced, and the electrogenerated chemiluminescence sensor for detecting the aflatoxin by an unmarked electrogenerated chemiluminescence method is finally prepared.

The invention relates to a manufacturing method for a hydrogen peroxide non-enzyme sensor based on a silver-graphene nano composite. The manufacturing method comprises the following specific steps of: 1) weighing 5-10mg of graphene oxide, and dissolving the graphene oxide into 5-10 mL of water, thereby obtaining a brown yellow graphene oxide solution; 2) taking a mixed solution containing AgNO3 and KNO3 as an electro-deposition solution; 3) transferring and dispensing 10-15muL of the graphene oxide solution obtained in the step 1) to the surface of a pre-treated glass carbon electrode, and drying in air at the room temperature, thereby obtaining a graphene oxide modified glass carbon electrode; and 4) taking the graphene oxide modified glass carbon electrode as a working electrode, taking a platinum wire electrode as a counter electrode, taking a statured mercurous chloride electrode as a reference electrode, putting the electrodes into the electro-deposition solution prepared in step 2), depositing for 1-3 minutes under constant potential within a range of -0.55V to -0.75V, thereby obtaining a silver-graphene nano composite modified electrode which is used as the hydrogen peroxide non-enzyme sensor. The sensor realizes quick, sensitive and high-selection detection on hydrogen peroxide, and has remarkable advantages in comparison with sensors reported in existing literature.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

The invention discloses a preparation method of electrochemical sensor for sulfonamide molecule, and belongs to the technical field of new nano functional materials and biosensor. The method firstly prepares a nickel hydroxide nanosheet array on a disposable throwable electrode, and uses the large specific surface area and a high activity hydroxyl functional group and an amino functional group ofpolydopamine to sequentially prepare a polydopamine film containing an electron mediator and a molecularly imprinted polymer taking a sulfonamide molecule as a template molecule directly on the nickelhydroxide nanosheet array by adopting an in-situ growth method, after eluting the template molecule, the position of the original template molecule becomes a hole, that is, the molecularly imprintedpolymer eluting the template molecule, thus, an electrochemical sensor for the sulfonamide molecule is prepared.

The invention discloses a preparation method of a macrolide antibiotic sensor based on iron nitride, and belongs to the technical field of new nano functional materials and chemical biosensors. The method comprises the following steps: firstly preparing an array of iron nitride nanosheets on a disposable electrode, directly preparing a polydopamine film and a molecularly imprinted polymer using the macrolide antibiotic molecule as a template molecule coated with luminol in situ successively on the array of iron nitride nanosheets one after another by adopting an in-situ growth method and utilizing a large specific surface area and a high adsorption activity for amidogen, and eluting the molecularly imprinted polymer of the template molecule after the template molecule is eluted and the original position of the template molecule became a hole, thereby preparing a macrolide antibiotic sensor based on iron nitride.

The invention discloses a preparation method for a tetracycline antibiotic electrochemiluminescence sensor, and belongs to the technical field of novel nanometer function materials and biosensing analysis. The preparation method firstly prepares a nickel-iron bimetallic oxide nanosheet array on a disposable electrode, the large specific surface area of the nickel-iron bimetallic oxide nanosheet array, high adsorption activity for an amino and the amino functional group of polydopamine are used, an in-situ growth method is adopted to successively directly preparing a polydopamine film and a molecularly imprinted polymer which coats luminol in an in-situ way and takes tetracycline antibiotics as a template molecule on the nickel-iron bimetallic oxide nanosheet array, and after the template molecule is eluted, the position of the original template molecule becomes a cavity, i.e., the molecularly imprinted polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic electrochemiluminescence sensor finishes being prepared.

The invention discloses a preparation method of a nitrofuran antibiotic sensor, and belongs to the technical field of new nano functional materials and biosensing analysis technology. The method comprises the following steps: firstly preparing an array of cobalt-nickel bimetal layered hydroxide nanosheets on a disposable electrode, directly preparing a polydopamine film containing an electron mediator and a molecularly imprinted polymer using the nitrofuran antibiotic as a template molecule successively on the array of cobalt-nickel bimetal layered hydroxide nanosheets one after another by adopting an in-situ growth method and utilizing a large specific surface area and high active hydroxyl functional groups, and eluting the molecularly imprinted polymer of the template molecule after thetemplate molecule is eluted and the original position of the template molecule became a hole, thereby preparing a nitrofuran antibiotic sensor.

The invention discloses a preparing method of an electrochemiluminescence carbamate sensor based on a cobalt-doped two-dimensional nanometer composite, and belongs to the technical field of novel nanometer functional materials and biosensors. The preparing method comprises the steps that firstly, a novel two-dimensional manometer material Co-MoO3/TiO2@g-C3N4 is prepared, and a carbamate antibody is loaded by using the good biocompatibility and the large specific area of the material; secondly, horse radish peroxidase is fixed through the cross-linking action of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ and is subjected to an electrochemical reaction with a base solution to generate high and stable electrochemiluminescence signals, then the influence on the electron transmittability by the specific and quantitative combination of the antibody and an antigen is used, the electrochemiluminescence signals are reduced correspondingly, and finally the carbamate detecting electrochemiluminescence biosensor low in cost, high in sensitivity and specificity, fast in detection and easy to prepare is obtained.

The invention discloses a preparation method of a photoelectrochemical furazolidone sensor, and belongs to the technical fields of novel nano functional materials and biosensors. According to the preparation method, a novel dual metal co-doped two-dimensional photosensitizer is prepared at first, namely, iron and manganese co-doped titanium dioxide nano blocks and molybdenum disulfide carry out in-situ synthesis to generate a two-dimensional nano composite material (FeMn-TiO2/MoS2), which has a good biocompatibility and large specific area, then a furazolidone antibody is loaded on the two-dimensional nano composite material, alkaline phosphatase is fixed on the two-dimensional nano composite material; when the photosensitizer is used for detection, alkaline phosphatase can catalyze L-ascorbic acid-2-phosphate trisodium salt (AAP) to generate L-ascorbic acid (AA) in-situ; thus electron donors are provided for photoelectric detection; due to the influence of specific and quantitative combination between antibody and antigen on the electron transmission performance, the photoelectric current strength is correspondingly reduced, and finally a photoelectric sensor for detecting furazolidone through a label-free photoelectric method is manufactured.

The invention discloses a manufacturing method and application of an electrochemiluminescence nonyl phenol sensor based on a titanium dioxide nanosheet composite and belongs to the technical field of novel nano functional materials and bio-sensors. The manufacturing method includes: preparing a novel two-dimensional sheet magnetic nano photosensitive material FeCo-N@TiO2; utilizing good biocompatibility, large specific surface area and ferromagnetism to load an upper nonyl phenol antibody; detecting through horse radish peroxidase having cross-linking effect, wherein the horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ; enabling the oxygen and a base solution to be in electrochemical reaction to generate high and stable electrochemiluminescence signals, utilizing influence on electron transport capability by specific quantitative combining of the antibody and antigen to correspondingly reduce the electrochemiluminescence signals, and finally obtaining the electrochemiluminescence bio-sensor which is low in cost, high in sensitivity and specificity, quick in detection, simple to manufacture and supportive of detection on nonyl phenol.

The invention discloses a fabrication method of a tetracycline antibiotic molecular imprinting electrochemical sensor, and belongs to the technical field of novel nanometer functional material and chemical biological sensor. A nickel-iron thermometal nitride nanosheet array is fabricated on a disposable throwable electrode, an electronic medium-containing polydopamine thin film and a molecular imprinting polymer taking ampicillin as a template molecule polymer are directly and sequentially fabricated on the nickel-iron thermometal layered hydroxide nanosheet array by an in-situ growth method according to large specific surface area, high-activity hydroxyl functional group and amino functional group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecule imprinting polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic molecular imprinting electrochemical sensor is fabricatedand completed.

The invention discloses a method for preparing electrochemical biphenol A sensors. The method has the advantages that a novel two-dimensional nanometer electrode material Mn-TiO2/MoS2, namely, a two-dimensional nanometer composite material, is prepared at first, manganese-doped titanium dioxide nanometer blocks and molybdenum disulfide are subjected to in-situ composition to obtain the two-dimensional nanometer composite material, the novel two-dimensional nanometer electrode material is excellent in biocompatibility, has a large specific surface area and is loaded with biphenol A antibodies, hydrogen peroxide can be catalyzed by manganese-doped titanium dioxide to generate O2 in an in-situ manner, electrochemical signals can be generated, the current intensity can be correspondingly lowered by the aid of specific quantification of the antibodies and antigens and influence on the electron transport capacity, and accordingly the biochemical biphenol A sensors which are biosensors for detecting biphenol A by the aid of label-free electrochemical processes can be ultimately constructed.

The invention discloses a preparation method of an electrochemical sensor constructed on the basis of a nano functional material and belongs to the technical field of novel nano functional materials and biosensors. The preparation method includes: preparing a novel two-dimensional nano electrode material-the loaded bi-metal co-doped nano composite material FeCo-TiO2/MoS2, namely a two-dimensional nano composite material with an iron-cobalt co-doped titanium dioxide nano square block composited with molybdenum disulfide in situ; utilizing high biocompatibility and large specific surface area of the material to load a clenbuterol antibody; during detection, due to the fact that iron-cobalt co-doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ to generate electrochemical signals, utilizing influence of specific quantitative combining of the antibody and antigen on electron transmission capability to correspondingly lower current strength; finally realizing construction of a biosensor adopting a mark-free electrochemical method to detect clenbuterol.

The invention discloses a preparation method of a photoelectrochemical chlordimeform sensor, belonging to the technical field of novel nanometer functional materials and biological sensors. Firstly, a novel load type bimetal codoped photosensitizer, namely a two-dimensional nanocomposite material FeCo-TiO2/MoS2 compounded from ferrous-cobalt codoped titanium dioxide nanodiamond with molybdenum disulfide in situ is prepared, and by utilizing the good biocompatibility and large specific surface area of the material, a chlordimeform antibody is loaded and alkaline phosphatase is immobilized; in detection, as the alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate AAP to generate L-ascorbic acid AA in situ and further provide electron donors for photoelectric detection, the photocurrent intensity is correspondingly reduced by utilizing the influence of specific quantitative binding of the antibody and antigen on the electron transmission capability, and finally the construction of the photoelectric sensor for detecting the chlordimeform by adopting an unmarked photoelectrochemical method can be realized.