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Method for preparing magnetic DNA (deoxyribonucleic acid) supramolecular hydrogel and application thereof

A supramolecular hydrogel and magnetic technology is applied in the field of biomedicine to achieve the effects of overcoming cumbersome steps, simple preparation method and easy operation.

Active Publication Date: 2017-02-15
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are no relevant literature reports on the preparation of magnetic DNA supramolecular hydrogels and their use in the construction of drug carriers and ATP detection systems

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  • Method for preparing magnetic DNA (deoxyribonucleic acid) supramolecular hydrogel and application thereof
  • Method for preparing magnetic DNA (deoxyribonucleic acid) supramolecular hydrogel and application thereof
  • Method for preparing magnetic DNA (deoxyribonucleic acid) supramolecular hydrogel and application thereof

Examples

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Embodiment 1

[0146] A preparation method of magnetic DNA supramolecular hydrogel. Experimental principle such as figure 1 As shown, first, three single strands (Y1, Y2, Y3) are used to construct a Y-shaped DNA monomer (Y-DNA), and two single strands (L1, L2) are used to construct a linker DNA monomer (L-DNA); at the same time , using the amide reaction between graphene oxide and amino magnetic beads to prepare magnetic graphene oxide. DNA supramolecular hydrogels were formed by complementary hybridization self-assembly of Y-DNA and L-DNA sticky ends. Based on the π-π stacking interaction between single-stranded DNA and magnetic graphene oxide, the DNA hydrogel was passed through the exposed sticky ends. Adsorbed on the surface of magnetic graphene oxide, a magnetic DNA supramolecular hydrogel was prepared.

[0147] Concrete preparation method is as follows:

[0148] 1. Preparation of Magnetic Graphene Oxide

[0149] Put a certain amount of graphene oxide sheets in a beaker, add seconda...

Embodiment 2

[0155] A technical application of the magnetic DNA supramolecular hydrogel as a drug carrier. principle such as figure 2 As shown, based on the self-assembly of Y-DNA and L-DNA cross-linking to form DNA supramolecular hydrogel, combined with the adsorption of magnetic graphene oxide to single-stranded DNA, the DNA is bound to the exposed sticky ends of the DNA hydrogel. The magnetic DNA supramolecular hydrogel is prepared by adsorbing the supramolecular hydrogel on the surface of the magnetic graphene oxide, which is the magnetic DNA supramolecular hydrogel prepared in the first aspect of this patent. Furthermore, using the property that the anti-tumor drug doxorubicin (DOX) can be intercalated into the DNA double-strand structure, the anti-tumor drug DOX was embedded into the DNA double-strand structure of the magnetic DNA supramolecular hydrogel to prepare the Magnetic DNA supramolecular hydrogel drug carrier loaded with anti-tumor drug DOX. Since deoxyribonuclease I (Deo...

Embodiment 3

[0170] A magnetic DNA supramolecular hydrogel coated with horseradish peroxidase (HRP), which realizes the application system of high sensitivity and high selectivity detection of adenosine triphosphate (ATP). principle such as Figure 5 As shown, the partial sequence of L1 in L-DNA was designed as an ATP aptamer, and other DNA sequences were changed to prepare two DNA monomers (Y-DNA' and L-DNA'). The cross-linking effect of Y-DNA' and L-DNA' is used to form DNA hydrogel, and HRP is coated in DNA hydrogel at the same time. Further based on the π-π stacking interaction between single-stranded DNA and magnetic graphene oxide, HRP-coated DNA hydrogel was adsorbed on the surface of magnetic graphene oxide through the exposed sticky ends to prepare HRP-coated magnetic DNA supramolecular water. gel. Based on the specific recognition of the nucleic acid aptamer and the target, when there is ATP, the ATP specifically binds to its aptamer chain to form an ATP-aptamer complex, which ...

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Abstract

The invention relates to a method for preparing magnetic DNA (deoxyribonucleic acid) supramolecular hydrogel and application thereof. The magnetic DNA supramolecular hydrogel comprises magnetic oxidized graphene and two types of DNA monomers [Y-type DNA monomers (Y-DNA) and linker DNA monomers (L-DNA)]. The Y-DNA and the L-DNA are hybridized by sticky ends in a complementary manner to form DNA hydrogel; exposed sticky ends of the DNA hydrogel are adsorbed on the surfaces of the magnetic oxidized graphene under pi-pi stacking effects to form the magnetic DNA supramolecular hydrogel. The method and the application have the advantages that the magnetic DNA supramolecular hydrogel prepared by the aid of the method is low in cost, easy to operate and separate and high in reaction speed and universality; the method can be successfully applied to colorimetry and ultraviolet-visible absorption quantitative detection on medicine carriers and adenosine triphosphate (ATP); as compared with existing detection methods, the method is easy and convenient to implement and low in cost and has a broad application prospect, visual detection results can be obtained, and complicated instruments or equipment can be omitted.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a preparation method and application of a magnetic DNA supramolecular hydrogel. Background technique [0002] Nanomaterials refer to materials with at least one dimension of the three-dimensional space of the particles at the nanoscale (usually 0.1-100 nm). Magnetic nanomaterials are an important class of nanomaterials, which have the following characteristics: (1) The material is small in size and can freely penetrate blood vessels; (2) It can produce corresponding thermal effects with the change of the external magnetic field; (3) It can be functionalized according to the purpose of use. , enhance its biocompatibility and targeting; (4) The material exhibits special physical and chemical properties such as quantum size effect, surface effect, macroscopic quantum tunneling effect and good biocompatibility, and can be used as a magnetic carrier for drugs transmission etc. In recent y...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08K9/04C08K3/04G01N33/573A61K9/06A61K47/26A61K47/04A61K31/704
CPCA61K9/0009A61K9/06A61K31/704A61K47/02A61K47/26C08J3/075C08J3/24C08K3/04C08K9/04C08K2201/01G01N33/5735
Inventor 毕赛岳淑珍王宗花
Owner QINGDAO UNIV
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