Preparing method and application of electrochemiluminescence carbamate sensor based on cobalt-doped two-dimensional nanometer composite

A carbamate, two-dimensional nanotechnology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., can solve the problems of poor conductivity, unfavorable practical application, and low sensitivity of electrochemiluminescence sensors. , to improve the strength and stability, improve the resonance energy transfer ability, and increase the intensity of electrochemiluminescence

Inactive Publication Date: 2016-07-06
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemiluminescence sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1Co-MoO 3 / TiO 2 g-C 3 N 4 preparation of

[0036] First, add 0.6mmol sodium molybdate and 0.8mmol cobalt salt into 5mL tetrabutyl titanate, slowly add 0.5mL hydrofluoric acid during stirring, react in the reactor at 160°C for 24 hours, and cool to room temperature , after centrifuging and washing three times with ultrapure water and absolute ethanol, vacuum-dry at 50°C; secondly, take 150 mg of the dried solid and mix it with 400 mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace , the heating rate was 1°C / min, and calcined at 480°C for 5 hours; finally, the calcined powder was cooled to room temperature to obtain Co-MoO 3 / TiO 2 g-C 3 N 4 ;

[0037] The cobalt salt is cobalt sulfate.

Embodiment 2

[0038] Example 2Co-MoO 3 / TiO 2 g-C 3 N 4 preparation of

[0039] First, add 0.8mmol sodium molybdate and 1.0mmol cobalt salt to 5mL tetrabutyl titanate, slowly add 0.65mL hydrofluoric acid during stirring, react in the reactor at 180°C for 21 hours, and cool to room temperature , washed with ultrapure water and absolute ethanol for three times, then vacuum-dried at 50°C; secondly, take 200mg of the dried solid and mix it with 400mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace , the heating rate is 2°C / min, and calcined at 520°C for 2 hours; finally, the calcined powder is cooled to room temperature to obtain Co-MoO 3 / TiO 2 g-C 3 N 4 ;

[0040] Described cobalt salt is cobalt chloride.

Embodiment 3

[0041] Example 3Co-MoO 3 / TiO 2 g-C 3 N 4 preparation of

[0042] First, add 1.0mmol sodium molybdate and 1.2mmol cobalt salt into 5mL tetrabutyl titanate, slowly add 0.8mL hydrofluoric acid during stirring, react in the reactor at 200°C for 18 hours, and cool to room temperature , after centrifuging and washing with ultrapure water and absolute ethanol three times, vacuum drying at 50°C; secondly, take 250 mg of the dried solid and mix it with 400 mg of melamine, and grind it into powder; then, put the ground powder into a muffle furnace , the heating rate was 3°C / min, and calcined at 560°C for 0.5 hours; finally, the calcined powder was cooled to room temperature to obtain Co-MoO 3 / TiO 2 g-C 3 N 4 ;

[0043] The cobalt salt is cobalt nitrate.

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Abstract

The invention discloses a preparing method of an electrochemiluminescence carbamate sensor based on a cobalt-doped two-dimensional nanometer composite, and belongs to the technical field of novel nanometer functional materials and biosensors. The preparing method comprises the steps that firstly, a novel two-dimensional manometer material Co-MoO3/TiO2@g-C3N4 is prepared, and a carbamate antibody is loaded by using the good biocompatibility and the large specific area of the material; secondly, horse radish peroxidase is fixed through the cross-linking action of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ and is subjected to an electrochemical reaction with a base solution to generate high and stable electrochemiluminescence signals, then the influence on the electron transmittability by the specific and quantitative combination of the antibody and an antigen is used, the electrochemiluminescence signals are reduced correspondingly, and finally the carbamate detecting electrochemiluminescence biosensor low in cost, high in sensitivity and specificity, fast in detection and easy to prepare is obtained.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescence carbamate sensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Carbamate is a compound in which the amino group or amine group is directly linked to the carbonyl of the formate ester, and is used as a pesticide, herbicide, fungicide, etc. Carbamate pesticides are the main cause of acute pesticide poisoning, and they are also the key detection varieties of pesticide residues in vegetables nowadays, because the specific poisoning symptoms of carbamate pesticides begin to appear after 3 hours of continuous work, and the initial poisoning symptoms are those who are poisoned. Feeling unwell and possibly vomiting, nausea, headache and dizziness, fatigue and chest tightness; later the patient begins to sweat profusely and salivate (drool), blurred vision, spontaneous muscle contractions, convulsions, tachycardi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 张勇孙旭魏琴李燕马洪敏
Owner UNIV OF JINAN
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