A preparation method of electrochemical biosensor based on iron-doped two-dimensional nanomaterials

A technology of two-dimensional nanomaterials and biosensors, which is applied in the field of preparation of electrochemical biosensors, can solve the problems of low yield and cumbersome process, and achieve the effects of low cost, simple preparation and convenient operation

Inactive Publication Date: 2019-03-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Fe-TiO 2 / MoS 2 preparation of

[0040] (1) Add 0.6 g of molybdenum disulfide powder and 0.2 mmol of iron salt to 3 mL of n-butyllithium solution, and stir for 12 hours under nitrogen protection at 60 °C to obtain the reacted solution;

[0041] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 60 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain iron Intercalated molybdenum disulfide nanomaterials;

[0042] (3) Add 500 mg of the iron-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, and after stirring for 1 hour, slowly add 0.5 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 160°C for 18 hours;

[0043] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-d...

Embodiment 2

[0048] Example 2 Fe-TiO 2 / MoS 2 preparation of

[0049] (1) Add 0.6 g of molybdenum disulfide powder and 1.0 mmol of iron salt to 5 mL of n-butyllithium solution, and stir for 24 hours under nitrogen protection at 30 °C to obtain the reacted solution;

[0050] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 30 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain iron Intercalated molybdenum disulfide nanomaterials;

[0051] (3) Add 200 mg of the iron-intercalated molybdenum disulfide nanomaterials prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, slowly add 0.6 mL of hydrofluoric acid while stirring, and then Reaction in the reactor at 180°C for 20 hours;

[0052] (4) The reaction product obtained in step (3) was centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50 °C ...

Embodiment 3

[0057] Example 3 Preparation method of electrochemical biosensor

[0058] (1) Using ITO conductive glass as the working electrode, drop-coat 8 µL of Fe-TiO on the surface of the electrode 2 / MoS 2 Sol, dry at room temperature;

[0059] (2) Wash the electrode obtained in step (1) with the buffer solution PBS, continue to drop-coat 8 µL of 10 µg / mL citrinin antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0060] (3) Wash the electrode obtained in step (2) with PBS, continue to drip-coat 8 µL of bovine serum albumin solution with a concentration of 100 µg / mL on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0061] (4) Wash the electrode obtained in step (3) with PBS, store it in a refrigerator at 4°C and dry it, and then prepare the electrochemical biosensor;

[0062] The Fe-TiO 2 / MoS 2 The sol is 50 mg of Fe-TiO 2 / MoS 2 The powder was dissolved in 10 mL ultrapure water, and the hydrosol o...

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Abstract

The invention discloses a preparation method of an electrochemical biosensor. The present invention first prepares a novel two-dimensional nano-electrode material FeTiO2 / MoS2, that is, a two-dimensional nano-composite material in which iron-doped titanium dioxide nano-squares are in-situ compounded with molybdenum disulfide, and utilizes the good biocompatibility and Large specific surface area, loaded with citrinin antibody, during detection, because iron-doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ, generate electrochemical signal, and then use the specific quantitative combination of antibody and antigen to determine the electron transport ability Therefore, the current intensity is reduced accordingly, and the construction of a biosensor for detecting citrinin by a label-free electrochemical method is finally realized.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Mycotoxins mainly refer to the toxic metabolites produced by molds in their contaminated food, which can enter human and animal bodies through feed or food, causing acute or chronic toxicity to humans and animals, and damage the body's liver, kidneys, and nervous tissues , hematopoietic tissue and skin tissue. Mycotoxins can form when crops are harvested in the field; under unfavorable storage conditions, mycotoxins can also continue to form on crops after harvest. Higher humidity generally favors the growth of mold and the production of mycotoxins in the feed. Temperature is another important factor. Crops in hot and dry environments are vulnerable to mold spores, which can produce mycotoxins when conditions permit. [0003] There are many kinds of myco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 张勇庞雪辉李燕王晓东魏琴
Owner UNIV OF JINAN
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