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Preparation method and application of electrochemical diethylstilbestrol sensor based on titanium-dioxide-based two-dimensional composite nanomaterial

A technology of diethylstilbestrol and titanium dioxide is applied in scientific instruments, analytical materials, material analysis by electromagnetic means, etc., which can solve the problems of poor conductivity, low sensitivity of electrochemical sensors, and unfavorable practical applications, and achieves improved strength and stability. The effect of improving resonance energy transfer ability and increasing electron transfer ability

Inactive Publication Date: 2016-06-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemical sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 FeCo-NTiO 2 preparation of

[0035] First, take 0.8mmol of iron salt, 0.8mmol of cobalt salt and 1mmol of ammonium salt and add them to 5mL of tetrabutyl titanate. During stirring, slowly add 0.5mL of hydrofluoric acid, react in the reactor at 160°C for 24 hours, and cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, and then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 1°C / min, and calcinate at 480°C for 60min ; Finally, the calcined powder is cooled to room temperature to obtain FeCo-NTiO 2 ;

[0036] Described iron salt is ferric sulfate;

[0037] Described cobalt salt is cobalt sulfate;

[0038] Described ammonium salt is ammonium sulfate.

Embodiment 2

[0039] Example 2FeCo-NTiO 2 preparation of

[0040] First, take 0.8mmol iron salt, 1.0mmol cobalt salt and 1mmol ammonium salt and add them to 5mL tetrabutyl titanate. During the stirring process, slowly add 0.65mL hydrofluoric acid, react in the reactor at 180°C for 21 hours, and cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 2°C / min, and calcinate at 520°C for 30min ; Finally, the calcined powder is cooled to room temperature to obtain FeCo-NTiO 2 ;

[0041] Described iron salt is ferric chloride;

[0042] Described cobalt salt is cobalt chloride;

[0043] Described ammonium salt is ammonium chloride.

Embodiment 3

[0044] Example 3 FeCo-NTiO 2 preparation of

[0045] First, add 0.8mmol iron salt, 1.2mmol cobalt salt and 1mmol ammonium salt to 5mL tetrabutyl titanate. During stirring, slowly add 0.8mL hydrofluoric acid, react in the reactor at 200°C for 18 hours, cool After reaching room temperature, centrifuge and wash three times with ultrapure water and absolute ethanol, and then vacuum-dry at 50°C; then, put the ground powder into a muffle furnace with a heating rate of 3°C / min, and calcinate at 560°C for 10 min ; Finally, the calcined powder is cooled to room temperature to obtain FeCo-NTiO 2 ;

[0046] Described iron salt is ferric nitrate;

[0047] Described cobalt salt is cobalt nitrate;

[0048] Described ammonium salt is ammonium nitrate.

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Abstract

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical diethylstilbestrol sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Diethylstilbestrol is a synthetic non-steroidal estrogen substance. At present, the methods for detecting diethylstilbestrol mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need profes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 张勇杜斌马洪敏吴丹范大伟
Owner UNIV OF JINAN
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