Preparation method and application of electrochemiluminescent bisphenol A biosensor based on nitrogen-doped titanium dioxide nanosheet

A biosensor, titanium dioxide technology, applied in chemiluminescence/bioluminescence, analysis through chemical reaction of materials, scientific instruments, etc., can solve the problems of low sensitivity, poor conductivity, and unfavorable practical application of electrochemiluminescence sensors , to achieve the effect of increasing the intensity of electrochemiluminescence, improving the intensity and stability, and improving the ability of resonance energy transfer

Inactive Publication Date: 2016-06-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemiluminescence sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 FeMn-NTiO 2 preparation of

[0038] First, take 0.8mmol of iron salt, 0.8mmol of manganese salt and 1mmol of ammonium salt and add them to 5mL of tetrabutyl titanate. During stirring, slowly add 0.5mL of hydrofluoric acid, react in the reactor at 160°C for 24 hours, and cool After reaching room temperature, centrifuge and wash with ultrapure water and absolute ethanol three times, then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 1°C / min, and calcinate at 480°C for 60min ; Finally, the calcined powder is cooled to room temperature to obtain FeMn-NTiO 2 ;

[0039] Described iron salt is ferric sulfate;

[0040] Described manganese salt is manganese sulfate;

[0041] Described ammonium salt is ammonium sulfate.

Embodiment 2

[0042] Example 2 FeMn-NTiO 2 preparation of

[0043] First, add 0.8mmol of iron salt, 1.0mmol of manganese salt and 1mmol of ammonium salt into 5mL of tetrabutyl titanate. During stirring, slowly add 0.65mL of hydrofluoric acid, react in the reactor at 180°C for 21 hours, and cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 2°C / min, and calcinate at 520°C for 30min ; Finally, the calcined powder is cooled to room temperature to obtain FeMn-NTiO 2 ;

[0044] Described iron salt is ferric chloride;

[0045] Described manganese salt is manganese chloride;

[0046] Described ammonium salt is ammonium chloride.

Embodiment 3

[0047] Example 3 FeMn-NTiO 2 preparation of

[0048] First, take 0.8mmol of iron salt, 1.2mmol of manganese salt and 1mmol of ammonium salt and add them to 5mL of tetrabutyl titanate. During the stirring process, slowly add 0.8mL of hydrofluoric acid, react in the reactor at 200°C for 18 hours, and cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, and then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 3°C / min, and calcinate at 560°C for 10 min ; Finally, the calcined powder is cooled to room temperature to obtain FeMn-NTiO 2 ;

[0049] Described iron salt is ferric nitrate;

[0050] Described manganese salt is manganese nitrate;

[0051] Described ammonium salt is ammonium nitrate.

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Abstract

The invention discloses a preparation method of an electrochemiluminescent bisphenol A biosensor based on a nitrogen-doped titanium dioxide nanosheet and belongs to the technical field of novel nano functional materials and biosensors. A novel flaky magnetic nano light-sensitive material FeMn-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a bisphenol A antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ, and an electrochemical reaction is generated with a base solution, high and stable electrochemiluminescence signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the electrochemiluminescence signals are correspondingly reduced, and finally the electrochemiluminescent biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects bisphenol A is prepared.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescent bisphenol A biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Bisphenol A is a bisphenol-like synthetic estrogen. [0003] At present, the methods for detecting bisphenol A mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/5438
Inventor 张勇吴丹王耀光范大伟魏琴
Owner UNIV OF JINAN
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