Making method of unmarked electrochemical immunosensor for clenbuterol hydrochloride detection

An immunosensor and electrochemical technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problems of low yield and cumbersome process, and achieve the effects of convenient operation, simple preparation process, and improved detection sensitivity.

Active Publication Date: 2015-12-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

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  • Making method of unmarked electrochemical immunosensor for clenbuterol hydrochloride detection
  • Making method of unmarked electrochemical immunosensor for clenbuterol hydrochloride detection
  • Making method of unmarked electrochemical immunosensor for clenbuterol hydrochloride detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 MoS 2 / AgPd preparation method

[0028] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.01g of AgNO 3 and 2 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 2 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 0.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.01 g of Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 14 hours at 180°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / AgPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / AgPd solution.

Embodiment 2

[0029] Example 2 MoS 2 / AgPd preparation method

[0030] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.02g of AgNO 3and 4 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 4 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.0 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.02g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 16 hours at 150°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / AgPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / AgPd solution.

Embodiment 3

[0031] Example 3 MoS 2 / AgPd preparation method

[0032] Take 35mL of CTAB solution with a concentration of 0.1mol / L, add 0.03g of AgNO 3 and 6 mL of H at a concentration of 0.03 mol / L 2 PdCl 4 solution, after stirring for 15 minutes, continue to stir and add 6 mL of ascorbic acid solution with a concentration of 0.3 mol / L, 1.5 mL of Na with a concentration of 0.1 mol / L 2 MoO 4 solution and 0.03g Na 2 S, after stirring for 15 minutes, put it into the reaction kettle, and react for 12 hours at 220°C; after cooling to room temperature, use deionized water to wash centrifugally, and vacuum dry at 40°C to obtain MoS 2 / AgPd, which was dissolved in deionized water to obtain MoS at a concentration of 1 mg / mL 2 / AgPd solution.

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Abstract

The invention discloses a making method of an unmarked electrochemical immunosensor constructed on the basis of a molybdenum disulfide / silver palladium alloy nanocomposite material, and belongs to the technical fields of novel nanometer function materials and food safety analysis. The sensor made in the invention can be used to detect clenbuterol hydrochloride in a practical sample. The molybdenum disulfide / silver palladium alloy nanocomposite material is prepared through a one-pot technology, and then the simple, fast and sensitive unmarked electrochemical immunosensor for detecting clenbuterol hydrochloride in a meat product is made by using the excellent adsorption and electrochemical catalysis performances of the nanocomposite material.

Description

technical field [0001] The invention relates to a preparation method of a label-free electrochemical immunosensor based on a molybdenum disulfide / silver-palladium alloy nanocomposite material, and the prepared sensor can be used for the detection of clenbuterol. It belongs to the field of new nano functional materials and food safety analysis technology. Background technique [0002] Clenbuterol is a class of drugs called beta-agonists, not a specific drug. According to the "Clenbuterol" Special Rectification Program (Food Safety Office [2011] No. 14) stipulated by the Office of the Food Safety Committee of the State Council, there are 16 types of "Clenbuterol", including: ractopamine, clenbuterol, and albuterol , salbutamol sulfate, dopamine hydrochloride, cimaterol, terbutaline sulfate, phenylethanolamine A, bambuterol, zilpaterol hydrochloride, chlorprenaline hydrochloride, mabuterol, sibuterol, bromine Buterol, arformoterol tartrate, formoterol fumarate. Because they ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/5438G01N33/54393
Inventor 张勇李娇娇魏琴吴丹马洪敏
Owner UNIV OF JINAN
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