Preparation method for tetracycline antibiotic electrochemiluminescence sensor, and application

A technology of electrochemistry and antibiotics, which is applied in the direction of chemiluminescence/bioluminescence, analysis by causing chemical reactions of materials, and electrochemical variables of materials, etc., which can solve the problems of decreased sensitivity, stability and reproducibility of electrochemical sensors, Limit the application of molecularly imprinted membranes and other problems, achieve excellent electron transfer capabilities, convenient operation, and reduce detection costs

Active Publication Date: 2019-01-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preparation process of traditional MIP-ECS, there are disadvantages such as difficult elution of template molecules, difficulty in controlling the thickness of imprinted membranes, and poor reproducibility, which limit the application of molecularly imprinted membranes in electroanalytical chemical sensors.
These problems, especially the difficult control of the thickness of the molecularly imprinted membrane, which leads to the decrease of the sensitivity of the electrochemical sensor, and the technical difficulties that the molecularly imprinted membrane is easily detached from the electrode surface during the elution process, resulting in a decrease in stability and reproducibility, limit the application of MIP_ECS. It is of great research significance and market value to find new synthesis methods for molecularly imprinted polymers, new methods for modifying molecularly imprinted membrane electrodes, and methods for combining molecularly imprinted membranes with substrate materials to solve the problems of preparation and application of MIP-ECS.

Method used

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  • Preparation method for tetracycline antibiotic electrochemiluminescence sensor, and application
  • Preparation method for tetracycline antibiotic electrochemiluminescence sensor, and application
  • Preparation method for tetracycline antibiotic electrochemiluminescence sensor, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of NiFeN-nanoarray

[0031] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0032] (2) Weigh 1 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 3 mmol urea CO(NH 2 ) 2 , put it into a 50mL beaker, add 30mL deionized water and stir until clear, then transfer to a 50mL polytetrafluoroethylene reactor;

[0033] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 100°C for 12 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0034] (4) Insert the nickel-iron bimetallic layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine and ammonium persulfate, and react at a temperature of 20 °C for ...

Embodiment 2

[0036] Example 2 Preparation of NiFeN-nanoarray

[0037] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0038] (2) Weigh 2 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 6 mmol urea CO(NH 2 ) 2 , put it into a 50mL beaker, add 30mL deionized water and stir until clear, then transfer to a 50mL polytetrafluoroethylene reactor;

[0039] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 110°C for 11 hours to prepare nickel-iron bimetallic layered hydrogen Oxide nanosheet array precursor electrode;

[0040] (4) Insert the nickel-iron bimetallic layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine and ammonium persulfate, and react at a temperature of 30 °C for ...

Embodiment 3

[0042] Example 3 Preparation of NiFeN-nanoarray

[0043] (1) Use dilute hydrochloric acid, absolute ethanol and deionized disposable electrode for ultrasonic cleaning to remove the oxide layer and surface impurities of the disposable electrode;

[0044] (2) Weigh 3 mmol Ni(NO 3 ) 2 and Fe(NO 3 ) 3 mixture and 9 mmol urea CO(NH 2 ) 2 , put it into a 50mL beaker, add 30mL deionized water and stir until clear, then transfer to a 50mL polytetrafluoroethylene reactor;

[0045] (3) Put the disposable disposable electrode treated in step (1) into the solution in the reaction kettle in step (2), and react at a temperature of 130°C for 9 hours to prepare a nickel-iron double metal nitride nanometer Sheet array precursor electrode;

[0046] (4) Insert the nickel-iron bimetallic layered hydroxide nanosheet array precursor electrode obtained in step (3) into the phosphate buffer solution PBS containing dopamine and ammonium persulfate, and react at a temperature of 40 °C for 6 hour...

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Abstract

The invention discloses a preparation method for a tetracycline antibiotic electrochemiluminescence sensor, and belongs to the technical field of novel nanometer function materials and biosensing analysis. The preparation method firstly prepares a nickel-iron bimetallic oxide nanosheet array on a disposable electrode, the large specific surface area of the nickel-iron bimetallic oxide nanosheet array, high adsorption activity for an amino and the amino functional group of polydopamine are used, an in-situ growth method is adopted to successively directly preparing a polydopamine film and a molecularly imprinted polymer which coats luminol in an in-situ way and takes tetracycline antibiotics as a template molecule on the nickel-iron bimetallic oxide nanosheet array, and after the template molecule is eluted, the position of the original template molecule becomes a cavity, i.e., the molecularly imprinted polymer of the template molecule is eluted. Therefore, the tetracycline antibiotic electrochemiluminescence sensor finishes being prepared.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemiluminescence sensor. It belongs to the field of new nano functional material and biosensing analysis technology. Background technique [0002] Tetracyclic antibiotics, also known as ampicillin, is a β-lactam antibiotic, a semi-synthetic broad-spectrum penicillin, which can treat a variety of bacterial infections. Indications include respiratory tract infection, urinary tract infection, meningitis, salmonella infection, and endocarditis. Because of its convenient use and low cost, it is mostly used to treat infectious diseases caused by sensitive bacteria in chickens, such as Escherichia coli, Salmonella, Pasteurella, Staphylococcus and Streptococcus infections. On October 27, 2017, in the list of carcinogens released by the International Agency for Research on Cancer of the World Health Organization, ampicillin was included in the list of category 3 carcinogens. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76
CPCG01N21/76G01N27/26G01N27/30G01N27/3278
Inventor 张勇程荣琦杜斌
Owner UNIV OF JINAN
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