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Preparation method and application of electrogenerated chemiluminescence furazolidone biosensor

A biosensor, furazolidone technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, scientific instruments, etc., can solve the problems of low sensitivity of electrochemiluminescence sensor, unfavorable practical application, poor conductivity, etc. , to increase the intensity of electrochemiluminescence, improve the intensity and stability, and solve the effect of poor conductivity

Inactive Publication Date: 2016-06-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemiluminescence sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Mn-TiO 2 / g -C 3 N 4 preparation of

[0036] First, take 0.8mmol manganese salt and add it to 5mL tetrabutyl titanate. During the stirring process, slowly add 0.5mL hydrofluoric acid, and react in the reactor at 160°C for 24 hours. After cooling to room temperature, use ultrapure water and After centrifugal washing with absolute ethanol for three times, vacuum-dry at 50°C; secondly, mix 150mg of the dried solid with 400mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace with a heating rate of 1°C / min, calcined at 480°C for 5 hours; finally, the calcined powder was cooled to room temperature to obtain Mn-TiO 2 / g -C 3 N 4 ;

[0037] Described manganese salt is manganese sulfate.

Embodiment 2

[0038] Example 2 Mn-TiO 2 / g -C 3 N 4 preparation of

[0039] First, take 1.0 mmol of manganese salt and add it to 5 mL of tetrabutyl titanate. During the stirring process, slowly add 0.6 mL of hydrofluoric acid, and react in the reactor at 180 ° C for 21 hours. After cooling to room temperature, use ultrapure water and After centrifugal washing with absolute ethanol for three times, vacuum-dry at 50°C; secondly, mix 200mg of the dried solid with 400mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace with a heating rate of 2°C / min, calcined at 520°C for 2 hours; finally, the calcined powder was cooled to room temperature to obtain Mn-TiO 2 / g -C 3 N 4 ;

[0040] Described manganese salt is manganese chloride.

Embodiment 3

[0041] Example 3 Mn-TiO 2 / g -C 3 N 4 preparation of

[0042] First, take 1.2 mmol of manganese salt and add it to 5 mL of tetrabutyl titanate. During the stirring process, slowly add 0.8 mL of hydrofluoric acid, and react in the reactor at 200 ° C for 18 hours. After cooling to room temperature, use ultrapure water and After centrifugal washing with absolute ethanol for three times, vacuum-dry at 50°C; secondly, mix 250mg of the dried solid with 400mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace with a heating rate of 3°C / min, calcined at 560°C for 0.5 hours; finally, the calcined powder was cooled to room temperature to obtain Mn-TiO 2 / g -C 3 N 4 ;

[0043] Described manganese salt is manganese nitrate.

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Abstract

The invention discloses a preparation method of an electrogenerated chemiluminescence furazolidone biosensor and belongs to the technical field of novel nano functional materials and biological sensors. A novel two-dimensional nano composite material Mn-TiO2 / g-C3N4 is prepared first, the material is loaded with furazolidone antibodies by means of good biocompatibility and large specific surface area, then by means of the cross-linking effect of glutaraldehyde, horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen enzyme in situ in detection, an electrochemical reaction is performed on a base solution, a high and stable electrogenerated chemiluminescence signal is generated, by means of influences of specific quantitative combination of antibodies and antigens on electron transmission capacity, the electrogenerated chemiluminescence signal is correspondingly reduced, finally the low-cost, high-sensitivity and good-specificity electrogenerated chemiluminescence biosensor for detecting furazolidone is prepared, and the biosensor is fast in detection and easy to prepare.

Description

Technical field [0001] The present invention involves a preparation method for electrochemical chemical glowing furazolidone biosensor.It belongs to the new type of nano -functional materials and biosensor technology. Background technique [0002] Aurazolidone (Di Ling) is a nitro -amura antibiotic, which is a broad -spectrum antibacterial drug.When used as veterinary drugs, amuoloone has good effects on preventing and treating certain raw diseases, water mold, bacterial gills, red dermatology, and hemorrhage disease.In the breeding industry, amurazolidone can be used to treat livestock and poultry intestinal infections, such as piglets yellow and white diarrhea.In the water industry, amuoloone has a certain effect on salmonia infection brain adhesion.However, the Ministry of Agriculture in my country lists amuolorazolidone as prohibited drugs and cannot be detected in animal food.The FDA also banned the use of nitro -amu) in animal food in animal food in 2002. [0003] At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/5438
Inventor 张勇庞雪辉李燕王晓东魏琴
Owner UNIV OF JINAN
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