Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof

A technology of phenylethanolamine and colloidal gold, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of accurate results and simple methods

Inactive Publication Date: 2013-01-30
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, the purpose of the present invention is to provide an immune colloidal gold detection card for phenylethanolamine A and a preparation method thereof in or

Method used

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  • Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof
  • Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof
  • Immune colloidal gold detection card for phenylethanolamine A and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0021] figure 1 It is the structural diagram of the immune colloidal gold detection card of phenylethanolamine A of the present invention, figure 2 It is the cross-sectional structure diagram of the test paper strip in the immune colloidal gold detection card of phenylethanolamine A of the present invention: among the figure 9 is a PVC rubber sheet; 7 is a sample pad; 8 is a colloidal gold bonding pad, and the colloidal gold bonding pad is coated Monoclonal antibody colloidal gold marker; 10 is the coated membrane, that is, nitrocellulose membrane, which is coated with phenylethanolamine A-BSA and goat anti-mouse IgG; 11 is the absorbent pad, made of absorbent material such as filter paper production.

[0022] On one end (sample end) of the PVC rubber plate 9, the sample pad 7 and the colloidal gold bonding pad 8 are adhered, and the sample pad 7 and the colloidal gold bonding pad 8 are in a side-by-side structure.

[0023] A nitrocellulose membrane 10 is adhered in the mid...

Embodiment 2

[0026] The preparation of the phenylethanolamine A colloidal gold detection card is specifically realized by the following steps:

[0027] (1) Preparation of colloidal gold solution: Take a 1 L Erlenmeyer flask, add 495 mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 (0) 5 mL, prepared into 500 mL 0.01% chloroauric acid aqueous solution, heated and boiled, then added 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) Solution 5-7 mL, continue to stir and heat, when the color of the solution turns completely transparent purple red, stop heating after maintaining for 5 min, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0028] (2) Antibody pretreatment: Centrifuge the phenylethanolamine A antibody to be labeled at 1000 r / min, 4°C for 20 min, take the supernatant, and dilute it to 1 mg / mL with 0.01 mol / L PBS; or use 0.01 mol / L PBS was diluted to 1 mg / mL, and passed through a 0.22 μm filter membrane;

[002...

Embodiment 3

[0035] Minimum detection limit test

[0036] Phenylethanolamine A minimum detection limit test

[0037] Accurately weigh 0.500 g of phenylethanolamine A standard, dissolve it in ultrapure water and dilute to 100 mL, marked as A 1 solution, take A 1 Add 1 mL of the solution to a volumetric flask and add ultrapure water to 1000 mL to form A 2 solution, take A 2 Add 1 mL of the solution to a volumetric flask and add ultrapure water to 1000 mL to form A 3 solution, the concentration of A3 solution is 5 ng / mL (5ppb). take A 3 Put 1 mL of the solution in 4 test tubes, and mark them as 1~4 respectively, add 9 mL, 4 mL, 1 mL, and 0 mL of ultrapure water to the test tubes respectively, and make the concentration of phenylethanolamine A standard solution: 0.5 ng / mL, 1 ng / mL, 2.5 ng / mL, 5 ng / mL; take another empty test tube, add 5 mL of ultrapure water, and mark it as No. 5 test tube. Take the liquid in the above test tubes 1-5 as a sample and add it dropwise to the phenylethanola...

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Abstract

The invention discloses an immune colloidal gold detection card for phenylethanolamine A and a preparation method thereof, and relates to the technical field of detection of beta-epinephrine receptor stimulants. A test paper in a shell of a detection card consists of a polyvinyl chloride (PVC) rubber plate, a sample cushion, a colloidal gold combined cushion, a coating membrane and a water sucking cushion, wherein a colloidal gold membrane is a glass cellulose membrane containing a phenylethanolamine A monoclonal antibody; the coating membrane is a nitrocellulose membrane; a line T and a line C are arranged on the coating membrane; the line T coating is provided with a phenylethanolamine A protein conjugate; and the line C is coated with a goat-anti-mouse IgG antibody. The immune colloidal gold detection card is effectively used for rapidly detecting the phenylethanolamine A, so that the detection is convenient and quick, and the detection result is accurate.

Description

technical field [0001] The invention relates to the technical field of beta-adrenoceptor agonist detection, in particular to an immune colloidal gold detection card for phenylethanolamine A and a preparation method thereof. Background technique [0002] In recent years, successive incidents of clenbuterol have become a lingering specter in China's food safety field. Last year, Hunan Province successfully cracked a case involving the production and sales of clenbuterol in Fujian, Hunan, and Zhejiang provinces, and detected phenylethanolamine A from feed that had never appeared before. Sounded the alarm bell of food safety in our country. [0003] Phenylethanolamine A, also known as "Clonpamine", can be illegally added to the feed of pigs and cattle. It can stimulate animal growth, strengthen protein deposition in animals, improve basal metabolism, and tend to decompose body fat. It can accelerate the growth rate of pigs, make their skin red and shiny, and their lean meat...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 杜道林洪霞张祯薛永来
Owner JIANGSU WISE SCI & TECH DEV
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