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Preparation method of aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR

A nucleic acid aptamer and MC-LR technology, which is applied in the field of photoelectrochemical analysis technology and environmental detection, can solve the problems of time-consuming analysis, high instrument price, and difficulty in achieving selective analysis, achieving high sensitivity and selectivity, and low price. Inexpensive, Ultra Sensitive and Selective Effects

Inactive Publication Date: 2015-09-09
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a nucleic acid aptamer photoelectric based on highly sensitive and selective detection of MC-LR for the high price of the existing analysis technology of MC-LR, the time-consuming analysis and the difficulty in realizing the defects of selective analysis. Preparation method of chemical sensor

Method used

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  • Preparation method of aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR
  • Preparation method of aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR

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Experimental program
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Effect test

Embodiment 1

[0028] Construction of a highly sensitive and highly selective nucleic acid aptamer photoelectrochemical sensor for detecting water environmental pollutants MC-LR, using anodic oxidation method to prepare titanium dioxide nanotubes on titanium substrates, and modify cadmium sulfide nanoparticles to form shell polymers Sugar and glutaraldehyde are used to functionalize the surface of the electrode to construct a photoelectric biological interface, and chemically bond the MC-LR nucleic acid aptamer with a specific base sequence to the electrode surface to prepare the MC-LR nucleic acid aptamer photoelectrochemical sensor , the specific preparation process is as follows:

[0029] (1) The titanium plate is mechanically polished with sandpaper of different meshes in sequence until the surface of the titanium plate becomes a smooth mirror surface, and then placed in deionized water, ethanol, and deionized water solutions for ultrasonic cleaning for 15 minutes. Take out the titanium ...

Embodiment 2

[0035] (4) The prepared nucleic acid aptamer photoelectrochemical sensor was used as the working electrode, the saturated calomel electrode was used as the reference electrode, the platinum electrode was used as the counter electrode, and the PBS buffer containing 0.1 mol / L ascorbic acid was used as the electrolyte. Apply 0.0V bias voltage under visible light irradiation, and measure the i~t curve of the system after adding different concentrations of MC-LR continuously. Since the aptamer can specifically recognize MC-LR, when MC-LR is added to the electrolyte, MC-LR diffuses to the electrode surface to form a complex of MC-LR-aptamer, which prevents the ascorbic acid in the electrolyte from moving to the electrode surface. The electrode surface diffuses, thereby causing a decrease in photocurrent. Using the linear relationship between the photocurrent reduction value ΔI and the logarithm of the concentration of MC-LR, a working curve is established to realize the quantitative...

Embodiment 3

[0037] The prepared nucleic acid aptamer photoelectrochemical sensor was used as the working electrode, the saturated calomel electrode was used as the reference electrode, the platinum electrode was used as the counter electrode, and the PBS buffer containing 0.1 mol / L ascorbic acid was used as the electrolyte, and 0.0 V bias voltage, using the i~t curve method to measure the photocurrent of the mixed electrolyte solution added with MC-LR solution and 100 concentration of interfering substances in MC-LR. The selected interfering substances were glyphosate, tetrafenazine, atrazine, trichlorfon, acetamiprid, paraquat, omethoate, and monosultap. The results showed that when the concentration of interfering substances was 100 times that of MC-LR, the interference on the photocurrent of MC-LR was less than 5%. It can be seen that since the nucleic acid aptamer can specifically recognize the MC-LR molecule, the prepared nucleic acid aptamer photoelectrochemical sensor shows high se...

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Abstract

The invention relates to a preparation method of an aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR. The preparation method comprises preparing titanium dioxide nanotubes on a titanium matrix by an anodization method, carrying out modification with cadmium sulfide nanoparticles, functionally assembling chitosan and glutaraldehyde to the surface of an electrode so that a photoelectric biological interface is built, and bonding a MC-LR aptamer with a specific base sequence to the surface of the base electrode by chemical bonding so that the MC-LR aptamer photoelectrochemical sensor is prepared. Through combination of a supersensitive photoelectric analysis technology and an aptamer technology with a MC-LR specific recognition function, the preparation method realizes high-sensitivity high-selectivity analysis detection of MC-LR, and has the MC-LR detection limit of 5.3*10<-12> mol / L and a linear detection range of 7.2*10<-8> to 8*10<-12> mol / L. Compared with the prior art, the preparation method has the advantages of simple processes, fast response and low cost and can realize high-sensitivity high-selectivity analysis of MC-LR.

Description

technical field [0001] The invention belongs to the field of photoelectrochemical analysis technology and environmental detection technology, and relates to a preparation method of a nucleic acid aptamer photoelectrochemical sensor for detecting MC-LR with high sensitivity and high selectivity. Background technique [0002] Microcystin-LR (MC-LR) is a cyclic heptapeptide water environmental pollutant released by cyanobacteria during the reproduction process in eutrophic water rich in N and P elements. Due to the existence of the ring structure and spacer double bonds in the molecule, MC-LR is chemically stable and difficult to degrade under natural conditions; MC-LR is highly toxic, and very low concentrations of MC-LR can inhibit protein phosphatases PP1 and PP2A The activity of liver cells causes the decomposition and rupture of liver cells, leading to liver cell dysfunction, and shows strong hepatotoxicity and tumor-promoting effects on both humans and animals. In recent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 赵国华李修艳
Owner TONGJI UNIV
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