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Preparation method and application of electrochemical malathion biosensor based on bi-metal in-situ composite two-dimensional nanomaterial

A two-dimensional nanomaterial, biosensor technology, applied in scientific instruments, analytical materials, material analysis by electromagnetic means, etc., can solve the problems of poor conductivity, unfavorable practical application, low sensitivity of electrochemical sensors, etc., to improve the strength and Effects of stability, increasing electron transfer ability, and improving resonance energy transfer ability

Inactive Publication Date: 2016-06-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemical sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 FeMn-NTiO 2 Preparation

[0036] First, take 0.8mmol iron salt, 0.8mmol manganese salt and 1mmol ammonium salt and add to 5mL tetrabutyl titanate. During stirring, slowly add 0.5mL hydrofluoric acid, and react in the reaction kettle at 160℃ for 24 hours, cooling After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, and then vacuum dry at 50°C; then, put the ground powder into a muffle furnace at a heating rate of 1°C / min, and calcine at 480°C for 60 minutes ; Finally, the calcined powder is cooled to room temperature to obtain FeMn-NTiO 2 ;

[0037] The iron salt is iron sulfate;

[0038] The manganese salt is manganese sulfate;

[0039] The ammonium salt is ammonium sulfate.

Embodiment 2

[0040] Example 2 FeMn-NTiO 2 Preparation

[0041] First, add 0.8mmol iron salt, 1.0mmol manganese salt and 1mmol ammonium salt to 5mL tetrabutyl titanate. During stirring, slowly add 0.65mL hydrofluoric acid, and react in the reactor at 180°C for 21 hours. Cool down After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, and then vacuum dry at 50°C; then, put the ground powder into a muffle furnace at a heating rate of 2°C / min, and calcinate at 520°C for 30 minutes ; Finally, the calcined powder is cooled to room temperature to obtain FeMn-NTiO 2 ;

[0042] The iron salt is ferric chloride;

[0043] The manganese salt is manganese chloride;

[0044] The ammonium salt is ammonium chloride.

Embodiment 3

[0045] Example 3 FeMn-NTiO 2 Preparation

[0046] First, take 0.8mmol of iron salt, 1.2mmol of manganese salt and 1mmol of ammonium salt and add to 5mL of tetrabutyl titanate. During stirring, slowly add 0.8mL of hydrofluoric acid, and react in the reactor at 200°C for 18 hours. Cool down After reaching room temperature, it was centrifuged and washed three times with ultrapure water and absolute ethanol, and then dried in vacuum at 50°C; then, the ground powder was put into a muffle furnace with a heating rate of 3°C / min and calcined at 560°C for 10 minutes ; Finally, the calcined powder is cooled to room temperature to obtain FeMn-NTiO 2 ;

[0047] The iron salt is iron nitrate;

[0048] The manganese salt is manganese nitrate;

[0049] The ammonium salt is ammonium nitrate.

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Abstract

The invention discloses a preparation method and application of an electrochemical malathion biosensor based on a bi-metal in-situ composite two-dimensional nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel flaky magnetic nano light-sensitive material FeMn-N@TiO2 is prepared for the first time; through good bio-compatibility, large specific area and ferromagnetism of the material, a malathion antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects malathion is prepared.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical malathion biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Malathion, also known as Malathion, is a drug insecticide suitable for controlling pests on crops such as tobacco, tea and mulberry. Malathion has good contact, stomach toxicity and certain fumigation effect, without systemic effect. In soil, malathion can slightly migrate to the deep soil layer through water leaching. Malathion in the soil can enter the plant through absorption by plant roots. After people eat such plants or plants containing their residues by mistake, malathion can enter the human body through the digestive tract, respiratory tract and intact skin and mucous membranes, causing nausea, vomiting, headache, diarrhea, general weakness and other poisoning Initial symptoms, long-term exposure can cause cancer. [0003] At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 张勇马洪敏任祥王耀光庞雪辉
Owner UNIV OF JINAN
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