76 results about "Phenylethanolamine" patented technology

A disclosed method for simultaneously analyzing and detecting residual veterinary drug compositions in animal tissue comprises: performing homogenate on an animal tissue sample by 50% acetonitrile and ethanol, performing ultrasonic extraction and hexane purifying, and performing further precipitation by acetonitrile and ethanol, concentrating, utilizing UPLC-MS/MS to perform multi-reaction monitoring (MRM) determination under the negative ion mode, and quantifying according to a standard curve and an external standard method, detecting and calculating to obtain the content of 46 residual veterinary drug compositions in animal tissue. The method is applicable to present commonly-used veterinary drugs such as 18 kinds of quinolone drugs, 22 kinds of sulfanilamide drugs, dapsone, phenylethanolamine A, amoxicillin, adamantanamine, rimantadine, ethoxyquin and the like, and is capable of performing one-step simultaneous rapid accurate detection, and the application scope of the method is enlarged.

The invention discloses a graphene-poly ionic liquid composite material, and a preparation method and application thereof. The preparation method comprises the following steps: under the protection of inert gas, conducting a free radical polymerization reaction on the graphene and an ionic liquid with ethylenic bond in the presence of an initiator; and after the reaction, washing and drying the reaction products to obtain the graphene-poly ionic liquid composite material. As the graphene-poly ionic liquid composite material has good conductivity, porous structure and high adsorption, it can be used to prepare an electrochemical sensor for detection of phenylethanolamine A. The preparation method of the invention has the advantages of simple operation, low cost and easy control of the reaction process; the obtained graphene-poly ionic liquid composite material has the advantages of high sensitivity, high stability and recyclability; and the electrochemical sensor can fast and accurately detect phenylethanolamine A.

The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E:

in which, in formula I, Z₁ is a crosslinker and Z₂ is H and, in formula II, Z₁ is H and Z₂ is a crosslinker.

The invention discloses an immune colloidal gold detection card for phenylethanolamine A and a preparation method thereof, and relates to the technical field of detection of beta-epinephrine receptor stimulants. A test paper in a shell of a detection card consists of a polyvinyl chloride (PVC) rubber plate, a sample cushion, a colloidal gold combined cushion, a coating membrane and a water sucking cushion, wherein a colloidal gold membrane is a glass cellulose membrane containing a phenylethanolamine A monoclonal antibody; the coating membrane is a nitrocellulose membrane; a line T and a line C are arranged on the coating membrane; the line T coating is provided with a phenylethanolamine A protein conjugate; and the line C is coated with a goat-anti-mouse IgG antibody. The immune colloidal gold detection card is effectively used for rapidly detecting the phenylethanolamine A, so that the detection is convenient and quick, and the detection result is accurate.

The invention discloses an enzyme-linked immunoassay kit for detecting phenylethanolamine A by a direct competition method. The enzyme-linked immunoassay kit for detecting phenylethanolamine A provided by the invention comprises an enzyme label plate coated by phenylethanolamine A specific antibodies, and a phenylethanolamine A horseradish peroxidase marker. The enzyme-linked immunoassay kit for detecting phenylethanolamine A of the invention is sensitive, rapid, accurate, and is mainly used for screening of large quantities of samples; main reagents in the kit are provided in the form of operating fluid, and the kit is convenient for using, has the characteristics of high specificity, high sensitivity, high precision, high accuracy, and the like, can rapidly detect residual phenylethanolamine A in feed and animal products.

The invention provides a chemiluminescence immunodetection kit used for detecting phenylethanolamine A, and belongs to the immunological detection field. The kit comprises an opaque white ELISA plate covered with a phenylethanolamine A/carrier protein coupling compound, a phenylethanolamine A standard substance, a phenylethanolamine A/peroxidase labeled antibody working solution, a luminous substrate liquid, a concentrate diluent and a concentrate washing liquid. The phenylethanolamine A/carrier protein coupling compound is obtained by coupling the phenylethanolamine A and a carrier protein through a mixed anhydride method or a carbodiimide method. The concentrate washing liquid comprises 0.05% of tween-20. Compared with a traditional enzyme linked immunosorbent assay method, the kit has characteristics of higher sensitivity, shorter detection time and lower cost and can be used for detection of the residue amount of the phenylethanolamine A in animal urine, blood samples, tissue, viscera, and other samples.

The present invention relates to compounds according to formula (I), a process for preparing them, the intermediate compounds of the process and the use of the compounds in the manufacture of a medicament for use in treating diseases such as ARDS, pulmonary emphysema, bronchitis, bronchiectasis, COPD, asthma and rhinitis. The compounds are beta2 adrenoreceptor agonists.

The invention discloses a phenolethanolamine substance rapid detection card and a detection method thereof, and belongs to the technical field of phenolethanolamine substance detection. The phenolethanolamine substance rapid detection card is provided with a test bar in an outer shell, and the test bar is formed by pasting a sample pad, a colloidal gold (or latex particle) membrane, a cellulose nitrate membrane and a water absorbing membrane on a supporting backboard in turn, wherein the colloidal gold (or latex particle) membrane is a glass fiber membrane containing an anti-phenylethanolamine monoclonal antibody colloidal gold (or latex particle) marker; and the cellulose nitrate membrane is provided with two detection strips in the transverse direction, one detection strip contains a phenylethanolamine protein conjugate, and the other detection strip is a quality control strip containing anti-rabbit antibody or anti-rat antibody. The phenolethanolamine substance rapid detection card has the advantage that: an immunological method is used for directly detecting the content of a phenolethanolamine feed additive in samples of feed, tissue sample, urine or serum and the like. The phenolethanolamine substance rapid detection card is easy to prepare, is convenient and quick to use, and is accurate in result.

The invention discloses an indirect-competition-law enzyme linked immunosorbent assay kit for detecting phenylethanolamine A, which comprises a phenylethanolamine A specific antibody, phenylethanolamine A and conjugates and DON-HRP-Conjugated of carrier protein. The indirect-competition-law enzyme linked immunosorbent assay kit for detecting phenylethanolamine A is sensitive, fast and accurate and is mainly used for screening examination of large quantities of samples. Main reagents in the assay kit are all provided in the form of working solution. The indirect-competition-law enzyme linked immunosorbent assay kit for detecting phenylethanolamine A has the advantages of being convenient to use, high in specificity, sensitivity, accuracy and the like and capable of fast detecting residual phenylethanolamine A in feedstuff and animal products.

R-enantiomer of Bambuterol, its preparation and therapeutic uses are disclosed. A composition includes R-Bambuterol or its therapeutically acceptable salt. A composition of R-Bambuterol includes at least 80% by weight of the R-enantiomer and not more than 20% by weight of the S-enantiomer based on a total weight of the Bmbuterol. A process includes: (a) asymmetrically reducing a suitably substituted and suitably protected bromoacetophenone compound to a chiral phenyl-bromoethanol comprising a primary bromo group and a secondary hydroxyl group; (b) displacing the bromo group by a suitably substituted and optionally protected primary amine to produce a protected chiral phenylethanolamine, and (c) removing the protecting groups to convert the protected chiral phenylethanolamine to a chiral phenylethanolamine.

The invention discloses a phenylethanolamine A molecularly imprinted membrane and a preparation method thereof. The method comprises steps of performing prepolymerization after phenylethanolamine A and methacrylic acid are mixed; adding ethylene glycol dimethacrylate and 2,2'-azodiisobutyronitrile into the system after prepolymerization to obtain a polymerization system; dipping a PVDF membrane in the polymerization system; subjecting the dipped PVDF membrane to a polymerization reaction in a nitrogen environment; and washing the PVDF membrane with methanol after the polymerization reaction is finished. Beneficial effects of the phenylethanolamine A molecularly imprinted membrane and the preparation method are that: the phenylethanolamine A molecularly imprinted membrane has advantages both of molecular imprinting technology and membrane separation technology; and the advantages comprise that (1) specific space sites and functional groups in the phenylethanolamine A molecularly imprinted membrane can achieve specific recognition of an object to be detected, and (2) compared with traditional molecularly imprinted microsphere materials, the molecularly imprinted membrane material provided by the invention is more stable, and more ordered in form, and can be used directly without tedious preparation processes such as a column packing process, etc..

The invention discloses a preparation method of a phenylethanolamine A molecularly-imprinted material, which is characterized by comprising the following steps of: (1) activating silica gel; (2) carrying out double-bond bonding on the silica gel; (3) carrying out chemical grafting on the surface of the silica gel and obtaining grafting particles; and (4) preparing the product: adding the grafting particles into 300-500mg/L phenylethanolamine A aqueous solution, adjusting the pH value of the system to be alkaline, adding a crosslinking agent and heating for reaction; after the reaction is finished, washing off template molecule phenylethanolamine A, drying and obtaining the phenylethanolamine A surface molecularly-imprinted material. Compared with the prior art, the preparation method has the advantages that the preparation process is simple, the operability is strong, and the preparation cost is low; and the molecularly-imprinted material is good in specificity, short in adsorption equilibrium time of phenylethanolamine A, short in elution time and good in elution effect.

The invention discloses a hydrophobic paper SERS substrate, a preparation method thereof and an application thereof. The natural wrinkles and a small pore diameter of filter paper allow the depositionand arrangement of metal nano particles on paper to form a large-area SERS hot spot. A composite paper-based substrate is formed by reducing and preparing a cellulose nanocrystals-silver (CNC-Ag) composite on the filter paper. Pores in the surface of the filter paper are further filled with a CNC-Ag composite material, and the reproducibility of an SERS sensor is improved. The paper-based substrate is modified with hydrophobic alkyl mercaptan, the modified substrate has a cohesive effect for a sample to be tested, and therefore, the limit of detection of an analyte is improved. That two substances including phenylethanolamine A and metronidazole can be effectively detected by the paper-based SERS substrate is proved, and the limit of detection (LOD) is as low as 5* 10(-9) mol/L and 2*10(-7) mol/L.

The invention relates to a five-conjugate testing card for detecting beta-stimulant drugs and a preparation method thereof and belongs to the technical field of beta-adrenergic receptor stimulant detection. A test strip is arranged in a casing of the testing card which is composed of a polyvinyl chloride (PVC) glue board, a sample pad, a colloidal gold combination pad, a coated film and absorbent cotton. A colloidal gold film is a glass cellulose film which contains colloidal gold markers of a clenbuterol monoclonal antibody, a ractopamine monoclonal antibody, a salbutamol monoclonal antibody, a phenylethanolamine A monoclonal antibody and a terbutaline monoclonal antibody. The coated film is a nitrocellulose film, wherein five detection lines T and a quality control line C are arranged on the coated film, and the quality control line C is coated with a rabbit anti-mouse immunoglobulin G (IgG) antibody. The five-conjugate testing card can simultaneously detect out clenbuterol, ractopamine, salbutamol, phenylethanolamine A and terbutaline, and is simple, convenient and fast in method, and accurate in result.

The invention discloses a high-temperature curing agent for phenolic resin in the fields of casting and 3D (Three Dimensional) ink-jet printing. The high-temperature curing agent for the phenolic resin is a strong-acid liquid material, the pH (Potential of Hydrogen) value of the high-temperature curing agent is smaller than 2, the content of free acid is 15 to 30 percent, and the total acidity is20 to 45 percent; the high-temperature curing agent for the phenolic resin is prepared from the following components: inorganic acid, inorganic acid salt, anhydride, urea, phenylethanolamine and othercompounds. Although common phenolic resin for casting is capable of realizing normal-temperature rapid curing and forming, both the common phenolic resin and the phenolic resin are in larger viscosity, and the specific requirements of casting and 3D ink-jet printing technologies on resin viscosity cannot be met. In order to overcome the defects of the prior art, the high-temperature curing agentfor the phenolic resin in the fields of casting and 3D ink-jet printing is successfully invented through technical innovation; casting sand formed through cross-linking and curing of the high-temperature curing agent and thermoset A-stage phenolic resin under a high-temperature condition has the advantages of high normal-temperature strength, excellent high temperature resistance, good scatteringperformance and the like.

The invention discloses a phenylethanolamine A hapten, a related artificial antigen and a monoclonal antibody; the invention simultaneously discloses preparation methods and applications of the phenylethanolamine A hapten, the related artificial antigen and the monoclonal antibody. The phenylethanolamine A hapten is the product shown in the formula 1, and the product shown in the formula 1 and a carrier protein can be connected to obtain a phenylethanolamine A antigen. The phenylethanolamine A antigen can be used for preparing a phenylethanolamine A specific antibody. The preparation methods have the advantages of simple and practicable operation, low cost and high yield of the hapten. The phenylethanolamine A artificial antigen can be used for producing the specific antibody aiming at the phenylethanolamine A by immune animals, and can be used for preparing an ELISA kit for detecting phenylethanolamine A residuals; the kit has the advantages of simple and rapid operation, large sample treatment amount, high sensitivity, good specificity, etc.

The invention discloses a method for preparing a phenylethanolamine compound intermediate. The method comprises the following steps: (1) carrying out a nitrifying reaction on 1-methyl-3-phenylpropylamine and a nitrifying reagent to obtain a 4-(4-nitrophenyl)-butan-2-amine and 4-(2-nitrophenyl)-butan-2-amine mixture; and (2) reacting the mixture obtained in step (1) with a compound of formula III,and separating the obtained reaction product to obtain the phenylethanolamine compound intermediate of formula I. The method for preparing the phenylethanolamine compound intermediate has the advantages of simplicity in operation, short route and few steps; and an o-nitro impurities generated in the nitrifying reaction can be easily removed after being reacted without separation and becoming corresponding amides, so the reaction yield and the product purity are high, and the industrial production of the product is facilitated.