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Phenolethanolamine substance rapid detection card and detection method thereof

A phenylethanolamine and detection card technology, which is applied in the field of detection equipment for beta-receptor stimulants and rapid detection cards for phenylethanolamine-type illegal feed additives, can solve problems such as cumbersome operations, achieve high sensitivity, save detection costs, and be easy to use. The effect of preparation

Inactive Publication Date: 2012-01-04
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of detection equipment that can detect illegal feed additives of phenylethanolamines, directly apply the content of the illegal feed additives of phenylethanolamines in samples such as the method detection feed of immunology or tissue (muscle, liver and kidney), overcome Due to the shortcomings of the existing chromatographic detection methods such as cumbersome operation, a detection method based on immunological specificity should be established

Method used

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  • Phenolethanolamine substance rapid detection card and detection method thereof
  • Phenolethanolamine substance rapid detection card and detection method thereof
  • Phenolethanolamine substance rapid detection card and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A quick test card for phenylethanolamine substances, which is provided with a test strip 2 in the test card shell 1, the structure of the test card shell 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat thin shell shell, 70mm long, 20mm wide, 5mm thick, and the thin shell wall thickness is 1mm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0024] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 60mm, the width of the strip is about 4mm, and the thickness is about 2.5mm. The test strip 2 has a multi-layer structure: the bottom layer is a supporting backboard 5, which is a polyvinyl chloride plast...

Embodiment 2

[0027] Samples were processed as follows:

[0028] A. Urine or serum: Urine or serum is directly used as a test sample. If the urine sample is turbid, it must be filtered through filter paper or centrifuged for 10 minutes (15°C, 4000r / min).

[0029] B. Feed: Weigh 0.5 g of feed sample, add 1 mL of sample extract, stir in the test tube for 1 min, and after standing for 10 min, take the clear supernatant as the test sample.

[0030] C. Tissue samples (muscle, liver or kidney): Take 1g of shredded or homogenized animal tissue, meat, liver or kidney samples, put them in a test tube containing 2mL of sample extract, tighten the cap tightly, and shake After the sample was left for 1 min, it was cooled to room temperature in a 100°C water bath for 5 minutes, and the clear middle part was taken as a test sample.

[0031] The sample extract PBST is a 0.01mol / L PBST solution containing 0.15mol / L NaCl and 0.5% Tween-20 pH7.4.

[0032] When using the rapid detection card for phenylethan...

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Abstract

The invention discloses a phenolethanolamine substance rapid detection card and a detection method thereof, and belongs to the technical field of phenolethanolamine substance detection. The phenolethanolamine substance rapid detection card is provided with a test bar in an outer shell, and the test bar is formed by pasting a sample pad, a colloidal gold (or latex particle) membrane, a cellulose nitrate membrane and a water absorbing membrane on a supporting backboard in turn, wherein the colloidal gold (or latex particle) membrane is a glass fiber membrane containing an anti-phenylethanolamine monoclonal antibody colloidal gold (or latex particle) marker; and the cellulose nitrate membrane is provided with two detection strips in the transverse direction, one detection strip contains a phenylethanolamine protein conjugate, and the other detection strip is a quality control strip containing anti-rabbit antibody or anti-rat antibody. The phenolethanolamine substance rapid detection card has the advantage that: an immunological method is used for directly detecting the content of a phenolethanolamine feed additive in samples of feed, tissue sample, urine or serum and the like. The phenolethanolamine substance rapid detection card is easy to prepare, is convenient and quick to use, and is accurate in result.

Description

technical field [0001] The invention relates to a device for detecting β-receptor stimulants, in particular to a rapid detection card for phenylethanolamine illegal feed additives and a detection method thereof, belonging to the technical field of detection of phenylethanolamine substances. Background technique [0002] β-receptor agonists are a class of chemically synthesized phenylethanolamines, which can be divided into three types: aniline type (such as clenbuterol), phenolic type (such as albuterol) and quinone type (such as terbutaline). category. This type of drug was mainly used to prevent and treat bronchial asthma and convulsions in humans and animals in the early stage of development. Later, it was found that this type of drug can redistribute nutrients after being added to feed, and can significantly increase the lean meat rate of animals. It was once used as a drug to promote Growth additives have received widespread attention, but after eating animal-derived f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 周坚吴文晔熊晓芳
Owner 无锡安迪生物工程有限公司
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