Preparation and application of unmarked electrochemiluminescent tumor marker immunosensor

A technology of tumor markers and immune sensors, which is applied in the field of new nano-functional materials and biosensors, can solve the problems of unfavorable light-emitting systems and harsh reaction conditions, achieve important scientific significance and application value, and improve sensitivity and accuracy , the effect of improving stability

Inactive Publication Date: 2014-11-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of HRP requires harsh reaction conditions, which is unfavorable for Lum-H 2 o 2 Universal use of luminescent systems

Method used

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  • Preparation and application of unmarked electrochemiluminescent tumor marker immunosensor
  • Preparation and application of unmarked electrochemiluminescent tumor marker immunosensor
  • Preparation and application of unmarked electrochemiluminescent tumor marker immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of AuAgNRs-Lum-Chi.

[0036] Mix 20 mL of AuNRs and 20 mL of 0.05 mol / L CTAB solution, stir at 30 °C, and sequentially add 3 mL of 0.1 mol / L AA solution, 0.5 mL of 0.05 mol / L AgNO 3 solution and 3mL 0.1 mol / L NaOH solution, stop stirring, let it stand for 1h, centrifuge, and redisperse the product in 20mL water to obtain AuAgNRs.

[0037] Mix 3 mL of AuAgNRs and 3 mL of 1×10 -3 mol / L Lum solution was mixed, stirred for 1 h, centrifuged, and the product was redispersed into 5 mL chitosan solution with a mass fraction of 0.3 % to obtain AuAgNRs-Lum-Chi solution.

[0038] The AuNRs described are 20 nm long rod-shaped AuNRs in aqueous solution.

[0039] The Chi solution is prepared by adding pure Chi to 1% acetic acid by volume.

Embodiment 2

[0040] Example 2 Preparation of AuAgNRs-Lum-Chi.

[0041] Mix 20 mL of AuNRs and 20 mL of 0.1 mol / L CTAB solution, stir at 38 °C, and sequentially add 5 mL of 0.1 mol / L AA solution, 0.8 mL of 0.05 mol / L AgNO 3 solution and 5mL 0.1 mol / L NaOH solution, stop stirring, let stand for 2h, centrifuge, and redisperse the product in 20mL water to obtain AuAgNRs aqueous solution.

[0042] Mix 4 mL of AuAgNRs and 4 mL of 1×10 -3 mol / L Lum solution was mixed, stirred for 2 h, centrifuged, and the product was redispersed into 5 mL chitosan solution with a mass fraction of 0.5 % to obtain AuAgNRs-Lum-Chi solution.

[0043] The AuNRs described are 30 nm long rod-shaped gold nanoparticles in aqueous solution.

[0044] The Chi solution is prepared by adding pure Chi to 1% acetic acid by volume.

Embodiment 3

[0045] Example 3 Preparation of AuAgNRs-Lum-Chi.

[0046] Mix 20 mL of AuNRs and 20 mL of 0.2 mol / L CTAB solution, stir at 45 °C, and sequentially add 6 mL of 0.1 mol / L AA solution, 1 mL of 0.05 mol / L AgNO 3 solution and 6mL 0.1 mol / L NaOH solution, stop stirring, let stand for 3h, centrifuge, and redisperse the product in 20mL water to obtain AuAgNRs.

[0047] Mix 5 mL of AuAgNRs and 5 mL of 1×10 -3 mol / L Lum solution was mixed, stirred for 3 h, centrifuged, and the product was redispersed into 5 mL chitosan solution with a mass fraction of 0.7 % to obtain AuAgNRs-Lum-Chi solution.

[0048] The AuNRs described are 40 nm long rod-shaped gold nanoparticles in aqueous solution.

[0049] The Chi solution is prepared by adding pure Chi to 1% acetic acid by volume.

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Abstract

The invention discloses a preparation method of an unmarked electrochemiluminescent tumor marker immunosensor and application of the immunosensor in detection of tumor markers, and belongs to the field of novel nano functional materials and biosensors. According to the preparation method of the unmarked electrochemiluminescent tumor marker immunosensor, a novel micro-nano material NH4CoPO4 is taken as a substrate so that the luminescence stability of a luminol-hydrogen peroxide luminescent system can be greatly enhanced, and meanwhile, rod-like gold@siver core-shell nanoparticles are taken as a biomimetic catalytic luminescent system, and therefore, an unmarked electrochemiluminescent immunosensor, which is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare and is used for detecting tumor makers, can be prepared.

Description

technical field [0001] The invention relates to the preparation and application of a label-free electrochemiluminescent tumor marker immunosensor. Specifically, a label-free electrochemiluminescence method based on cobalt ammonium phosphate flakes (NH 4 CoPO 4 ) and gold-silver core-shell nanorods-luminol-chitosan composite material (AuAgNRs-Lum-Chi) is an immunosensor for rapid and sensitive detection of tumor markers, which belongs to the field of new nano-functional materials and biosensor technology. Background technique [0002] Early diagnosis and early treatment of tumors are the key to improving the survival rate of cancer patients. The detection of tumor markers can reflect the biological characteristics of tumors, and is of great significance for treatment, auxiliary diagnosis, and prognosis. At present, there are many clinical detection methods for tumor markers, such as enzyme immunoassay, radioimmunoassay, etc., but these detection methods have many shortcomi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/54373G01N33/54393G01N33/57484
Inventor 张勇李建修魏琴马洪敏杜斌
Owner UNIV OF JINAN
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