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Fabrication method and application of tetracycline antibiotic molecular imprinting electrochemical sensor

A technology of molecular imprinting and antibiotics, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of reduced sensitivity of electrochemical sensors, limiting the application of molecularly imprinted membranes, and reducing stability and reproducibility, and achieves excellent electronic performance. The effect of transmission ability, lower testing cost and convenient operation

Active Publication Date: 2019-01-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electrochemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

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  • Fabrication method and application of tetracycline antibiotic molecular imprinting electrochemical sensor
  • Fabrication method and application of tetracycline antibiotic molecular imprinting electrochemical sensor
  • Fabrication method and application of tetracycline antibiotic molecular imprinting electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of NiFeN-nanoarray

[0031] (1) The disposable disposable electrode is ultrasonically cleaned with dilute hydrochloric acid, anhydrous ethanol and deionization to remove the oxide layer and surface impurities of the disposable disposable electrode;

[0032] (2) Weigh 1 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 A mixture of 3 mmol urea CO (NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water, stir until it becomes clear, and then transfer it to a 50 mL polytetrafluoroethylene reactor;

[0033] (3) Putting the disposable disposable electrode processed in step (1) into the solution in the reaction kettle in step (2), and reacting at a temperature of 100 ° C for 12 hours to prepare nickel-iron bimetallic layered hydrogen oxide nanosheet array precursor electrode;

[0034] (4) Insert the nickel-iron bimetallic hydroxide nanosheet array precursor electrode obtained in step (3) into a phosphate buffer solution PBS containing dopamine, ammoniu...

Embodiment 2

[0036] Example 2 Preparation of NiFeN-nanoarray

[0037] (1) The disposable disposable electrode is ultrasonically cleaned with dilute hydrochloric acid, anhydrous ethanol and deionization to remove the oxide layer and surface impurities of the disposable disposable electrode;

[0038] (2) Weigh 2 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 A mixture of 6 mmol urea CO (NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water, stir until it becomes clear, and then transfer it to a 50 mL polytetrafluoroethylene reactor;

[0039] (3) put the disposable disposable electrode processed in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 110 ° C for 11 hours to prepare nickel-iron bimetallic layered hydrogen oxide nanosheet array precursor electrode;

[0040] (4) Insert the nickel-iron bimetallic hydroxide nanosheet array precursor electrode obtained in step (3) into a phosphate buffered solution PBS containing dopamine, ammonium per...

Embodiment 3

[0042] Example 3 Preparation of NiFeN-nanoarray

[0043] (1) The disposable disposable electrode is ultrasonically cleaned with dilute hydrochloric acid, anhydrous ethanol and deionization to remove the oxide layer and surface impurities of the disposable disposable electrode;

[0044] (2) Weigh 3 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 A mixture of 9 mmol urea CO (NH 2 ) 2 , put it into a 50 mL beaker, add 30 mL of deionized water, stir until it becomes clear, and then transfer it to a 50 mL polytetrafluoroethylene reactor;

[0045] (3) Put the disposable disposable electrode processed in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 130 ° C for 9 hours to prepare nickel-iron bimetallic nitride nanometers Sheet array precursor electrodes;

[0046] (4) Insert the nickel-iron bimetallic hydroxide nanosheet array precursor electrode obtained in step (3) into a phosphate buffered solution PBS containing dopamine, ammonium persulfate ...

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Abstract

The invention discloses a fabrication method of a tetracycline antibiotic molecular imprinting electrochemical sensor, and belongs to the technical field of novel nanometer functional material and chemical biological sensor. A nickel-iron thermometal nitride nanosheet array is fabricated on a disposable throwable electrode, an electronic medium-containing polydopamine thin film and a molecular imprinting polymer taking ampicillin as a template molecule polymer are directly and sequentially fabricated on the nickel-iron thermometal layered hydroxide nanosheet array by an in-situ growth method according to large specific surface area, high-activity hydroxyl functional group and amino functional group of polydopamine. After the template molecule is eluted, the original position of the template molecule is changed to holes, and the molecule imprinting polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic molecular imprinting electrochemical sensor is fabricatedand completed.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemical analysis sensor. It belongs to the technical field of novel nanometer functional materials and biosensing analysis. Background technique [0002] Tetracyclic antibiotics, also known as ampicillins, are beta-lactam antibiotics, semi-synthetic broad-spectrum penicillins that can treat a variety of bacterial infections. Indications include respiratory tract infections, urinary tract infections, meningitis, salmonella infections, and endocarditis. Because of its convenient use and low cost, it is mostly used to treat infectious diseases caused by sensitive bacteria in chickens, such as Escherichia coli, Salmonella, Pasteurella, Staphylococcus and Streptococcus infections. On October 27, 2017, in the list of carcinogens published by the International Agency for Research on Cancer of the World Health Organization, ampicillin was included in the list of 3 types of carcinogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 张勇刘召壹杜斌
Owner UNIV OF JINAN
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