Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of electrogenerated chemiluminescence sensor for detecting aflatoxin

A technology of aflatoxin and electrochemistry, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, scientific instruments, etc., can solve the problems of cumbersome process and low yield, and achieve simple preparation, low cost, large scale Effect of ECL Intensity

Inactive Publication Date: 2016-12-07
UNIV OF JINAN
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Mn-TiO 2 / MoS 2 preparation of

[0042] (1) Add 0.6 g of molybdenum disulfide powder and 0.2 mmol of manganese salt to 3 mL of n-butyllithium solution, and stir for 12 hours under nitrogen protection at 60 °C to obtain the reacted solution;

[0043] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 60 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain manganese Intercalated molybdenum disulfide nanomaterials;

[0044] (3) Take 500 mg of manganese-intercalated molybdenum disulfide nanomaterials prepared in step (2) and add them to 5 mL of tetrabutyl titanate. After stirring for 1 hour, slowly add 0.5 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 160°C for 18 hours;

[0045] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol...

Embodiment 2

[0050] Example 2 Mn-TiO 2 / MoS 2 preparation of

[0051] (1) Add 0.6 g of molybdenum disulfide powder and 1.0 mmol of manganese salt to 5 mL of n-butyllithium solution, and stir for 24 hours under nitrogen protection at 30 °C to obtain the reacted solution;

[0052] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 30 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain manganese Intercalated molybdenum disulfide nanomaterials;

[0053] (3) Add 200 mg of manganese-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, then slowly add 0.6 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 180°C for 20 hours;

[0054] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum...

Embodiment 3

[0059] Example 3 Mn-TiO 2 / MoS 2 preparation of

[0060] (1) Add 0.6 g of molybdenum disulfide powder and 2.0 mmol of manganese salt into 10 mL of n-butyllithium solution, and stir for 48 hours under nitrogen protection at 50 °C to obtain the reacted solution;

[0061] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 50 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain manganese Intercalated molybdenum disulfide nanomaterials;

[0062] (3) Add 10 mg of the manganese-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, slowly add 0.8 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 200°C for 24 hours;

[0063] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of an electrogenerated chemiluminescence sensor, and belongs to the technical field of novel nano-functional materials and biosensors. A novel two-dimensional nano-composite material is firstly prepared, manganese doped titanium dioxide nano-blocks are compounded with molybdenum disulfide in situ to form the two-dimensional nano-composite material Mn-TiO2 / MoS2, aflatoxin antibodies are loaded by the aid of excellent biocompatibility and large specific surface area of the material, manganese doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ when the aflatoxin is detected, electrochemical reaction of O2 and K2S2O8 in base solution are performed to generate electrogenerated chemiluminescence signals, electronic transmission capacity is affected by specific quantitative combination of the antibodies and antigen, so that current intensity is reduced, luminescent intensity is reduced, and the electrogenerated chemiluminescence sensor for detecting the aflatoxin by an unmarked electrogenerated chemiluminescence method is finally prepared.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescent aflatoxin sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Aflatoxins are a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. Aflatoxins exist in soil, animals, plants, and various nuts, especially grain and oil products such as peanuts, corn, rice, soybeans, and wheat. They are the most toxic mycotoxins and are extremely harmful to human health. . Aflatoxins mainly include aflatoxin M1, aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2. Aflatoxin is classified as a class 1 carcinogen by the World Health Organization, 68 times more toxic than arsenic, second only to botulinum toxin, and is the most toxic mold poisoning known so far. The harmfulness of aflatoxin lies in its destructive effect on the liver tissue of humans and animal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
CPCG01N21/76G01N27/305
Inventor 史御书郑娜张国庆张勇
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com