3 results about "Phenylethanolamine" patented technology

The invention discloses an enantioselective reverse micelle extraction and resolution method of a phenylethanolamine raceme drug. According to the method, an enantioselective reversed micelle formed by a carbamyl amino acid chiral surfactant in a non-aqueous solvent is used as a chiral recognition agent, the enantioselective reversed micelle and one enantiomer of a phenylethanolamine raceme drug aqueous solution are preferentially subjected to specific recognition and selectively extracted to an organic phase, and the other enantiomer is left in the aqueous phase; therefore, chiral resolution is realized. The resolution method of the phenylethanolamine racemate drug has the characteristics that the resolution process is simple, the chiral surfactant can be recycled and reused, the cost is low, the method is green and environment-friendly, and the requirement of large-scale resolution is met.

The application discloses a high-throughput screening method of phenylethanolamine-N-methyltransferase. Substrate norepinephrine and S-adenosyl methionine, PBS buffer, S-adenosyl-L-homocysteine hydrolase SAHH are added in a 96-well plate of a microplate reader, enzyme sample phenylethanolamine-N-methyltransferase is added, in a microplate shaking reactor, 20-50 DEG C, 500-1000 rpm, reaction for 1-3 h, 4 DEG C, 3700 rpm centrifugation, the supernatant is taken, compound NDCC is added again, reaction for 0-90 min, during the reaction, the microplate is taken out every 1-30 min, and the microplate reader is used to detect the absorbance value; according to the absorbance value change, the enzyme activity is defined as the change of OD405nm absorbance value per minute * 1000000 under specific conditions. The application has the characteristics of simple operation, low cost and accurate detection, and has far-reaching significance for the directed evolution of phenylethanolamine-N-methyltransferase.

The invention discloses a detection kit for detecting phenylethanolamine A based on ILAMP and a detection method thereof, and relates to the field of biological detection.According to the method, an anti-PEAA polyclonal antibody is obtained by immunizing an animal, a PEAA indirect competitive ELISA method is established on the basis, an enzyme-linked immunosorbent assay (ELISA) and fluorescent quantitative PCR are combined, an immune-PCR detection method is established, an LAMP technology is added, and the detection kit and the detection method for detecting phenylethanolamine A based on ILAMP are obtained. The phenylethanolamine A is successfully detected; compared with indirect competitive ELISA, the linear detection range, IC50 and minimum detection limit of a standard curve of the method are improved by one order of magnitude; according to the present invention, the coefficient of variation (CV) does not exceed 10%, the method has strong reliability, and the ILAMP specific detection result shows that the method has high specificity on PEAA, and has no cross reaction (CRlt; 0.04%).