Method and kit for detecting fentanyl/morphine compounds

A compound, kit technology, applied in the field of analytical chemistry

Pending Publication Date: 2020-08-11
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on SERS detection of fentanyl / morphine compounds, and SERS detection in simple matrices such as aqueous solution and methanol solution is the main one (Anal.Chem.2018, 90, 12678–12685; J.Light Scatter, 2010, 22, 34-38.)

Method used

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  • Method and kit for detecting fentanyl/morphine compounds
  • Method and kit for detecting fentanyl/morphine compounds
  • Method and kit for detecting fentanyl/morphine compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] The preparation of embodiment 1AuNPs

[0197] 1.1 AuNPs with a particle size of 50-55nm were prepared by reducing chloroauric acid with trisodium citrate.

[0198] Weigh 100mg tetrachloroauric acid trihydrate into a 100mL volumetric flask, dissolve with ultrapure water and make to volume to obtain 1mg / mL mother solution. Measure 10mL of the mother liquor and dilute it to 100mL with ultrapure water, transfer it to a 250mL three-necked round bottom flask for oil bath, set the temperature at 110°C and the speed at 1100r / min, heat to boiling and quickly add 0.84mL mass The aqueous solution of trisodium citrate dihydrate with a fraction of 0.1% was continuously heated and stirred for 40 min, then the heat source was removed, cooled naturally to room temperature, then packaged and stored at room temperature to obtain AuNPs.

[0199] The prepared AuNPs were characterized by UV-Vis spectroscopy and transmission electron microscopy, as Figure 2-A and Figure 2-B As shown, th...

Embodiment 2

[0203] Raman and SERS spectrograms of embodiment 2 fentanyl / morphine compounds

[0204] In this embodiment, the analytes include fentanyl compounds: fentanyl, remifentanil, 3-methylfentanyl, carfentanil, sufentanil; and morphine compounds: morphine, 6- Monoacetylmorphine, heroin, codeine, thienorphine, 030418. The structures of the above-mentioned fentanyl / morphine compounds are as follows: Figure 3-A and Figure 3-B shown.

[0205] Take 200 μL of an aqueous solution with a concentration of 500 ng / mL of the analyte, and at the same time take 200 μL of ultrapure water as a blank sample. Add concentrated AuNPs to the analyte aqueous solution or blank sample, so that the concentration of AuNPs in the test sample is about 0.25nM, mix well, then add NaCl aqueous solution, make the concentration of NaCl in the test sample about 200mM, mix well, use a portable The Raman spectrometer was used for SERS detection, the laser power was set to 60mW, the integration time was set to 10s...

Embodiment 3

[0206] Embodiment 3 different kinds of inorganic salts to fentanyl 1000cm -1 SERS peak and codeine at 622cm -1 Influence of SERS peak intensity at

[0207] In this embodiment, fentanyl is used as a representative of fentanyl compounds, and codeine is used as a representative of morphine compounds. Take 9 parts of aqueous fentanyl solution with a concentration of 100 ng / mL and 9 parts of an aqueous solution of codeine with a concentration of 100 ng / mL, each with a volume of about 200 μL. Add concentrated AuNPs to each sample, so that the concentration of AuNPs in each test sample is about 0.25nM, mix well, and then add NaCl, KCl, MgCl to 9 samples of fentanyl aqueous solution 2 、Na 2 SO 4 、K 2 SO 4 , MgSO 4 , NaI, KI, MgI 2 Aqueous solution, add NaCl, KCl, MgCl respectively to 9 parts of codeine aqueous solution samples 2 、Na 2 SO 4 、K 2 SO 4 , MgSO 4 , KI, NaI, MgI 2 Aqueous solution, so that NaCl, KCl, MgCl in each test sample 2 、Na 2 SO 4 、K 2 SO 4 , MgSO...

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Abstract

The invention relates to a method for detecting fentanyl compounds / morphine compounds in a sample, and further relates to a kit and application of the kit to detection of fentanyl compounds / morphine compounds in the sample. The method can be used for quickly and qualitatively detecting whether a sample contains fentanyl compounds and / or morphine compounds or not, and further can be used for quantitatively detecting trace fentanyl compounds in the sample. The method has advantages of sensitivity, quickness, easiness in operation and the like, and can be applied to the fields of on-site quick detection, poison detection, forensic identification and the like.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, relates to a method for detecting fentanyl compounds / morphine compounds in a sample, and also relates to a kit and the kit used for detecting fentanyl compounds / morphine compounds in samples use. Background technique [0002] Both fentanyl and morphine are opioid receptor agonists, which are commonly used clinically to relieve pain in cancer patients and other chronic pain. Currently, more than 1400 fentanyl congeners and 250 morphine congeners have been reported (Forensic Toxicol., 2018, 36, 12-32; https: / / en.wikipedia.org / wiki / Morphine). Some have medicinal uses, among which the fentanyl homologue carfentanil has the strongest analgesic activity, and its analgesic ability is 100 times higher than that of fentanyl (Arzneimittelforschung, 1976, 26, 1521-1531), while fentanyl Its analgesic ability is 50-100 times that of morphine. In addition, fentanyl and morphine-like compounds can also pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 郭磊王凯徐斌吴剑峰朱颖洁谢剑炜
Owner ACADEMY OF MILITARY MEDICAL SCI
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